Citation: LEI Meikang, PENG Fang, DING Tao, ZHU Zitong, XU Jiawen, WU Xiaoqin. Determination of wilforine in honey using solid phase extraction purification and ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(1): 65-67. doi: 10.3724/SP.J.1123.2014.08022 shu

Determination of wilforine in honey using solid phase extraction purification and ultra performance liquid chromatography-tandem mass spectrometry

LEI Meikang ^1,, ,
PENG Fang ¹ ,
DING Tao ² ,
ZHU Zitong ¹ ,
XU Jiawen ¹ ,
WU Xiaoqin ¹

1.
1. Technology Center of Quzhou Entry-Exit Inspection and Quarantine Bureau, Quzhou 324002, China;

Corresponding author: LEI Meikang,
Received Date: 22 August 2014
Available Online: 23 September 2014
Fund Project: 浙江省公益性技术应用研究计划项目(2013C37101). (2013C37101)

A method based on solid phase extraction and ultra performance liquid chromatography coupled with tandem mass spectrometry (SPE-UPLC-MS/MS) has been proposed for the determination of wilforine residue in honey. After the sample was dissolved with water, concentrated and purified by an HLB solid phase extraction cartridge, the UPLC separation was performed on a Hypersil GOLD C18 column (50 mm ×2.1 mm, 1.9 μm) utilizing a gradient elution program of methanol (containing 0.15% formic acid) and water as mobile phases at a flow rate of 0.25 mL/min. The determination was carried out with electrospray ion source in the positive mode (ESI⁺) and multiple reaction monitoring (MRM) mode. The mass concentration of wilforine in the range of 0.01-2 μg/L was linearly correlated with the peak area, and the correlation coefficients was greater than 0.998. The limit of quantification (S/N>10) for wilforine was 0.01 μg/kg. The recoveries were 76.1% to 96.2% in the spiked levels of 0.01, 0.05 and 0.5 μg/kg with the relative standard deviations (RSD, n=6) lower than 10%. The results indicate that the method is rapid, sensitive and accurate, and can be applied for the qualitative and quantitative analysis of wilforine in honey.
- solid phase extraction (SPE),
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- wilforine,
- honey
1. [1]
  [1] Chen X H, Cai M Q, Jin M C. Chinese Journal of Clinical Pharmacy (陈晓红, 蔡美强, 金米聪. 中国临床药学杂志), 2011, 20(2): 73
2. [2]
  [2] Huang Y Y, Liu H, Liu F, et al. Journal of Chinese Medicinal Materials (黄云英, 刘虹, 刘芳, 等. 中药材), 2012, 35(5): 739
3. [3]
  [3] Zhang C H, Wu H Q, Huang X L, et al. Chinese Journal of Analytical Chemistry (张春华, 吴惠勤, 黄晓兰, 等. 分析化学), 2012, 40(6): 862
4. [4]
  [4] Fang W F. Apiculture of China (方文富. 中国蜂业), 2007, 58(3): 24
5. [5]
  [5] Xuan Y, Fu D F, Sun N, et al. Physical Testing and Chemical Analysis Part B: Chemical Analysis (宣宇, 傅得锋, 孙楠, 等. 理化检验: 化学分册), 2012, 48(7): 777
6. [6]
  [6] Yan J H. Deep Processing of Bee Products and Formulation Technology. Beijing: Chinese Agricultural Science and Technology Press (闫继红. 蜂产品深加工与配方技术. 北京: 中国农业科学技术出版社), 2005: 65
7. [7]
  [7] Jing Z X. Apiculture of China (荆战星. 中国蜂业), 2011, 62(11): 43
8. [8]
  [8] Yao H C, Yao J H, Chen Y. Journal of Bee (姚海春, 姚京辉, 陈云. 蜜蜂杂志), 2012(12): 34
9. [9]
  [9] GB 14963-2011
10. [10]
  [10] Hu Z N, Cheng D, Dai Y L, et al. Chinese Journal of Pesticide Science (胡兆农, 程丹, 戴亚丽, 等. 农药学学报), 2008, 10(1): 75
11. [11]
  [11] GB/T 23408-2009
12. [12]
  [12] Gao Y Y, Zhang Z H, Liu X, et al. Chinese Journal of Chromatography (高洋洋, 张朝晖, 刘鑫, 等. 色谱), 2014, 32(5): 524
13. [13]
  [13] Wang F, Li T, Ma C. Chinese Journal of Chromatography (王菲, 李彤, 马辰. 色谱), 2013, 31(3): 191
14. [14]
  [14] GB/T 27404-2008
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
4. [4]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
5. [5]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
6. [6]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
7. [7]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065
8. [8]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
9. [9]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
10. [10]
  Haiyang Zhang , Yanzhao Dong , Haojie Li , Ruili Guo , Zhicheng Zhang , Jiangjiexing Wu . Exploring the Integration of Chemical Engineering Principle Experiment with Cutting-Edge Research Achievements. University Chemistry, 2024, 39(10): 308-313. doi: 10.12461/PKU.DXHX202405035
11. [11]
  Lihui Jiang , Wanrong Dong , Hua Yang , Yongqing Xia , Hongjian Peng , Jun Yuan , Xiaoqian Hu , Zihan Zeng , Yingping Zou , Yiming Luo . Study on Extraction of p-Hydroxyacetophenone. University Chemistry, 2024, 39(11): 259-268. doi: 10.12461/PKU.DXHX202402056
12. [12]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
13. [13]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
14. [14]
  Yingying Chen , Di Xu , Congmin Wang . Exploration and Practice of the “Four-Level, Three-Linkage” General Chemistry Course System. University Chemistry, 2024, 39(8): 119-125. doi: 10.3866/PKU.DXHX202401057
15. [15]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
16. [16]
  Limei CHEN , Mengfei ZHAO , Lin CHEN , Ding LI , Wei LI , Weiye HAN , Hongbin WANG . Preparation and performance of paraffin/alkali modified diatomite/expanded graphite composite phase change thermal storage material. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 533-543. doi: 10.11862/CJIC.20230312
17. [17]
  Zhilian Liu , Wengui Wang , Hongxiao Yang , Yu Cui , Shoufeng Wang . Ideological and Political Education Design for the Synthesis of Irinotecan Drug Intermediate 7-Ethyl Camptothecin. University Chemistry, 2024, 39(2): 89-93. doi: 10.3866/PKU.DXHX202306012
18. [18]
  Jing WU , Puzhen HUI , Huilin ZHENG , Pingchuan YUAN , Chunfei WANG , Hui WANG , Xiaoxia GU . Synthesis, crystal structures, and antitumor activities of transition metal complexes incorporating a naphthol-aldehyde Schiff base ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2422-2428. doi: 10.11862/CJIC.20240278
19. [19]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
20. [20]
  Zhaomei LIU , Wenshi ZHONG , Jiaxin LI , Gengshen HU . Preparation of nitrogen-doped porous carbons with ultra-high surface areas for high-performance supercapacitors. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 677-685. doi: 10.11862/CJIC.20230404

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(239)
HTML views(6)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142