Citation: BIAN Yingfang, YU Shasha, MOU Renxiang, CAO Zhaoyun, SUN Weihua, YANG Huan, LIN Xiaoyan, CHEN Mingxue. Identification of ustiloxins in false smut balls of rice based on high performance liquid chromatography-high resolution mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(10): 1046-1050. doi: 10.3724/SP.J.1123.2015.04039 shu

Identification of ustiloxins in false smut balls of rice based on high performance liquid chromatography-high resolution mass spectrometry

1.
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Rice, Ministry of Agriculture, China National Rice Research Institute, Hangzhou 310006, China

Corresponding author: CHEN Mingxue,
Received Date: 22 April 2015

Fund Project: 中央级公益性科研院所基本科研业务费专项(2014RG006-3) (2014RG006-3)国家农产品质量安全风险评估专项(GJFP201500701) (GJFP201500701)浙江省科技专项重大农业项目(2014C02002-1). (2014C02002-1)

A sensitive method was developed for the simultaneous identification of five ustiloxins in the false smut balls of rice by high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry (HPLC-LTQ/Orbitrap MS). The samples were extracted with deionized water under ultrasonic condition for 10 min, then purified by a strong cation exchange column (PCX). The ustiloxins were separated on an Xselect HSS T3 column (150 mm×2.1 mm, 3.5 μm) by using 0.1%(v/v) formic acid water solution and methanol as mobile phases with gradient elution at a flow rate of 0.3 mL/min. The full scan range was m/z 200-1000. The confirmatory analysis of the target compounds was carried out by the accurate mass of quasi-molecular ion, isotope abundance ratio and qualitative fragments. The results showed that the five ustiloxins (A, B, C, D and F) were identified from the false smut balls with mass accuracy less than 1×10^-6 (1 ppm) and the absolute values of the deviation of isotope abundance ratio were not more than 3.3%. The product ions were consistent with the theoretical fragment mode. The recoveries were 90% to 105%. This method is accurate and sensitive for the simultaneous identification of the five ustiloxins, which can provide technical means for the research of the ability in toxin producing by Ustilaginodea virens.
- high performance liquid chromatography (HPLC),
- high resolution mass spectrometry (HRMS),
- ustiloxins,
- rice,
- identification
1. [1]
  [1] Ashizawa T, Takahashi M, Moriwaki J, et al. Eur J Plant Pathol, 2010, 128(2): 221
2. [2]
  [2] Jiang S, Tang C S, Tan Z Q. Chinese Journal of Tropical Agriculture (姜慎, 唐春生, 谭志琼. 热带农业科学), 2010, 30(3): 62
3. [3]
  [3] Abbas H K, Shier W T, Cartwright R D, et al. Am J Plant Sci, 2014, 5(21): 3166
4. [4]
  [4] Hu M L, Luo L X, Wang S, et al. Eur J Plant Pathol, 2014, 139(1): 67
5. [5]
  [5] Yang L M, Chen L, Xu J, et al. Journal of Anhui Agricultural University (杨丽敏, 陈莉, 许娟, 等. 安徽农业大学学报), 2012, 39(3): 474
6. [6]
  [6] Yu M N, Yu J J, Hu J K, et al. Fungal Genet Biol, 2015, 76: 10
7. [7]
  [7] Fan Y Q, Zhang S, Yu D Z, et al. Hubei Agricultural Sciences (范允卿, 张舒, 喻大昭, 等. 湖北农业科学), 2012, 51(21): 4701
8. [8]
  [8] Li P X, Evans C D, Wu Y Z, et al. J Am Chem Soc, 2008, 130(7): 2351
9. [9]
  [9] Koiso Y, Li Y, Iwasaki S, et al. J Antibiot, 1994, 47(7): 765
10. [10]
  [10] Fu X X, Wang X H, Cui Y L, et al. Food Chem, 2015, 181: 140
11. [11]
  [11] Yin X L, Chen Z Y, Liu Y F, et al. Scientia Agricultura Sinica (尹小乐, 陈志谊, 刘永锋, 等. 中国农业科学), 2012, 45(22): 4720
12. [12]
  [12] Shan T J, Sun W B, Wang X H, et al. Molecules, 2013, 18(7): 8181
13. [13]
  [13] Ji F, Cao H, Xu J H, et al. Chinese Journal of Rice Science (祭芳, 曹欢, 徐剑宏, 等. 中国水稻科学), 2012, 26(2): 246
14. [14]
  [14] Koiso Y, Natori M, Iwasaki S, et al. Tetrahedron Lett, 1992, 33(29): 4157
15. [15]
  [15] Koiso Y, Morisaki N, Yamashita Y, et al. J Antibiot, 1998, 51(4): 418
16. [16]
  [16] Kong H W, Dai W D, Xu G W. Chinese Journal of Chromatography (孔宏伟, 戴伟东, 许国旺. 色谱), 2014, 32(10): 1052
17. [17]
  [17] Haddad T, Kümmerer K. Chemosphere, 2014, 115: 40
18. [18]
  [18] Llorca M, Rodríguez-Mozaz S, Couillerot O, et al. Chemosphere, 2015, 119: 90
19. [19]
  [19] Umemura M, Nagano N, Koike H, et al. Fungal Genet Biol, 2014, 68: 23
20. [20]
  [20] Isotope distribution calculator and mass spec plotter. [2015-03-12]. http://www.sisweb.com/mstools/isotope.htm
21. [21]
  [21] SANCO/12571/2013
22. [22]
  [22] Varga E, Glauner T, Berthiller F, et al. Anal Bioanal Chem, 2013, 405(15): 5087
23. [23]
  [23] Morisaki N, Koiso Y, Mitsui Y, et al. J Mass Spectrom Soc Jpn, 1998, 46(6): 495
1. [1]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
2. [2]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
3. [3]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
4. [4]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
5. [5]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
6. [6]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
7. [7]
  Tao Yang , Kaijiao Duan , Siyu Li , Jing Wei , Qingdi Yang , Qian Wang . A Comprehensive and Innovative Chemical Experimental Teaching: Extraction and Identification of Tea Polyphenols from Pu'er Tea and the Application in Hand Cream Making. University Chemistry, 2024, 39(8): 270-275. doi: 10.3866/PKU.DXHX202312040
8. [8]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
9. [9]
  Wanqun Hu , Pingping Zhu , Yuan Zheng , Wanqun Zhang , Wei Shao , Hong Wu , Qiang Zhou , Kaiping Yang , Xiang Sheng . Design and Practice of Ideological and Political Case Study in Instrumental Analysis Experiment Course: the Extraction and Structural Identification of Artemisinin. University Chemistry, 2024, 39(2): 203-207. doi: 10.3866/PKU.DXHX202310062
10. [10]
  Shui Hu , Houjin Li , Zhenming Zang , Lianyun Li , Rong Lai . Integration of Science and Education Promotes the Construction of Undergraduate-to-Master’s Integration Experimental Courses: A Case Study on the Extraction, Separation and Identification of Artemisinin from Artemisia annua. University Chemistry, 2024, 39(4): 314-321. doi: 10.3866/PKU.DXHX202310063
11. [11]
  Ziheng Zhuang , Xiao Xu , Kin Shing Chan . Superdrugs for Superbugs. University Chemistry, 2024, 39(9): 128-133. doi: 10.3866/PKU.DXHX202309040
12. [12]
  Yuyang Xu , Ruying Yang , Yanzhe Zhang , Yandong Liu , Keyi Li , Zehui Wei . Research Progress of Aflatoxins Removal by Modern Optical Methods. University Chemistry, 2024, 39(11): 174-181. doi: 10.12461/PKU.DXHX202402064
13. [13]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
14. [14]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
15. [15]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
16. [16]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
17. [17]
  Jiandong Liu , Zhijia Zhang , Mikhail Kamenskii , Filipp Volkov , Svetlana Eliseeva , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-. doi: 10.3866/PKU.WHXB202308048
18. [18]
  Laiying Zhang , Yinghuan Wu , Yazi Yu , Yecheng Xu , Haojie Zhang , Weitai Wu . Innovation and Practice of Polymer Chemistry Experiment Teaching for Non-Polymer Major Students of Chemistry: Taking the Synthesis, Solution Property, Optical Performance and Application of Thermo-Sensitive Polymers as an Example. University Chemistry, 2024, 39(4): 213-220. doi: 10.3866/PKU.DXHX202310126
19. [19]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
20. [20]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(576)
HTML views(117)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142