Citation: DING Bo, WANG Zhiyuan, XIE Jianjun, ZENG Guangfeng, LI Xun, SHE Zhigang, CHEN Wenrui. Determination of 24 antihyperglycemic, antihypertensive and antihyperlipidemic drugs illegally added in health food samples by liquid chromatography-quadrupole- time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(6): 583-590. doi: 10.3724/SP.J.1123.2016.02016 shu

Determination of 24 antihyperglycemic, antihypertensive and antihyperlipidemic drugs illegally added in health food samples by liquid chromatography-quadrupole- time of flight mass spectrometry

DING Bo ^1,2 ,
WANG Zhiyuan ¹ ,
XIE Jianjun ¹ ,
ZENG Guangfeng ¹ ,
LI Xun ¹ ,
SHE Zhigang ² ,
CHEN Wenrui ^1,*,,

1.
Guangdong Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
2.
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China

Corresponding author: CHEN Wenrui, chenciq119@163.com
Received Date: 21 February 2016

Fund Project: Guangzhou Project of Science & Technology Planning No.201300000062

A liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) coupled with QuEChERS pretreatment analytical method was established for the identification and quantification of 24 antihyperglycemic, antihypertensive and antihyperlipidemic drugs illegally added in health foods. The extraction conditions, including the volume ratio of ACN and H₂O, the amount of MgSO₄ & NaCl used, and the amount of C18 used, were optimized by response surface methodology. The quantitative analysis was based on the accurate masses of the first level of parent molecular ions and isotopic patterns, and accurate masses of the second level of fragment ions and a priori known retention times. The established QuEChERS pretreatment method combined with LC-Q-TOF-MS was effective for the simultaneous characterization and quantification of antihyperglycemic, antihypertensive and antihyperlipidemic drugs with high selectivity, a high linearity (r²>0.997) in wide range, low LODs and LOQs, and high precision. The method was successfully applied to determinate the drugs illegally added in the fifteen health food samples, the five of which were positive samples.
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