Citation: ZHENG Xiangping, DING Liping, CHEN Zhitao, GUO Jing, ZHANG Rui, WU Wenfan. Rapid determination of thiabendazole and carbendazim in concentrated fruit juices by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(6): 652-656. doi: 10.3724/SP.J.1123.2015.01034 shu

Rapid determination of thiabendazole and carbendazim in concentrated fruit juices by ultra-high performance liquid chromatography-tandem mass spectrometry

1.
Fuqing Entry-Exit Inspection and Quarantine Bureau, Fuqing 350300, China

Corresponding author: ZHENG Xiangping,
Received Date: 22 January 2015

Fund Project: 福建省教育厅科技项目(JK2012023). (JK2012023)

A method was developed for the rapid determination of thiabendazole and carbendazim residues in concentrated fruit juices by using ultra-high performance liquid chromatography (UHPLC) coupled with triple quadrupole tandem mass spectrometry (MS/MS), and quantified by matrix-matched standard solution in external standard method. The residues in the samples were extracted by ethyl acetate, and then analyzed by using UHPLC-MS/MS in multiple reaction monitoring (MRM) mode via positive electrospray ionization with an Waters ACQUITY UPLC BEH C18 column (50 mm×2.1 mm, 1.7 μm) as the analytical column. Good linearities were obtained in the range of 0.5-10 μg/kg for both pesticides with correlation coefficients greater than 0.99. The recovery experiments were carried out by spiking standards into blank samples of apple, peach, orange, pear, grape juices at three levels of 0.5, 1.0 and 5.0 μg/kg. The recoveries of thiabendazole and carbendazim were from 76.98% to 108.7% with the relative standard deviations (RSD) of 2.95%-9.99%. For both pesticides in different matrices, the limits of detection (S/N=3) were in the range of 0.12-0.23 μg/kg. Meanwhile, the pyrolysis mechanism and matrix effects for the determination of thiabendazole and carbendazim in concentrated fruit juices were investigated in this study. The method is simple, rapid and accurate, and can be used for the routine analysis of thiabendazole and carbendazim in concentrated fruit juices.
- ultra-high performance liquid chromatography (UPLC),
- tandem mass spectrometry (MS/MS),
- thiabendazole,
- carbendazim,
- concentrated fruit juices
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沈阳化工大学材料科学与工程学院沈阳 110142