Citation: LI Jing, GUO Zhanchen, ZHAO Xu, MA Yuhua, HAN Ping, FENG Shun. Simultaneous determination of four drugs for kidney diseases in urine by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(11): 1210-1213. doi: 10.3724/SP.J.1123.2015.07022 shu

Simultaneous determination of four drugs for kidney diseases in urine by high performance liquid chromatography

1.
Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education and Xinjiang Uyghur Autonomous Region, Xinjiang University, Urumqi 830046, China

Corresponding author: FENG Shun,
Received Date: 21 July 2015

Fund Project: 新疆大学2014年国家大学生创新创业实训项目(201410755015). (201410755015)

A high performance liquid chromatographic (HPLC) method was proposed for the simultaneous determination of four drugs for kidney disease, enalapril, triamterene, furosemide and valsartan. After proteins being removed by acetone precipitation method, freeze drying and redissolving in mobile phase, the urine samples were analyzed by HPLC. Chromatographic separation was performed on a WondaSil C18-WR (150 mm×4.6 mm, 5 μ m) in gradient elution mode using 10.0 mmol/L ammonium acetate aqueous solution (pH 3.90) and acetonitrile as mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 254 nm. Under the optimized conditions, good linearities were obtained in the range of 0.15-300 mg/L, 0.05-100 mg/L, 0.75-750 mg/L, 0.05-100 mg/L, and the detection limits were 1.38×10^-2, 7.67×10^-3, 3.69×10^-2, 1.16×10^-2mg/L for enalapril, triamterene, furosemide and valsartan, respectively. The recoveries were in the range of 89.49%-99.20% with the relative standard deviations (RSDs) among 4.12%-9.44%. The method is simple, accurate and effective, and the results showed the method is applicable for the analysis of the four drugs for kidney diseases in real urine samples.
- high performance liquid chromatography (HPLC),
- enalapril,
- triamterene,
- furosemide,
- valsartan,
- urine,
- kidney disease
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沈阳化工大学材料科学与工程学院沈阳 110142