Citation: WANG Jue, JIN Yibao, WANG Tiejie, LI Xiaofan. Fingerprint of Solanum nigrum L. from different habitats using high performance liquid chromatography with evaporative light-scattering detection[J]. Chinese Journal of Chromatography, ;2015, 33(8): 809-815. doi: 10.3724/SP.J.1123.2015.04022 shu

Fingerprint of Solanum nigrum L. from different habitats using high performance liquid chromatography with evaporative light-scattering detection

WANG Jue ¹ ,
JIN Yibao ¹ ,
WANG Tiejie ¹ ,
LI Xiaofan ^2,,

1.
1. Shenzhen Institute for Drug Control, Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen 518057, China;

Corresponding author: LI Xiaofan,
Received Date: 14 April 2015

Fund Project: 2015年广东省食品药品监督管理局食品药品检验检测技术创新专项资金资助项目(2015ZX06). (2015ZX06)

Solanum nigrum L. contains a variety of active ingredients. It has been applied broadly in clinical practice. However, the compositions of Solanum nigrum L. from different cultivated areas are quite different. Therefore, it is necessary to set up a standard fingerprint. In this work, a series of Solanum nigrum L. samples were collected from different habitats. A high performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) method was developed to study the differences in quality of these samples. The method was optimized by comparing different columns, gradient or isocratic elution and temperatures of evaporating tube. A Phenomenex C₁₈ column was finally selected for separation with gradient elution. The mobile phases were acetonitrile-20% (v/v) methanol aqueous solution containing 0.03% (v/v) triethylamine. The temperature of evaporating tube was set at 40 ℃. The validation was performed and the results showed that the method had good precision, repeatability and stability. The validated method was applied to detect Solanum nigrum L. from different habitats. The similarities of the acquired chromatograms were analyzed by using the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (TCM). The chromatograms with a similarity between 0.9 and 1.0 were normalized and fitted to form a standard fingerprint of Solanum nigrum L. Thirteen common peaks were found and eleven peaks were identified with authentic standards. The results showed that remarkable differences were found among Solanum nigrum L. from different habitats. These herbs could be distinguished based on the compositions detected by HPLC-ELSD. This method provided a technology to evaluate the homogeneity and stability of Solanum nigrum L with wide application prospects and practical value.
- high performance liquid chromatography (HPLC),
- evaporative light-scattering detection (ELSD),
- Solanum nigrum L.,
- fingerprints
1. [1]
  [1] Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 1. Beijing: China Medical Science Press (国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社), 2000: Appendix 23
2. [2]
  [2] Li Z H, Meng J H, Xiao H. Traditional Chinese Drug Research & Clinical Pharmacology (李志辉, 孟军华, 肖晖. 中药新药与临床药理), 2012, 23(6): 661
3. [3]
  [3] Liu L F, Gao B Y, Gao S H. World Journal of Integrated Traditional and Western Medicine (刘林凤, 高宝益, 高淑红. 世界中西医结合杂志), 2013, 8(2): 144
4. [4]
  [4] Yuan H J, Chen Y G, Cai B C, et al. China Journal of Chinese Materia Medica (袁海建, 陈宜刚, 蔡宝昌, 等. 中国中药杂志), 2011, 36(12): 1630
5. [5]
  [5] Shan H J, Wang B. Chinese Journal of Experimental Traditional Medical Formulae (单会娇, 王冰. 中国实验方剂学杂志), 2010, 16(9): 100
6. [6]
  [6] Luo W J, Wang G H, Zhou X L, et al. Modern Oncology (罗文娟, 王光辉, 周新兰, 等. 现代肿瘤医学), 2007, 15(3): 307
7. [7]
  [7] Loganayaki N, Siddhuraju P, Manian S. Food Sci Biotechnol, 2010, 19(1): 121
8. [8]
  [8] Wang H C, Chung P J, Wu C H, et al. J Sci Food Agric, 2011, 91(1): 178
9. [9]
  [9] Shan H J, Zhang J K, Xu L, et al. Chinese Traditional Patent Medicine (单会娇, 张建逵, 许亮, 等. 中成药), 2011, 33(3): 483
10. [10]
  [10] WHO. Guidelines for the Assessment of Herbal Medicines. Geneva, 1996: 178
11. [11]
  [11] Center for Drug Evaluation and Research (CDER) of Food and Drug Administration (FDA). Guidance for Industry-Botanical Drug Products (Draft Guidance). Rockville, 2004
12. [12]
  [12] Sun G X, Dou X W, Yang L P, et al. Chinese Journal of Chromatography (孙国祥, 豆小文, 杨兰萍, 等. 色谱), 2013, 31(5): 456
13. [13]
  [13] Zhou X L. [PhD Dissertation]. Shenyang: Shenyang Pharmaceutical University (周新兰. [博士学位论文]. 沈阳: 沈阳药科大学), 2006
14. [14]
  [14] Luo D, Luo G A, Chen L, et al. Pharmacy and Clinics of Chinese Materia Medica (罗丹, 罗国安, 陈丽, 等. 中药与临床), 2014, 5(6): 21
15. [15]
  [15] Ding H L, Li C, Jin P, et al. Chinese Journal of Chromatography (丁洪流, 李灿, 金萍, 等. 色谱), 2013, 31(8): 804
16. [16]
  [16] Zhao H, Wang Y H, Liu F, et al. Chinese Journal of Chromatography (赵卉, 王玉红, 刘芳, 等. 色谱), 2013, 31(8): 795
1. [1]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
2. [2]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
3. [3]
  Jia-He Li , Yu-Ze Liu , Jia-Hui Ma , Qing-Xiao Tong , Jian-Ji Zhong , Jing-Xin Jian . 洛芬碱衍生物的合成、化学发光与重金属离子检测. University Chemistry, 2025, 40(6): 230-237. doi: 10.12461/PKU.DXHX202407080
4. [4]
  Cun WANG , Shaohan XU , Yuqian ZHANG , Yaoyao ZHANG , Tao GONG , Rong WEN , Yuhang LIAO , Yanrong REN . Terbium complex electrochemiluminescent emitters: Synthesis and application in the detection of epinephrine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1351-1360. doi: 10.11862/CJIC.20240427
5. [5]
  Pengli GUAN , Renhu BAI , Xiuling SUN , Bin LIU . Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1817-1826. doi: 10.11862/CJIC.20250058
6. [6]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
7. [7]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
8. [8]
  Hui Zhang , Zijian Zhao , Yajing Wang , Kai Ni , Yanfei Wang , Liang Zhu , Jianyun Liu , Xiaoyu Zhao . Structurally engineered solvent-free LiFePO₄ electrodes via hot-pressing with efficient ion transport pathways for lithium extraction from brine. Acta Physico-Chimica Sinica, 2026, 42(2): 100130-0. doi: 10.1016/j.actphy.2025.100130
9. [9]
  Weina Wang , Fengyi Liu , Wenliang Wang . “Extracting Commonality, Delving into Typicals, Deriving Individuality”: Constructing a Knowledge Graph of Crystal Structures. University Chemistry, 2024, 39(3): 36-42. doi: 10.3866/PKU.DXHX202308029
10. [10]
  Peipei Zhu , Li Wang , Lili Chen , Xunfan Liao , Yiwang Chen . Intelligent Teaching in Analytical Chemistry: An Exploration Based on Knowledge Graphs. University Chemistry, 2025, 40(10): 23-32. doi: 10.12461/PKU.DXHX202411073
11. [11]
  Hongxia Li , Yu Li , Jie Xin , Xuan Wang . Practice of College Chemistry Curriculum Reform Based on Course Knowledge Graph. University Chemistry, 2025, 40(11): 141-149. doi: 10.12461/PKU.DXHX202504031
12. [12]
  Ruiqin Feng , Ye Fan , Yun Fang , Yongmei Xia . Strategy for Regulating Surface Protrusion of Gold Nanoflowers and Their Surface-Enhanced Raman Scattering. Acta Physico-Chimica Sinica, 2024, 40(4): 2304020-0. doi: 10.3866/PKU.WHXB202304020
13. [13]
  Zijun Huang , Feng Wu , Shaofeng Pi , Saijin Huang , Zhengjun Fang . Knowledge Graph-based Development of AI Curriculum for Inorganic Chemistry Experiments and Exploration of New Teaching Paradigm. University Chemistry, 2025, 40(9): 228-237. doi: 10.12461/PKU.DXHX202504052
14. [14]
  Kun Rong , Cuilian Wen , Jiansen Wen , Xiong Li , Qiugang Liao , Siqing Yan , Chao Xu , Xiaoliang Zhang , Baisheng Sa , Zhimei Sun . Hierarchical MoS₂/Ti₃C₂T_x heterostructure with excellent photothermal conversion performance for solar-driven vapor generation. Acta Physico-Chimica Sinica, 2025, 41(6): 100053-0. doi: 10.1016/j.actphy.2025.100053
15. [15]
  Wenliang Wang , Weina Wang , Sufan Wang , Tian Sheng , Tao Zhou , Nan Wei . “Schrödinger Equation – Approximate Models – Core Concepts – Simple Applications”: Constructing a Logical Framework and Knowledge Graph of Atom and Molecule Structures. University Chemistry, 2024, 39(8): 338-343. doi: 10.3866/PKU.DXHX202312084
16. [16]
  Ling Li , Guocheng Wang . 知识图谱与AI助教在无机化学混合式教学中的初步探索——以“沉淀溶解平衡”的教学为例. University Chemistry, 2025, 40(6): 1-8. doi: 10.12461/PKU.DXHX202407063
17. [17]
  Weigang Zhu , Xiaofei Ma , Yun Tian , Huaji Liu , Fanli Lu , Yalu Ma . 基于知识图谱的“无机化学与化学分析”课程信息化教学资源构建与应用研究. University Chemistry, 2025, 40(6): 9-15. doi: 10.12461/PKU.DXHX202408113
18. [18]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
19. [19]
  Minna Ma , Yujin Ouyang , Yuan Wu , Mingwei Yuan , Lijuan Yang . Green Synthesis of Medical Chemiluminescence Reagents by Photocatalytic Oxidation. University Chemistry, 2024, 39(5): 134-143. doi: 10.3866/PKU.DXHX202310093
20. [20]
  Qin Li , Kexin Yang , Qinglin Yang , Xiangjin Zhu , Xiaole Han , Tao Huang . Illuminating Chlorophyll: Innovative Chemistry Popularization Experiment. University Chemistry, 2024, 39(9): 359-368. doi: 10.3866/PKU.DXHX202309059

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(571)
HTML views(13)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142