Citation: LI Xin, ZHANG Yaoqin, AI Lianfeng, WANG Xuesheng, WANG Manman, XU Houjun, HAO Yulan. Determination of five avermectins in bovine liver by on-line solid-phase extraction with hydrophobic monolithic column coupled with high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(6): 590-596. doi: 10.3724/SP.J.1123.2015.02062 shu

Determination of five avermectins in bovine liver by on-line solid-phase extraction with hydrophobic monolithic column coupled with high performance liquid chromatography-tandem mass spectrometry

1.
1. School of Public Health, North China University of Science and Technology, Tangshan 063000, China;

Corresponding author: WANG Xuesheng,
Received Date: 20 March 2015

Fund Project: 国家自然科学基金项目(21305028) (21305028)河北省自然科学基金项目(B2013209238) (B2013209238)河北省高等学校科学研究计划项目(Q2012155) (Q2012155)国家质检总局项目(2013IK154,2014IK092). (2013IK154,2014IK092)

A method based on on-line solid-phase extraction (SPE) with hydrophobic monolithic column coupled with high performance liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of five avermectins in bovine liver. A poly(butyl methacrylate-co-ethylene dimethacrylate) monolithic column was used as the sorbent. The parameters influenced on on-line SPE and separation process such as the loading mobile phase, the eluting flow rate and the solvent for the separation were investigated in detail. Blank samples, spiked samples, matrix effect and recovery experiments were investigated to evaluate the extraction efficiency and potential interfering compounds originating from the matrix. Under the optimized conditions, the method showed a linear range of 1-100 μg/L and the quantification limit of 5 μg/kg for each analyte. The presented method gave recoveries of 77.4%-98.4%. The relative standard deviations of intra-day and inter-day were 4.46%-8.03% and 4.79%-8.68%, respectively. Moreover, no significant changes were found in the extraction performance after more than 400 usages on one monolithic column, and even on the monoliths with various batches. The feasibility of the developed poly(butyl methacrylate-co-ethylene dimethacrylate) monolithic column based on the on-line SPE method for the determination of avermectins was further demonstrated by the analysis of real samples.
1. [1]
  [1] Moreira N, Bernardi M M, Spinosa H S. Neurotoxicol Teratol, 2014, 43: 33
2. [2]
  [2] Cao Y, Deng W, Li Y P, et al. Chinese Journal of Pesticide Science (曹源, 邓维, 李永平, 等. 农药学学报), 2014, 16(1): 54
3. [3]
  [3] Merola V M, Eubig P A. Vet Clin North Am Small Anim Pract, 2012, 42(2): 313
4. [4]
  [4] Chukwudebe A C, Beavers J B, Jaber M, et al. Environ Toxicol Chem, 1998, 17(6): 1118
5. [5]
  [5] Rubensam G, Barreto F, Hoff R B, et al. Food Contr, 2013, 29(1): 55
6. [6]
  [6] European Food Safety Authority. EFSA J, 2014, 12(9): 3823
7. [7]
  [7] GB 28260-2011
8. [8]
  [8] GB/T 21320-2007
9. [9]
  [9] Pozo O J, Marin J M, Sancho J V, et al. J Chromatogr A, 2003, 992: 133
10. [10]
  [10] Tao Y F, Yu G, Chen D M, et al. J Chromatogr B, 2012, 897: 64
11. [11]
  [11] Piestansky J, Marakova K, Veizerova L, et al. Anal Chim Acta, 2014, 826: 84
12. [12]
  [12] Liu R Z, Ruan T, Wang T, et al. Talanta, 2014, 120: 158
13. [13]
  [13] Yang X T, Hu Y F, Li G K. J Chromatogr A, 2014, 1342: 37
14. [14]
  [14] Ai L F, Ma Y S, Chen R C, et al. Chinese Journal of Analytical Chemistry (艾连峰, 马育松, 陈瑞春, 等. 分析化学), 2013, 41(8): 1194
15. [15]
  [15] Zhang Z B, Ou J J, Lin H, et al. Chemical Journal of Chinese Universities (张振宾, 欧俊杰, 林辉, 等. 高等学校化学学报), 2013, 34(9): 2011
16. [16]
  [16] Zhang C J, Pan J L, Zhang Z M, et al. Chinese Journal of Chromatography (张成江, 潘加亮, 张卓旻, 等. 色谱), 2014, 32(10): 1034
17. [17]
  [17] Namera A, Saito T. TrAC-Trend Anal Chem, 2013, 45: 182
18. [18]
  [18] Wei Y, Chen X, Yang S C, et al. Chinese Journal of Analytical Chemistry (韦誉, 陈霞, 杨胜超, 等. 分析化学), 2014, 42(4): 495
19. [19]
  [19] Zhang L, Wang H. Journal of Instrumental Analysis (张立, 王海. 分析测试学报), 2014, 33(7): 739
20. [20]
  [20] Sun L, Lu Y, Yang T, et al. Chinese Journal of Analysis Laboratory (孙蕾, 鹿毅, 杨涛, 等. 分析试验室), 2014, 33(7): 791
21. [21]
  [21] Liu Y, Wang M M, Ai L F, et al. J Sep Sci, 2014, 37(13): 1648
22. [22]
  [22] Zheng H J, Liu Q W, Jia Q. J Chromatogr A, 2014, 1343: 47
23. [23]
  [23] Xu Y, Xu P Z, Cao Q, et al. Chinese Journal of Analytical Chemistry (徐溢, 徐平洲, 曹强, 等. 分析化学), 2008, 36(12): 1636
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
4. [4]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
5. [5]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
6. [6]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
7. [7]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065
8. [8]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
9. [9]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
10. [10]
  Haiyang Zhang , Yanzhao Dong , Haojie Li , Ruili Guo , Zhicheng Zhang , Jiangjiexing Wu . Exploring the Integration of Chemical Engineering Principle Experiment with Cutting-Edge Research Achievements. University Chemistry, 2024, 39(10): 308-313. doi: 10.12461/PKU.DXHX202405035
11. [11]
  Ziheng Zhuang , Xiao Xu , Kin Shing Chan . Superdrugs for Superbugs. University Chemistry, 2024, 39(9): 128-133. doi: 10.3866/PKU.DXHX202309040
12. [12]
  Lihui Jiang , Wanrong Dong , Hua Yang , Yongqing Xia , Hongjian Peng , Jun Yuan , Xiaoqian Hu , Zihan Zeng , Yingping Zou , Yiming Luo . Study on Extraction of p-Hydroxyacetophenone. University Chemistry, 2024, 39(11): 259-268. doi: 10.12461/PKU.DXHX202402056
13. [13]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
14. [14]
  Xiyuan Su , Zhenlin Hu , Ye Fan , Xianyuan Liu , Xianyong Lu . Change as You Want: Multi-Responsive Superhydrophobic Intelligent Actuation Material. University Chemistry, 2024, 39(5): 228-237. doi: 10.3866/PKU.DXHX202311059
15. [15]
  Cunming Yu , Dongliang Tian , Jing Chen , Qinglin Yang , Kesong Liu , Lei Jiang . Chemistry “101 Program” Synthetic Chemistry Experiment Course Construction: Synthesis and Properties of Bioinspired Superhydrophobic Functional Materials. University Chemistry, 2024, 39(10): 101-106. doi: 10.12461/PKU.DXHX202408008
16. [16]
  Rong Lai , Jie Li , Xianfang Xu , Shui Hu , Tao Chen , Houjin Li , Guping Hu , Hongyan Chen , Fang Zhu . Taking the Overall Relocation as an Opportunity to Accelerate the Development of Chemical Experimental Teaching Centers: A Case Study of the National Demonstration Center for Experimental Chemistry Education at Sun Yat-Sen University. University Chemistry, 2024, 39(4): 33-39. doi: 10.3866/PKU.DXHX202310115
17. [17]
  Yaping ZHANG , Tongchen WU , Yun ZHENG , Bizhou LIN . Z-scheme heterojunction β-Bi₂O₃ pillared CoAl layered double hydroxide nanohybrid: Fabrication and photocatalytic degradation property. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 531-539. doi: 10.11862/CJIC.20240256
18. [18]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
19. [19]
  Qiuyang LUO , Xiaoning TANG , Shu XIA , Junnan LIU , Xingfu YANG , Jie LEI . Application of a densely hydrophobic copper metal layer in-situ prepared with organic solvents for protecting zinc anodes. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1243-1253. doi: 10.11862/CJIC.20240110
20. [20]
  Yangrui Xu , Yewei Ren , Xinlin Liu , Hongping Li , Ziyang Lu . 具有高传质和亲和表面的NH₂-UIO-66基疏水多孔液体用于增强CO₂光还原. Acta Physico-Chimica Sinica, 2024, 40(11): 2403032-. doi: 10.3866/PKU.WHXB202403032

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(366)
HTML views(58)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142