Citation: ZHU Feng, JI Wenliang, RUAN Liping, LIU Hualiang, LI Fang. Simultaneous determination of 13 β-lactam residues in water by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(3): 299-305. doi: 10.3724/SP.J.1123.2015.10028 shu

Simultaneous determination of 13 β-lactam residues in water by high performance liquid chromatography-tandem mass spectrometry

1.
Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China

Received Date: 18 October 2015

Fund Project: 江苏科技厅科技支撑计划项目(BE2010745) (BE2010745)五"科教兴卫工程项目(ZX201109). (ZX201109)

A rapid method for the simultaneous screening and determination of 13 β-lactam residues in water was developed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After high speed centrifugation, the sample was filtered through a 0.22 μm microporous filter membrane, and then separated on an Atlantis dC-C₁₈ column (150 mm×4.6 mm, 5 μm, Waters) with the gradient elution of water (containing 0.05% (v/v) formic acid) and methanol-acetonitrile (4 : 6, v/v). The electrospray ionization (ESI) source in positive ion mode was used for multiple reaction monitoring (MRM) mode. The 13 β-lactam chemical components showed good linear relationships in their concentration ranges with the correlation coefficients (r²) more than 0.99. The recoveries were all in the range of 81.4%-102.3%, and the relative standard deviations (RSDs) were all less than 11%(n=7). The limits of detection (LODs) were in the range of 1.5-10 ng/L. This method is rapid, simple, sensitive, accurate, and provides the basis for the investigation and monitoring of the pollution levels of the antibiotics in water.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- β-lactam residues,
- antibiotics,
- water
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