Citation: ZHANG Xieguang, ZHENG Yanjie, ZENG Yongting, LIU Wenli. Direct analysis of 38 polyphenols in wine by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(6): 583-589. doi: 10.3724/SP.J.1123.2015.01040 shu

Direct analysis of 38 polyphenols in wine by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry

1.
Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518109, China

Corresponding author: ZHANG Xieguang,
Received Date: 28 January 2015

Fund Project: 深圳市技术攻关项目(JSGG20140519114050315). (JSGG20140519114050315)

Wine has been described previously as a rich source of polyphenols. However, an accurate screening of its complete phenolic profile is still lacking. In the present work, the analysis of 38 polyphenols in wine using ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry (UPLC-LTQ/Orbitrap MS) was explored. Wines were directly detected. The sample was loaded onto a Thermo Hypersil Gold C18 column (100 mm×2.1 mm, 1.9 μm) using a gradient elution of acetonitrile/water containing 0.1% (v/v) formic acid for the separation. UPLC-LTQ/Orbitrap mass spectrometer acquired full scan MS date for quantification, and data dependent MS² product ion spectra for identification and/or confirmation. The regression coefficients (R²) for the calibration curves (two orders of magnitude up to the lowest calibration level) in the study were ≥0.99. The limits of detection for the 38 compounds were 0.002-0.50 mg/kg. The average recoveries at three spiked levels were in the range of 90%-102% with the relative standard deviations (RSDs) of 0.51%-2.56%. Mass errors were always ≤ 5 ppm. This procedure was then successfully applied to the analysis of the polyphenols in wines.
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沈阳化工大学材料科学与工程学院沈阳 110142