Citation: HAO Feiran, FU Bin, ZHANG Yangjun, QIAN Xiaohong. Recent advances in preparative electromigration techniques for proteins[J]. Chinese Journal of Chromatography, ;2015, 33(12): 1226-1233. doi: 10.3724/SP.J.1123.2015.09010 shu

Recent advances in preparative electromigration techniques for proteins

1.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China

Corresponding author: ZHANG Yangjun, QIAN Xiaohong,
Received Date: 14 September 2015

Fund Project: 国家重大科学计划项目(2012CB910603) (2012CB910603)国家高技术研究发展规划项目(2012AA020202) (2012AA020202)国家重大科学仪器设备开发专项项目(2012YQ12004407,2011YQ030139,2011YQ06008408,2013YQ14040506) (2012YQ12004407,2011YQ030139,2011YQ06008408,2013YQ14040506)国家自然科学基金项目(21275519,20735005,31100591). (21275519,20735005,31100591)

Preparative electromigration techniques are a category of separation and preparation techniques based on differential electromigration principle. These techniques are widely applied in biomacromolecule and proteome researches. Related techniques mainly include preparative electrophoresis, preparative electrochromatography, preparative isoelectric focusing and free flow electrophoresis and so on. We review each kind of the preparative electromigration techniques on its apparatus designs, applications, advantages and disadvantages in detail. Microscale preparative electrophoresis is a hot spot in recent years. Its high resolution, high recovery and high efficiency make it a more prominent role in the biological sample analysis. In this review, we focus on some advances in microscale preparative electrophoresis and also give an outlook on preparative electromigration techniques in the future.
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