Citation: YU Lu, SONG Wei, LÜ Yaning, ZHAO Muyu, ZHOU Fangfang, HU Yanyun, ZHENG Ping. Rapid determination of 204 pesticide residues in tea by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(6): 597-612. doi: 10.3724/SP.J.1123.2015.02056 shu

Rapid determination of 204 pesticide residues in tea by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

YU Lu ^1,2 ,
SONG Wei ^2,3 ,
LÜ Yaning ^2,3 ,
ZHAO Muyu ^1,2 ,
ZHOU Fangfang ^1,2 ,
HU Yanyun ^2,3 ,
ZHENG Ping ^2,3,,

1.
1. College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China;

Corresponding author: ZHENG Ping,
Received Date: 5 February 2015

Fund Project: 安徽省自然科学基金项目(1408085MKL96,1408085MKL97) (1408085MKL96,1408085MKL97)国家质检总局科研课题(AHCIQ_2014IK130). (AHCIQ_2014IK130)

An analytical method was established for the simultaneous determination of 204 pesticide residues in tea by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS). The samples were extracted with acetonitrile, and cleaned-up by solid phase extraction (SPE) with a Carb-PSA cartridge, eluted with acetonitrile-toluene (3:1, v/v), determined by UPLC-Q-TOF/MS and quantified by external standard method. A data base of the accurate mass numbers and a library which contains the 204 pesticides were established. The automatic retrieval of detection results was carried on according to the characteristics of the compound, such as accurate mass, retention time, isotopic ratio, and so on. Based on the above results, the qualitative identifications of the 204 pesticides were accomplished without the contrast of standard substances. The results indicated that this method can be used to determine the 204 pesticides in tea. At the three spiked levels of 10, 20, 50 μg/kg, mean recoveries for the 204 pesticides in tea were between 68.1% and 117.2%, with the relative standard deviations (RSDs) ranging from 3.1% to 18.9%. The limits of quantification for the 204 pesticides were lower than 10 μg/kg. The method has been applied to four positive samples, and the results generally accord with the detection results by the method of GB/T 23205-2008. This method has the characteristics of high efficiency, as well as high sensitivity and accuracy, which can meet the requirement of the determination of the 204 pesticides in tea.
- ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS),
- pesticides,
- multi-residues,
- tea
1. [1]
  [1] Ma H M, Wang Y Q, Qian H. China Tea Processing (马惠民, 王永强, 钱和. 中国茶叶加工), 2012(4): 18
2. [2]
  [2] Li Y. [MS Dissertation]. Qinhuangdao: Yanshan University (李岩. [硕士学位论文]. 秦皇岛: 燕山大学), 2013
3. [3]
  [3] GB 2763-2014
4. [4]
  [4] Dong J B, Wang J H. Food Science (董金斌, 王金花. 食品科学), 2009, 30(12): 230
5. [5]
  [5] Deng X J, Qian G, Chen X P, et al. Food Chem, 2014, 145: 853
6. [6]
  [6] Mezcua M, Martinez-Uroz A, Fernandez-Alba A. Gas Chromatography. London: Elsevier, 2012
7. [7]
  [7] Liu B, Zhao H X. Chinese Journal of Analysis Laboratory (刘冰, 赵红霞. 分析试验室), 2013, 32(10): 77
8. [8]
  [8] Zhao H X, Zhao S C, Deng L G, et al. Food Anal Methods, 2013, 6: 497
9. [9]
  [9] Jia W, Huang J R, Ling Y, et al. Journal of Instrumental Analysis (贾玮, 黄峻榕, 凌云, 等. 分析测试学报), 2013, 32(1): 9
10. [10]
  [10] Pang G F, Fan C L, Li Y, et al. Journal of Instrumental Analysis (庞国芳, 范春林, 李岩, 等. 分析测试学报), 2012, 31(9): 1017
11. [11]
  [11] GB/T 23205-2008
12. [12]
  [12] Li X Y, Zhang H Y, Fan C L, et al. Chemistry (李晓颖, 张红医, 范春林, 等. 化学通报), 2014, 77(2): 123
13. [13]
  [13] Peng X, Zhao Z Y, Kang J, et al. Chinese Journal of Analysis Laboratory (彭兴, 赵志远, 康健, 等. 分析试验室), 2014, 33(3): 282
14. [14]
  [14] Li X J, Peng T, Li C J. Journal of Instrumental Analysis (李晓娟, 彭涛, 李重九. 分析测试学报), 2012, 31(5): 628
15. [15]
  [15] Zhao Z Y, Shi Z H, Kang J, et al. Chinese Journal of Chromatography (赵志远, 石志红, 康健, 等. 色谱), 2013, 31(4): 372
16. [16]
  [16] Mezcua M, Malato O, Garcia-Reyes J F, et al. Anal Chem, 2009, 81(3): 913
17. [17]
  [17] Zhang F, Yu C T, Wang W W, et al. Anal Chim Acta, 2012, 757: 39
18. [18]
  [18] Lou Z Y, Chen Z M, Luo F J, et al. Chinese Journal of Chromatography (楼正云, 陈宗懋, 罗逢健, 等. 色谱), 2008, 26(5): 568
19. [19]
  [19] JAP-178 GC/MS
20. [20]
  [20] Hou R Y, Bian H Z, Zhao X X, et al. Journal of Instrumental Analysis (侯如燕, 卞红正, 赵秀霞, 等. 分析测试学报), 2011, 30(1): 58
21. [21]
  [21] Lou Z Y, Tang F B, Chen Z M, et al. Chinese Journal of Analysis Laboratory (楼正云, 汤富彬, 陈宗懋, 等. 分析试验室), 2009, 28(Suppl): 76
22. [22]
  [22] Xu J, Chen J, Ye H Y, et al. Journal of Instrumental Analysis (徐娟, 陈捷, 叶弘毅, 等. 分析测试学报), 2011, 30(9): 990
23. [23]
  [23] Feng Y W, Xue Q H, Wang Z J. Food and Fermentation Industries (冯永巍, 薛庆海, 汪振炯. 食品与发酵工业), 2013, 39(5): 176
24. [24]
  [24] 2002/657/EC
25. [25]
  [25] Xiang P, Shen M, Zhuo X Y. Journal of Instrumental Analysis (向平, 沈敏, 卓先义. 分析测试学报), 2009, 28(6): 753
26. [26]
  [26] Duan J Y. [MS Dissertation]. Beijing: Chinese Academy of Agricultural Sciences (段俊彦. [硕士学位论文]. 北京: 中国农业科学院), 2009
1. [1]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064
4. [4]
  Xiyuan Su , Zhenlin Hu , Ye Fan , Xianyuan Liu , Xianyong Lu . Change as You Want: Multi-Responsive Superhydrophobic Intelligent Actuation Material. University Chemistry, 2024, 39(5): 228-237. doi: 10.3866/PKU.DXHX202311059
5. [5]
  Zongpei Zhang , Yanyang Li , Yanan Si , Kai Li , Shuangquan Zang . Developing a Chemistry Experiment Center Employing a Multifaceted Approach to Serve High-Quality Laboratory Education. University Chemistry, 2024, 39(7): 13-19. doi: 10.12461/PKU.DXHX202404041
6. [6]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023
7. [7]
  Xingyuan Lu , Yutao Yao , Junjing Gu , Peifeng Su . Energy Decomposition Analysis and Its Application in the Many-Body Effect of Water Clusters. University Chemistry, 2025, 40(3): 100-107. doi: 10.12461/PKU.DXHX202405074
8. [8]
  Liang TANG , Jingfei NI , Kang XIAO , Xiangmei LIU . Synthesis and X-ray imaging application of lanthanide-organic complex-based scintillators. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1892-1902. doi: 10.11862/CJIC.20240139
9. [9]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
10. [10]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357
11. [11]
  Conghao Shi , Ranran Wang , Juli Jiang , Leyong Wang . The Illustration on Stereoisomers of Macrocycles Containing Multiple Chiral Centers via Tröger Base-based Macrocycles. University Chemistry, 2024, 39(7): 394-397. doi: 10.3866/PKU.DXHX202311034
12. [12]
  Pengcheng Yan , Peng Wang , Jing Huang , Zhao Mo , Li Xu , Yun Chen , Yu Zhang , Zhichong Qi , Hui Xu , Henan Li . Engineering Multiple Optimization Strategy on Bismuth Oxyhalide Photoactive Materials for Efficient Photoelectrochemical Applications. Acta Physico-Chimica Sinica, 2025, 41(2): 100014-. doi: 10.3866/PKU.WHXB202309047
13. [13]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
14. [14]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
15. [15]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
16. [16]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
17. [17]
  Ruoxi Sun , Yiqian Xu , Shaoru Rong , Chunmiao Han , Hui Xu . The Enchanting Collision of Light and Time Magic: Exploring the Footprints of Long Afterglow Lifetime. University Chemistry, 2024, 39(5): 90-97. doi: 10.3866/PKU.DXHX202310001
18. [18]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
19. [19]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
20. [20]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(421)
HTML views(95)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142