Citation: BAI Haihong, FAN Chao, SHEN Bingquan, TIAN Fang, DENG Yulin, PAN Yiting, QIN Weijie, QIAN Xiaohong. Synthesis of core-shell hydrophilic polymer-silica hybrid material and its application in N-glycan enrichment[J]. Chinese Journal of Chromatography, ;2015, 33(3): 221-227. doi: 10.3724/SP.J.1123.2014.11022 shu

Synthesis of core-shell hydrophilic polymer-silica hybrid material and its application in N-glycan enrichment

BAI Haihong ^1,2 ,
FAN Chao ² ,
SHEN Bingquan ^1,2 ,
TIAN Fang ² ,
DENG Yulin ¹ ,
PAN Yiting ^3,, ,
QIN Weijie ^2,, ,
QIAN Xiaohong ²

1.
1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China;

Corresponding author: PAN Yiting, QIN Weijie,
Received Date: 16 November 2014
Available Online: 11 December 2014
Fund Project: 国家重大科学计划项目(2013CB911204) (2013CB911204)国家重大科学仪器设备开发专项项目(2011YQ09000504) (2011YQ09000504)国家自然科学基金项目(21275005,21235001). (21275005,21235001)

Protein N-glycosylation is one of the most important post-translational modifications closely correlated with many important biological and pathological processes. The structural alterations of N-linked glycans in glycoproteins are always associated with many diseases, such as diabetes, heart failure and malignant tumors. Therefore, it is very important to establish sensitive methods for high-throughput N-glycan profiling. However, the low abundance of the N-glycoproteins and the heterogeneity of the N-glycans make it a challenge to analyse the protein glycosylation sensitively. In this work, we had synthesized core-shell hydrophilic polymer-silica hybrid materials (pGMAG-SiO₂) for the efficient enrichment of protein N-glycans. Firstly, pGMAG-SiO₂ was prepared by in situ growth of glucose polymer on the surface of silica microparticles using surface-initiated atom transfer radical polymerization (SI-ATRP) technique. The strong hydrophilicity of the material makes it suitable for the enrichment of N-glycans released from complex samples. Secondly, maltoheptaose and the N-glycans from chicken egg albumin were used as standard samples to optimize the enrichment conditions and evaluate the enrichment efficiency of pGMAG-SiO₂. Finally, pGMAG-SiO₂ was applied to the enrichment of N-linked glycans from human plasma proteins and 47 glycoforms were successfully identified after enrichment. These results demonstrated the high enrichment efficiency and significant application value of pGMAG-SiO₂ in the analysis of N-glycans.
- surface-initiated atom transfer radical polymerization (SI-ATRP),
- core-shell silica packing,
- N-glycan,
- N-glycoprotein
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