Citation: CAO Xinyue, PANG Guofang, JIN Linghe, KANG Jian, HU Xueyan, CHANG Qiaoying, WANG Minglin, FAN Chunlin. Comparison of the performances of gas chromatography- quadrupole time of flight mass spectrometry and gas chromatography-tandem mass spectrometry in rapid screening and confirmation of 208 pesticide residues in fruits and vegetables[J]. Chinese Journal of Chromatography, ;2015, 33(4): 389-396. doi: 10.3724/SP.J.1123.2014.12026 shu

Comparison of the performances of gas chromatography- quadrupole time of flight mass spectrometry and gas chromatography-tandem mass spectrometry in rapid screening and confirmation of 208 pesticide residues in fruits and vegetables

CAO Xinyue ^1,2 ,
PANG Guofang ² ,
JIN Linghe ^1,2 ,
KANG Jian ^2,3 ,
HU Xueyan ² ,
CHANG Qiaoying ^2,3 ,
WANG Minglin ^1,, ,
FAN Chunlin ^2,,

1.
1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;

Corresponding author: WANG Minglin, FAN Chunlin,
Received Date: 18 December 2014
Available Online: 19 January 2015
Fund Project: "十二五"国家科技支撑项目(2012BAD29B01). (2012BAD29B01)

The performances of gas chromatography-tandem mass spectrometry (GC-MS/MS) and gas chromatography quadrupole time of flight mass spectrometry (GC-QTOF/MS) for the determination of 208 pesticide residues in fruit and vegetable samples, including apple, orange, tomato and cucumber, were compared comprehensively. Based on the differences of the two instruments, their respective characteristics and scopes of application in the detection of the pesticide residues were presented, which provided the reference for the analysis of pesticide residues. The performance parameters of the two instruments, such as overall recoveries, precisions, limits of detection, linear ranges, identification points and matrix effects, were evaluated according to a designed experiment. At three spiked levels (5.0, 10.0 and 20.0 μg/kg), the average recoveries for the majority of pesticides (93.0%) ranged from 70% to 120% in the four matrices with relative standard deviations below 20%. The limits of detection for most of the pesticides by GC-MS/MS and GC-Q-TOF/MS were less than 5.0 μg/kg. Compared with GC-QTOF/MS, GC-MS/MS showed relatively lower limits of detection and wider linear ranges, and its performance was more satisfactory in accurate quantitative analysis due to its superior sensitivity. On the other hand, GC-QTOF/MS provided accurate mass measurement, which was proved to be an efficient analytical tool on the rapid screening and confirmation of a large number of pesticides and non-target compounds.
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