Citation: GUO Shuang, ZHU Chenqi, YANG Yaya, QIU Bailing, WU Di, LIANG Qihui, HE Jiayuan, HAN Nanyin. Effects of carrier liquid and flow rate on the separation in gravitational field-flow fractionation[J]. Chinese Journal of Chromatography, ;2016, 34(2): 146-151. doi: 10.3724/SP.J.1123.2015.08021 shu

Effects of carrier liquid and flow rate on the separation in gravitational field-flow fractionation

GUO Shuang ¹ ,
ZHU Chenqi ¹ ,
YANG Yaya ¹ ,
QIU Bailing ¹ ,
WU Di ¹ ,
LIANG Qihui ¹ ,
HE Jiayuan ² ,
HAN Nanyin ^1,,

1.
1. School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;
2.
2. China Agricultural University, Beijing 100193, China

Corresponding author: HAN Nanyin,
Received Date: 19 August 2015

Gravitational field-flow fractionation is the simplest field-flow fractionation technique in terms of principle and operation. The earth's gravity is its external field. Different sized particles are injected into a thin channel and carried by carrier fluid. The different velocities of the carrier liquid in different places results in a size-based separation. A gravitational field-flow fractionation (GrFFF) instrument was designed and constructed. Two kinds of polystyrene (PS) particles with different sizes (20 μ m and 6 μ m) were chosen as model particles. In this work, the separation of the sample was achieved by changing the concentration of NaN₃, the percentage of mixed surfactant in the carrier liquid and the flow rate of carrier liquid. Six levels were set for each factor. The effects of these three factors on the retention ratio (R) and plate height (H) of the PS particles were investigated. It was found that R increased and H decreased with increasing particle size. On the other hand, the R and H increased with increasing flow rate. The R and H also increased with increasing NaN₃ concentration. The reason was that the electrostatic repulsive force between the particles and the glass channel wall increased. The force allowed the samples approach closer to the channel wall. The results showed that the resolution and retention time can be improved by adjusting the experimental conditions. These results can provide important values to the further applications of GrFFF technique.
- field-flow fractionation (FFF),
- gravitational field-flow fractionation (GrFFF),
- retention ratio,
- plate height,
- carrier liquid,
- flow rate,
- polystyrene (PS) particles
1. [1]
  [1] Mazanec K, Dycka F, Bobalova J. J Sci Food Argic, 2011, 91(15): 2756
2. [2]
  [2] Lee S, Kang D Y, Park M, et al. Anal Chem, 2011, 83(9): 3343
3. [3]
  [3] Bolea E, Gorriz M P, Bouby M, et al. J Chromatogr A, 2006, 1129: 236
4. [4]
  [4] Baalousha M, Stolpe B, Lead J R. J Chromatogr A, 2011, 1218: 4078
5. [5]
  [5] Yohannes G, Jussila M, Hartonen K, et al. J Chromatogr A, 2011, 1218: 4104
6. [6]
  [6] Quan C, Liu P P, Jin J S. Chemistry Online, 2013, 76(1): 52 全灿, 刘攀攀, 金君素. 化学通报, 2013, 76(1): 52
7. [7]
  [7] Rasouli S. J Mater Sci Eng A, 2011, 1: 37
8. [8]
  [8] Park M R, Kang D Y, Chmelik J, et al. J Chromatogr A, 2008, 1209: 206
9. [9]
  [9] Woo I S, Jung E C, Lee S. Talanta, 2015, 132: 945
10. [10]
  [10] Plocková J, Chmelík J. J Chromatogr A, 2006, 1118: 253
11. [11]
  [11] Sanz R, Puignou L, Reschiglian P, et al. J Chromatogr A, 2001, 919: 339
12. [12]
  [12] Shao L Y, Jiang D L. Journal of Jilin Normal University: Natural Science Edition, 2011, 32(1): 91 邵丽颖, 蒋东霖. 吉林师范大学学报: 自然科学版, 2011, 32(1): 91
13. [13]
  [13] Jiang D L, Shao L Y. Journal of Jilin Normal University: Natural Science Edition, 2009, 30(1): 78 蒋东霖, 邵丽颖. 吉林师范大学学报: 自然科学版, 2009, 30(1): 78
14. [14]
  [14] Plockva J, Matulik F, Chmelik J. J Chromatogr A, 2002, 995: 95
15. [15]
  [15] Plockva J, Chmelik J. J Chromatogr A, 2001, 918: 361
16. [16]
  [16] Sant H J, Gale B K. J Chromatogr A, 2006, 1104: 282
1. [1]
  Zeyi Yan , Ruitao Liu , Xinyu Qi , Yuxiang Zhang , Lulu Sun , Xiangyuan Li , Anchao Feng . Exploration of Suspension Polymerization: Preparation and Fluorescence Stability of Perovskite Polystyrene Microbeads. University Chemistry, 2025, 40(4): 72-79. doi: 10.12461/PKU.DXHX202405110
2. [2]
  Chengqian Mao , Yanghan Chen , Haotong Bai , Junru Huang , Junpeng Zhuang . Photodimerization of Styrylpyridinium Salt and Its Application in Silk Screen Printing. University Chemistry, 2024, 39(5): 354-362. doi: 10.3866/PKU.DXHX202312014
3. [3]
  Ruoqian Zhang , Chaoqun Mu , Yali Hou , Mingming Zhang . 四苯乙烯基多组分金属有机笼的构筑及其固态发光性能研究. University Chemistry, 2025, 40(8): 277-283. doi: 10.12461/PKU.DXHX202410027
4. [4]
  Wenjun Yang , Qiaoling Tan , Wenjiao Xie , Xiaoyu Pan , Youyong Yuan . Construction and Characterization of Calcium Alginate Microparticle Drug Delivery System: A Novel Design and Teaching Practice in Polymer Experiments. University Chemistry, 2025, 40(3): 371-380. doi: 10.12461/PKU.DXHX202405150
5. [5]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
6. [6]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
7. [7]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
8. [8]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
9. [9]
  Yongmei Chen , Lidan Zhang , Shunlai Li , Chunting Zhang , Meng Cui , Qinghong Xu , Lan Jin , Chunchuang Li , Zhi Lv . Development of a National First-Class Course of “University Chemistry Experiment” Based on MOOCs. University Chemistry, 2024, 39(7): 8-12. doi: 10.3866/PKU.DXHX202404017
10. [10]
  Fan Yu , Aihua Li , Yun Liu , Tianrong Zhu , Liang Wang , Junhui Xu , Yazhen Wang . Exploration and Practice in Developing a Premier Course in Inorganic and Analytical Chemistry. University Chemistry, 2024, 39(8): 36-43. doi: 10.3866/PKU.DXHX202312037
11. [11]
  Shuyong Zhang , Wenfeng Jiang , Changsheng Lu , Genrong Qiang , Yongmei Liu , Xiangyang Tang , Dongcheng Liu , Lili Zhang . Suggestions on Construction and Evaluation Standards for First-Class Chemical Experiment Teaching. University Chemistry, 2025, 40(5): 9-14. doi: 10.12461/PKU.DXHX202502114
12. [12]
  Lan Ma , Cailu He , Ziqi Liu , Yaohan Yang , Qingxia Ming , Xue Luo , Tianfeng He , Liyun Zhang . Magical Surface Chemistry: Fabrication and Application of Oil-Water Separation Membranes. University Chemistry, 2024, 39(5): 218-227. doi: 10.3866/PKU.DXHX202311046
13. [13]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
14. [14]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
15. [15]
  Peipei Sun , Jinyuan Zhang , Yanhua Song , Zhao Mo , Zhigang Chen , Hui Xu . Built-in Electric Fields Enhancing Photocarrier Separation and H₂ Evolution. Acta Physico-Chimica Sinica, 2024, 40(11): 2311001-0. doi: 10.3866/PKU.WHXB202311001
16. [16]
  Hongpeng He , Mengmeng Zhang , Mengjiao Hao , Wei Du , Haibing Xia . Synthesis of Different Aspect-Ratios of Fixed Width Gold Nanorods. Acta Physico-Chimica Sinica, 2024, 40(5): 2304043-0. doi: 10.3866/PKU.WHXB202304043
17. [17]
  Nana Wang , Gaosheng Zhang , Huosheng Li , Tangfu Xiao . Discussion on the Teaching Reform of Environmental Functional Materials within the Context of “Double First-Class” Initiative: Emphasizing the Integration of Industry, Academia, Research, and Application. University Chemistry, 2024, 39(6): 137-144. doi: 10.3866/PKU.DXHX202312010
18. [18]
  Yue Zhang , Chengxin Shang , Caihong Zhang , Jikai Pei , Lihong Shi , Pengfei Gao , Zhiqi Jia , Songbai Wang , Junsheng Hao , Guomei Zhang , Wei Guo . Reforms and Practices of the Chemistry Experimental Teaching Demonstration Center under the Background of National First-Class Major Construction. University Chemistry, 2024, 39(7): 207-210. doi: 10.12461/PKU.DXHX202405134
19. [19]
  Peifeng Su , Xin Lu . Development of Undergraduate Quantum Mechanics Module in Chemistry Department under the “Double First Class” Initiative. University Chemistry, 2024, 39(8): 99-103. doi: 10.3866/PKU.DXHX202401087
20. [20]
  Yuan Zhuang , Wenhui Li , Jie Li . Curriculum Reform of “Chemical Composition Analysis of Materials” under Background of First-Class Discipline Construction. University Chemistry, 2025, 40(5): 283-290. doi: 10.12461/PKU.DXHX202407070

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(371)
HTML views(37)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142