Citation: JIN Zuyao, LÜ Yayao, ZHOU Shanshan, HAO Feiran, FU Bin, YING Wantao, QIAN Xiaohong, ZHANG Yangjun. A simple preparation method of an electric heating apparatus for heating capillary chromatographic columns and its application in liquid chromatography-mass spectrometry system[J]. Chinese Journal of Chromatography, ;2015, 33(6): 563-570. doi: 10.3724/SP.J.1123.2015.01036 shu

A simple preparation method of an electric heating apparatus for heating capillary chromatographic columns and its application in liquid chromatography-mass spectrometry system

JIN Zuyao ^1,2 ,
LÜ Yayao ² ,
ZHOU Shanshan ² ,
HAO Feiran ² ,
FU Bin ² ,
YING Wantao ² ,
QIAN Xiaohong ² ,
ZHANG Yangjun ^1,2,,

1.
1. Anhui Medical University, Hefei 230032, China;

Corresponding author: ZHANG Yangjun,
Received Date: 27 January 2015

Fund Project: 国家重大科学计划项目(2012CB910603) (2012CB910603)国家高技术研究发展规划项目(2012AA020202) (2012AA020202)国家重大科学仪器设备开发专项项目(2011YQ09000504,2012YQ12004407,2011YQ030139,2011YQ06008408) (2011YQ09000504,2012YQ12004407,2011YQ030139,2011YQ06008408)国家自然科学基金项目(21275519,20735005,31100591). (21275519,20735005,31100591)

For deep coverage of proteome, especially in performing qualitative identification and quantitative analysis of low-abundance proteins, the most commonly used method is the application of a longer capillary chromatographic column or a capillary column packed with smaller particle sizes. However, this causes another problem, the very high back pressure which results in liquid leaks in some connection parts in a liquid chromatograph. To solve this problem, an electric heating apparatus was developed to raise the temperature of a capillary column for reducing its back pressure, which was further applied in a capillary high performance liquid chromatography-tandem mass spectrometry system (cHPLC-MS/MS), and evaluated in the terms of chromatographic column back pressure and chromatographic column efficiency using bovine serum albumin (BSA) tryptic digests and yeast tryptic digests, separately. The results showed that at the optimum current, our electric heating apparatus could reduce the column pressure of a capillary column packed with 3 μm packing materials by at least 50% during the separation of BSA tryptic digestion and yeast tryptic digestion, compared with that without electric heating. The column efficiency was also increased slightly. This suggested that the electric heating apparatus can significantly reduce the column pressure, which provides an efficient way to use capillary chromatographic columns packed with smaller sizes of particles at a lower pressure.
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