Citation: SHI Ao. Determination of 23 antibiotics and 3 β-agonists in livestock drinking water by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid-phase extraction[J]. Chinese Journal of Chromatography, ;2016, 34(2): 176-183. doi: 10.3724/SP.J.1123.2015.09021 shu

Determination of 23 antibiotics and 3 β-agonists in livestock drinking water by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid-phase extraction

SHI Ao ^,

1.
Beijing Monitoring Station for Animal Husbandry Environment, Beijing 102200, China

Corresponding author: SHI Ao,
Received Date: 18 September 2015

Fund Project: 北京市农委基金项目(PXM2014 036240 000012). (PXM2014 036240 000012)

A method has been developed for the simultaneous determination of 23 antibiotics (four categories) and 3 β-agonists in livestock drinking water using solid-phase extraction and ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI MS/MS). The samples were adjusted pH to 5.0, added Na₂EDTA, enriched and cleaned-up by an HLB solid-phase extraction cartridge. The target compounds were confirmed and quantified by UPLC-ESI MS/MS with external standard method for the antibiotics and internal standard method for the β-agonists. The recoveries were assessed by using lab tap water as matrix. The average recoveries of the 23 antibiotics and the 3 β-agonists were in the range of 50.7%-104.6% and the relative standard deviations (RSDs) were 2.6%-8.8%(n=3). Under the optimal conditions, the calibration curves of the 23 antibiotics and the 3 β-agonists showed good linearity with the correlation coefficients better than 0.994. The limits of detection (LODs, S/N≥3) ranged from 0.01-0.20 ng/L. The developed method was applied to analyze the livestock drinking waters in 36 Beijing intensive livestock farms. The results showed that some antibiotics were detected.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- solid-phase extraction,
- antibiotics,
- β-agonists,
- livestock drinking water
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