Citation: LI Lingyun, XU Xiaomin, LIN Huan, ZHAO Wen, YE Rong, HUANG Xiaodong, ZHENG Shuning, LIU Xinyan, LIU Su, XU Donghui. Rapid detection of 248 pesticide residues in vegetables by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(9): 835-849. doi: 10.3724/SP.J.1123.2016.04004 shu

Rapid detection of 248 pesticide residues in vegetables by ultra high performance liquid chromatography-tandem mass spectrometry

LI Lingyun ^1, ,
XU Xiaomin ¹ ,
LIN Huan ¹ ,
ZHAO Wen ² ,
YE Rong ³ ,
HUANG Xiaodong ¹ ,
ZHENG Shuning ¹ ,
LIU Xinyan ¹ ,
LIU Su ¹ ,
XU Donghui ¹

1.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Vegetable Products(Beijing), Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, China;
2.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
3.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

Received Date: 5 April 2016

Fund Project: National Vegetable Product Quality and Safety Risk Assessment Project (No. GJFP2016002).

A multiresidue analytical method for rapid detection of 248 pesticide residues in vegetables using an ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique was successfully developed. The vegetable samples were extracted by acetonitrile. After salting out, the solvent extracts were analyzed by UHPLC-MS/MS directly. And further purification was not needed. The positive and negative ion modes and multiple reaction monitoring (MRM) mode were used to identify and quantify the 248 pesticide residues in vegetables. The results showed that 245 pesticides had good linearity within their respective linear ranges (r>0.99). Except four pesticides (carbosulfan, cyromazine, tribenuron-methyl, quinclorac), the average recoveries of the other 244 pesticides at three spiked levels ranged from 63.0% to 126.4% with the relative standard deviations (RSDs) from 0.5% to 26.7%. The limits of quantification of this method were in the range of 0.001-0.030 mg/kg. In comparison with other pretreatment methods for pesticide residues, this method not only shortened the time of sample pretreatment, but also enhanced significantly the recoveries of pesticides. The results in this study suggested that it is a simple, rapid, sensitive and accurate method for pesticide residue determination, and suitable for rapid screening of pesticide residues in vegetables.
- ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS),
- pesticide residues,
- vegetables
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