Citation: SUN Jian, LI Limin, HU Qing, MAO Xiuhong, JI Shen. Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(2): 184-188. doi: 10.3724/SP.J.1123.2015.08027 shu

Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry

1.
Shanghai Institute for Food and Drug Control, Shanghai 201203, China

Corresponding author: JI Shen,
Received Date: 24 August 2015

Fund Project:

An analytical method was developed for the determination of ginkgolic acids in ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry. A chromatographic column, Agilent Poroshell 120 EC-C18(50 mm×3.0 mm, 2.7 μ m), was used with methanol-1% acetic acid (90 : 10, v/v) as the mobile phase. The ginkgo acids were detected by electrospray ionization mass spectrometry in negative mode with multiple reaction monitoring (MRM) mode. Ginkgo acids C13 : 0, C15 : 1 and C17 : 1 possessed good linear correlations in the mass concentration range from 2 to 200 μ g/L, with the correlation coefficients more than 0.999. The mean recoveries at the spiked levels of 5, 20 and 100 μ g/L were in the range of 86.3%-114.3%, and the RSDs were 0.5%-13.6%. The limits of detection and quantification were 0.003-0.08 μ g/g and 0.01-0.19 μ g/g, respectively. The method was successfully applied to the analysis of actual samples.
1. [1]
  [1] Qi H Z, Zhou X J, Wang M X. Hebei Journal of Traditional Chinese Medicine, 2013, 35(12): 1899 齐惠珍, 周霞瑾, 王明霞. 河北中医, 2013, 35(12): 1899
2. [2]
  [2] Liu P P, Pan S H. China Journal of Chinese Materia Medica, 2012, 37(3): 274 刘平平, 潘苏华. 中国中药杂志, 2012, 37(3): 274
3. [3]
  [3] Wu X Y, Yang L Q, Chen J. Acta Pharmaceutica Sinica, 2003, 38(11): 846 吴向阳, 仰榴青, 陈钧. 药学学报, 2003, 38(11): 846
4. [4]
  [4] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: China Medical Science Press, 2010: 392 国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社, 2010: 392
5. [5]
  [5] European Pharmacopoeia 8.0: 1257
6. [6]
  [6] U. S. Pharmacopoeia 38: 6064
7. [7]
  [7] Nie L X, Lu J. Chinese Journal of Pharmaceutical Analysis, 2005, 25(8): 909 聂黎行, 鲁静. 药物分析杂志, 2005, 25(8): 909
8. [8]
  [8] Yao J B, Fang L, Wang R W, et al. Chinese Journal of Pharmaceutical Analysis, 2012, 32(11): 2055 姚建标, 方玲, 王如伟, 等. 药物分析杂志, 2012, 32(11): 2055
9. [9]
  [9] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: China Medical Science Press, 2015: 416 国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社, 2015: 416
10. [10]
  [10] Li L C, Yin Z F, Yang Y Q, et al. China Practical Medicine, 2015, 10(1): 233 李兰崇, 尹志芳, 杨宇强, 等. 中国实用医药, 2015, 10(1): 233
11. [11]
  [11] Zhang Z Y, Wang H N, Wang K Y, et al. Chinese Traditional Patent Medicine, 2012, 34(10): 2045 张志勇, 王海宁, 王克英, 等. 中成药, 2012, 34(10): 2045
12. [12]
  [12] Yao X, Zhou G S, Tang Y P, et al. Biomed Res Int, 2013, 2013: 582591
13. [13]
  [13] Wang L L, Jia Y L, Pan Z F, et al. Chinese Journal of Chromatography, 2008, 26(6): 731 王丽丽, 贾一律, 潘再法, 等. 色谱, 2008, 26(6): 731
14. [14]
  [14] Yang X M, He J, Liu Y J, et al. Lishizhen Medicine and Materia Medica Research, 2009, 20(10): 2453 杨小明, 何军, 柳艳君, 等. 时珍国医国药, 2009, 20(10): 2453
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沈阳化工大学材料科学与工程学院沈阳 110142