Citation: WANG Shuo, ZHANG Xiangming, ZHANG Jing, SHAO Bing, LI Shuming. Determination of 49 drugs and 5 metabolites in drinking water samples using ultra-high performance liquid chromatography- electrospray ionization tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(7): 730-739. doi: 10.3724/SP.J.1123.2015.03004 shu

Determination of 49 drugs and 5 metabolites in drinking water samples using ultra-high performance liquid chromatography- electrospray ionization tandem mass spectrometry

1.
1. Chaoyang District Center for the Disease Control and Prevention, Beijing 100021, China;

Corresponding author: LI Shuming,
Received Date: 16 March 2015

Fund Project: 首都卫生行业发展专项(2011-7023-02). (2011-7023-02)

A method for the determination of 54 drugs in drinking water samples was developed by using ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI MS/MS). The target drugs in drinking water samples were enriched and cleaned-up by HLB solid-phase extraction (SPE) cartridges and then eluted with 5 mL methanol. The elute was collected, concentrated under a gentle stream of nitrogen gas, diluted with 0.4 mL 0.1% formic acid solution, and analyzed by UPLC-ESI MS/MS. The separation of the 54 drugs was performed on an ACQUITY UPLC^TMBEH C₁₈ column using mobile phases of 0.1% formic acid and methanol by gradient elution. The multiple reaction monitoring (MRM) mode was employed in mass spectrometry acquisition. The matrix-matched external standard calibration was used for quantitation. The results showed that the average recoveries of the drugs in ground water, tap water and surface water were 58.7%-104.4%, 53.1%-109.5%, and 50.7%-118.8%, respectively, and the corresponding relative standard deviations (RSD, n=6) were 0.3%-12.8%, 1.0%-15.5%, and 0.4%-19.3%, respectively. The method quantification limits (MQL) for target compounds were in the range of 0.002-5.000 ng/L. The developed method was applied to analyze the water samples from Beijing. The results showed that 26 drugs were detected in ground water samples.
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沈阳化工大学材料科学与工程学院沈阳 110142