Citation: QU Baocheng, BIAN Haitao, MAO Xiqin, LI Jin. Measurement of 11 benzophenone ultraviolet-filters in cosmetics by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(12): 1327-1332. doi: 10.3724/SP.J.1123.2015.06042 shu

Measurement of 11 benzophenone ultraviolet-filters in cosmetics by high performance liquid chromatography

1.
Dalian Institute of Food Inspection, Dalian 116630, China

Corresponding author: MAO Xiqin,
Received Date: 24 June 2015

A sample preparation and analytical method with liquid-liquid extraction (LLE) and high performance liquid chromatography (HPLC) was developed to detect 11 benzophenone ultraviolet-filters in cosmetics. The target compounds were extracted by the mixed solutions of tetrahydrofuran (THF)/methanol/water or dichloromethane/water at proper ratios. The extracts were centrifuged and filtered to remove matrix compounds, and then analyzed by HPLC. The separation of analytes was carried out on a Diamonsil-C18 column (150 mm×4.6 mm, 5 μm) with 0.1%(v/v) formic acid aqueous solution (containing 10 mmol/L ammonium acetate) as mobile phase A and methanol containing 0.1%(v/v) formic acid as mobile phase B. The spiked recoveries of the method (n=7) were 93.4%-103.8% with the relative standard deviations of 0.1%-4.2%. The limits of detection (LODs) were in the range of 4.0-30 μg/g and the limits of quantitation (LOQs) ranged from 15 to 100 μg/g. The method was applied to the determination of 42 cosmetic samples randomly purchased from the supermarket in Dalian. Five benzophenone series were always detected, in which the content of benzophenone-3 in sunscreen cream and the content of benzophenone-2 in perfume were very high and reached 2785 μg/g and 2106 μg/g, respectively. The results showed that the developed method is efficient, reliable and sensitive, which can be applied to the determination of benzophenones in cosmetics.
- high performance liquid chromatography (HPLC),
- benzophenone series,
- ultraviolet-filters,
- cosmetics
1. [1]
  [1] Vosough M, Mojdehi N R. Talanta, 2011, 85(4): 2175
2. [2]
  [2] Fent K, Zenker A, Rapp M. Environ Pollut, 2010, 158(5): 1817
3. [3]
  [3] Roman I P, Chisvert A, Canals A. J Chromatogr A, 2011, 1218(18): 2467
4. [4]
  [4] Kameda Y, Kimura K, Miyazaki M. Environ Pollut, 2011, 159(6): 1570
5. [5]
  [5] Kaiser D, Sieratowicz A, Zielke H, et al. Environ Pollut, 2012, 163(4): 84
6. [6]
  [6] Gao L, Yuan T, Wang W H. Asian Journal of Ecotoxicology (高礼, 袁涛, 王文华. 生态毒理学报), 2013, 8(4): 465
7. [7]
  [7] Weisbrod C J, Kunz P Y, Zenker A K, et al. Toxicol Appl Pharm, 2007, 225(3): 255
8. [8]
  [8] Krause M, Klit A, Blomberg Jensen M, et al. Int J Androl, 2012, 35(3): 424
9. [9]
  [9] Zucchi S, Bluthgen N, Ieronimo A, et al. Toxicol Appl Pharm, 2011, 250(2): 137
10. [10]
  [10] Kunz P Y, Fent K. Toxicol Appl Pharm, 2009, 234(1): 77
11. [11]
  [11] Ministry of Health of the People's Republic of China. Hygienic Standard for Cosmetics[2007] (中华人民共和国卫生部. 化妆品卫生规范(2007版)). [2015-06-24]. http://www.Chinacdc.cn/n272442/n272530/n272742/16081.html
12. [12]
  [12] No. 456 Document of the China Food and Drug Administration[2010] (国食药监许[2010] 456号). [2015-06-24]. http://www.sda.gov.cn/WS01/CL0846/56633.html
13. [13]
  [13] Noriya O, Hidehiro H, Rie I, et al. Anal Sci, 2008, 24: 627
14. [14]
  [14] Mao X Q, Bian H T, Qu B C, Chinese Journal of Chromatography (毛希琴, 边海涛, 曲宝成. 色谱), 2013, 31(8): 775
15. [15]
  [15] Barbara K H, Richard M D, Alan J G. Anal Bioanal Chem, 2008, 391: 1293
16. [16]
  [16] Li J, Xu L Y, Xue S W, et al. Chinese Journal of Chromatography (李建, 徐兰英, 薛舒文, 等. 色谱), 2014, 32(10): 1138
17. [17]
  [17] IUPAC. Pure Appl Chem, 1976, 45: 99
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