Citation: ZHENG Penglei, LUO Zhimin, CHANG Ruimiao, GE Yanhui, DU Wei, CHANG Chun, FU Qiang. Preparation of surface molecularly imprinted polymers for penicilloic acid and its adsorption properties[J]. Chinese Journal of Chromatography, ;2015, 33(9): 957-965. doi: 10.3724/SP.J.1123.2015.04036 shu

Preparation of surface molecularly imprinted polymers for penicilloic acid and its adsorption properties

1.
School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China

Corresponding author: FU Qiang,
Received Date: 22 April 2015

Fund Project: 国家自然科学基金项目(81173024) (81173024)国家重大科研仪器设备研制专项基金项目(81227802) (81227802)中国博士后科学基金项目(2014M562428). (2014M562428)

On account of the specificity and reproducibility for the determination of penicilloic acid in penicillin, this study aims to prepare penicilloic acid imprinted polymers (PEOA-MIPs) by surface polymerization method at the surface of modified silica particles by using penicilloic acid (PEOA) as the template molecule, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross linker, and methanol/acetonitrile as the solvents. The synthesis conditions were optimized, and PEOA-MIPs had the best adsorption capacity when the molar ratio of template molecule/functional monomer was 1:4, cross linking degree was 85% and the solvent ratio of methanol/acetonitrile was 1:1 (v/v). The adsorption properties were evaluated by adsorption experiments, including the adsorption isotherms, kinetics and selectivity. The adsorption process between PEOA-MIPs and PEOA fitted the Langmuir adsorption isotherm with the maximum adsorption capacity of 122.78 mg/g and the pseudo-second-order reaction kinetics with fast adsorption kinetics (the equilibrium time of 45 min). The as-synthesized PEOA-MIPs were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and thermal gravimetric analysis (TGA). The results indicated that the MIPs layer has been successfully grafted on the surface of SiO₂ microparticles and the PEOA-MIPs had the excellent thermal stability. The PEOA-MIPs showed the highest selective recognition for PEOA. The PEOA-MIPs possess a high adsorption capacity, rapid mass-transfer rate and high selectivity to PEOA when compared with non-imprinted polymers (PEOA-NIPs). The PEOA-MIPs was expected to be used as the solid phase extraction medium and this study provides the potential applications for fast recognition and analysis of the penicilloic acid in penicillin.
- surface molecularly imprinted polymer,
- penicilloic acid (PEOA),
- molecular recognition,
- adsorption properties
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沈阳化工大学材料科学与工程学院沈阳 110142