Citation: SONG Ge, KANG Meijuan, YANG Jinbao. Determination of 1,3-dioleoyl-2-palmitoyl triglyceride in infant formula milk powder by normal-phase liquid chromatography[J]. Chinese Journal of Chromatography, ;2016, 34(3): 351-355. doi: 10.3724/SP.J.1123.2015.11013 shu

Determination of 1,3-dioleoyl-2-palmitoyl triglyceride in infant formula milk powder by normal-phase liquid chromatography

1.
National Dairy Testing Center, Northeast Agriculture University, Harbin 150028, China

Received Date: 9 November 2015

Methods for the determination of 1,3-dioleoyl-2-palmitoyl triglyceride (OPO) in infant formula milk powder by normal-phase liquid chromatography (NP-LC) and reversed-phase liquid chromatography (RP-LC) were established and compared. The analytes in the infant formula were extracted by ethyl ether and petroleum ether, enriched by a rotary evaporator, and separated on silver-ion column and C18 column and detected with an evaporative light scattering detector (ELSD). OPO and 1,2-dioleoyl-3-palmitoyl triglyceride (OOP) can be basically separated on a silver-ion column with a mixed solution of dichloromethane-acetone as mobile phase by gradient elution. The recoveries were 93.0%-101.5%, the relative standard deviations (RSD) were 2.89%-4.56%, and the limit of detection was 20 mg/kg. This method is accurate and stable for the determination of OPO in the infant formula milk powder.
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