Citation: LI Yao, CHEN Jingya, WU Yulan, HAN Shuying, YU Hongli, WU Hao, CHI Yumei. Determination of the kinetics of photo-isomerization and equilibrium constant of resveratrol by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2016, 34(8): 783-787. doi: 10.3724/SP.J.1123.2016.03008 shu

Determination of the kinetics of photo-isomerization and equilibrium constant of resveratrol by high performance liquid chromatography

1.
Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China

Corresponding author: WU Hao, CHI Yumei,
Received Date: 6 March 2016

Fund Project:

Trans-resveratrol (C₁₄H₁₂O₃, 5-(4-hydroxystyryl)benzene-1, 3-diol), with a variety of physiological activities and abundant sources, shows instability to light irradiation. The control solution was balanced by photo-isomerization and high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of trans-resveratrol and cis-resveratrol. The methodology study showed good linear correlations (R²=0.999) in the range of 1.5-60 μ mol/L of trans-resveratrol and 1.5-55 μ mol/L of cis-resveratrol which conforms to the request of content determination. The method was used to study the kinetics and equilibrium constant of photo-isomerization. The results showed that trans-resveratrol protected from light was stable in neutral, weak acid and ethanol media at room temperature. The photo-isomerization equilibrium constant was 11.7±0.3 at 25 ℃ in dilute ethanol solution. The method of making the standard curve by photo-isomerization equilibrium solution is simple, convenient and practicable. The stability of trans-resveratrol is related to the medium. When pH value is less than 7, the photo-isomerization is a first order reaction.
- high performance liquid chromatography (HPLC),
- photo-isomerization,
- resveratrol,
- kinetics,
- equilibrium constant
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