Citation: ZONG Yanping, LI Jinghua, SUN Wei, LIU Guixia, LU Jinghua, SHAN Guangzhi. Determination of succinic acid in desvenlafaxine succinate by high performance ion-exclusion chromatography and high performance ion-exchange chromatography[J]. Chinese Journal of Chromatography, ;2016, 34(2): 189-193. doi: 10.3724/SP.J.1123.2015.08017 shu

Determination of succinic acid in desvenlafaxine succinate by high performance ion-exclusion chromatography and high performance ion-exchange chromatography

ZONG Yanping ^1,2 ,
LI Jinghua ² ,
SUN Wei ² ,
LIU Guixia ² ,
LU Jinghua ^1,, ,
SHAN Guangzhi ^2,,

1.
1. College of Medicine, Liaoning Medical University, Jinzhou 121000, China;
2.
2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China

Corresponding author: LU Jinghua, SHAN Guangzhi,
Received Date: 17 August 2015

Fund Project: 科技部新药创制重大专项平台项目(2012ZX09301002-001-019) (2012ZX09301002-001-019)辽宁医学院横向课题(LYHX2013001). (LYHX2013001)

New methods were developed for the determination of succinic acid in desvenlafaxine succinate (DVS) by high performance ion-exclusion chromatography (HPIEC) and high performance ion-exchange chromatography (HPIC). HPIEC and HPIC methods were used separately to determinate the succinic acid in DVS. With HPIEC, the sample was diluted with 2.50×10^-3mol/L sulfuric acid solution and filtrated by 0.22 μ m polyether sulfone filter membrane, and then analyzed by HPIEC directly without any further pretreatment. The analytical column was Phenomenex Rezex ROA-organic Acid H+(8%)(300 mm×7.8 mm). The mobile phase was 2.50×10^-3mol/L sulfuric acid solution at the flow rate of 0.5 mL/min. The column temperature was set at 40 ℃, and the detection wavelength was 210 nm. The injection volume was 10 μ L. The assay was quantified by external standard method. With HPIC, the sample was diluted with ultrapure water and filtrated by 0.22 μ m polyether sulfone filter membrane, and then analyzed by HPIC directly without any further pretreatment. The analytical column was Dionex IonPac AS11-HC (250 mm×4 mm) with a guard column IonPacAG11-HC (50 mm×4 mm). Isocratic KOH elute generator was used at the flow rate of 1.0 mL/min. The detection was performed by a Dionex suppressed (DIONEX AERS 500 4-mm) conductivity detector. The injection volume was 10 μ L. The content computation was performed with peak area external reference method. The results of HPIEC method for succinic acid were 28.8%, 28.9% and 28.9%, while the results of HPIEC method were 28.2%, 28.6% and 28.6%. The results of HPIEC and HPIC methods were not significantly different. The two methods can both be used to determine the contents of succinic acid in DVS. The surveillance analytical method should be chosen according to the situation.
1. [1]
  [1] An F R, Cui L. Pharmaceutical Care and Research, 2010, 10 (1): 47 安富荣, 崔岚. 药学服务与研究, 2010, 10(1): 47
2. [2]
  [2] Nichols A I, Fatato P, Shenouda M, et al. J Clin Pharmacol, 2009, 49(2): 219
3. [3]
  [3] Meng J P. China Pharmacy, 2012, 23(10): 927 孟江平. 中国药房, 2012, 23(10): 927
4. [4]
  [4] Chen L X, Chen Q Y, Jiang F. Analytical Instrumentation, 2010, (5): 43 陈立新, 陈麒宇, 江帆. 分析仪器, 2010, (5): 43
5. [5]
  [5] Wen S L, Liu J H, Guo H F, et al. Journal of Instrument Analysis, 2009, 28(6): 692 韦寿莲, 刘君红, 郭海福, 等. 分析测试学报, 2009, 28(6): 692
6. [6]
  [6] Liu J, Li J, Nie L X, et al. Journal of Pharmaceutical Analysis, 2012, 32(10): 1774 刘静, 李静, 聂黎行, 等. 药物分析杂志, 2012, 32(10): 1774
7. [7]
  [7] Liu R, Nie L X, Sun H, et al. Chinese Journal Analytical Chemistry, 2012, 40(3): 489 刘瑞, 聂黎行, 孙焕, 等. 分析化学, 2012, 40(3): 489
8. [8]
  [8] Yao X P, Li X P, Chen X H, et al. Chinese Journal of Health Laboratory Technology, 2008, 18(5): 801 姚浔平, 李小平, 陈晓红, 等. 中国卫生检验杂志, 2008, 18(5): 801
9. [9]
  [9] Lin X J, Wei W, He Z G, et al. Chinese Journal of Chromatography, 2014, 32(3): 304 林晓婕, 魏巍, 何志刚, 等. 色谱, 2014, 32(3): 304
10. [10]
  [10] Li M, Yang Y, Wang Z Z, et al. Progress in Modern Biomedicine, 2011, 11(23): 4722 李敏, 杨滢, 王喆之. 现代医学进展, 2011, 11(23): 4722
11. [11]
  [11] Ding R, Zhou C M, Ji H, et al. Journal of Pharmaceutical Analysis, 2012, 32(2): 350 丁锐, 周长明, 纪宏, 等. 药物分析杂志, 2012, 32(2): 350
12. [12]
  [12] Zhu H J, Zhang Z N, Yang Y W, et al. Chinese Journal of Health Laboratory Technology, 2013, 23(9): 2059 朱会卷, 张卓娜, 杨艳伟, 等. 中国卫生检验杂志, 2013, 23(9): 2059
13. [13]
  [13] Huang B S, Cao Y, Li J, et al. China Journal of Hospital Pharmacy, 2005, 25(11): 1037 黄必胜, 曹艳, 李娟, 等. 中国医院药学杂志, 2005, 25(11): 1037
14. [14]
  [14] Zhong Z J, Ding J H, Qiu C F, et al. Journal of Instrumental Analysis, 2010, 29(6): 589 衷张菁, 丁健桦, 邱昌福, 等. 分析测试学报, 2010, 29(6): 589
15. [15]
  [15] Mou S F, Liu K N, Ding X J. Ion Chromatographic Method and Its Application. 2nd ed. Beijing: Chemical Industry Press, 2005: 135 牟世芬, 刘克纳, 丁晓静. 离子色谱方法及应用. 2版. 北京: 化学工业出版社, 2005: 135
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