Citation: GUO Shoudong, SANG Hui, YANG Nana, KAN Yujie, LI Fuyu, LI Yu, LI Fangyuan, QIN Shucun. Measurement of sialic acid from lipoproteins and human plasma by liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(11): 1197-1200. doi: 10.3724/SP.J.1123.2014.07002 shu

Measurement of sialic acid from lipoproteins and human plasma by liquid chromatography-tandem mass spectrometry

1.
Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University, Taian 271000, China

Corresponding author: QIN Shucun,
Received Date: 2 July 2014
Available Online: 14 August 2014
Fund Project: 国家自然科学基金(31300639,81170785) (31300639,81170785)山东省青年基金项目(ZR2013HQ014) (ZR2013HQ014)山东省中医药科学技术研究项目(2013-257) (2013-257)泰山医学院博士启动基金项目(2234). (2234)

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established to quantify sialic acid (N-acetylneuraminic acid, NANA) from lipoproteins and human plasma. The method was used to investigate the different contents of NANA from lipoproteins between diabetic with an average age of 51.6 years and healthy participants with an average age of 50.7 years. The NANA from lipoprotein samples was hydrolyzed by acetic acid (pH=2) at 80 ℃ for 2 h and analyzed by the optimized LC-MS/MS method after high speed centrifugation and filtration. The limits of detection and quantification of NANA were 7.4 and 24.5 pg, respectively. The linear range was 2.5-80 ng/mL for NANA and the correlation coefficient (R²) was more than 0.998. The levels of NANA in the plasma of type Ⅱ diabetics and healthy participants were (548.3±88.9) and (415.3±55.5) mg/L, respectively; and the levels of NANA from very low density lipoproteins (VLDL), low density lipoproteins (LDL), and high density lipoproteins (HDL) of the type Ⅱ diabetics and the healthy participants were (4.91±0.19), (6.95±0.28), (3.61±0.22) μg/mg and (2.90±0.27), (7.03±0.04), (2.40±0.09) μg/mg, respectively. The sialic acid levels of VLDL and HDL from the type Ⅱ diabetics were markedly higher than those of the corresponding healthy participants with the similar ages (P<0.01). The method can rapidly determine the sialic acid from lipoproteins, and is reproducible and time saving.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- sialic acid,
- lipoprotein,
- human plasma,
- diabetes mellitus
1. [1]
  [1] Dodani S, Grice D G, Joshi S. J Clin Lipidol, 2009, 3(2): 70
2. [2]
  [2] Millar J S, Anber V, Shepherd J, et al. Atherosclerosis, 1999, 145: 253
3. [3]
  [3] Cong P X, Li Z J, Xu J, et al. Chinese Journal of Chromatography (丛培旭, 李兆杰, 徐杰, 等. 色谱), 2013, 31(5): 399
4. [4]
  [4] Camejo G, López A, López F, et al. Atherosclerosis, 1985, 55: 93
5. [5]
  [5] Stratton P D, Lumb P J, Paganga G, et al. Atherosclerosis, 2001, 154: 285
6. [6]
  [6] Gavella M, Lipovac V, Car A, et al. Acta Diabetol, 2003, 40: 95
7. [7]
  [7] Serdar Z, Yesilbursa D, Dirican M, et al. Cell Biochem Funct, 2007, 25: 655
8. [8]
  [8] Anumula K R. Anal Biochem, 1995, 230: 24
9. [9]
  [9] Hikita T, Tadano-Aritomi K, Iida-Tanaka N, et al. Anal Biochem, 2000, 281: 193
10. [10]
  [10] Reuter G, Pfeil R, Stoll S, et al. Eur J Biochem, 1983, 134: 139
11. [11]
  [11] Ortner K, Buchberger W. Electrophoresis, 2008, 29(10): 2233
12. [12]
  [12] Rajendiran S, Lakshamanappa H S, Zachariah B, et al. Am J Trop Med Hyg, 2008, 79(3): 372
13. [13]
  [13] Kontush A, John Chapman M. Pharmacol Rev, 2006, 58: 342
14. [14]
  [14] Phillips C, Owens D, Collins P, et al. Atherosclerosis, 2005, 181: 109
15. [15]
  [15] Iijima R, Takahashi H, Namme R, et al. FEBS Lett, 2004, 561: 163
16. [16]
  [16] Ogasawara Y, Namai T, Yoshino F, et al. FEBS Lett, 2007, 581(13): 2473
17. [17]
  [17] Lindbohm N, Gylling H, Miettinen T A. J Lipid Res, 2000, 41: 1110
18. [18]
  [18] Filipovic I, Schwarzmann G, Mraz W, et al. Eur J Biochem, 1979, 93: 51
19. [19]
  [19] Xie H L, Li C, Liu N. Chinese Journal of Chromatography (解鸿蕾, 李春, 刘宁. 色谱), 2013, 31(8): 781
20. [20]
  [20] Hou X C, Zhu L P, Liu C S, et al. Modern Food Science and Technology (侯向昶, 朱丽萍, 刘春生, 等. 现代食品科技), 2013, 29(7): 1706
1. [1]
  Sirui Xin , Jiayin Zhou , Kin Shing Chan . Smelling Disease: E-nose. University Chemistry, 2024, 39(9): 141-145. doi: 10.3866/PKU.DXHX202309051
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
5. [5]
  Hongling Liu , Yue Xia , Guang Xu , Yafei Yang , Chunhua Qu . Bitter Cold Medicine, Good for Healing. University Chemistry, 2025, 40(3): 328-332. doi: 10.12461/PKU.DXHX202405039
6. [6]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
7. [7]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
8. [8]
  Yiming Lu , Xiang Xie , Xiaoqing Qiu , Yang Liu , Xinyuan Cheng . The New Year’s Eve of the Aviation Brake Material Family. University Chemistry, 2024, 39(9): 203-207. doi: 10.12461/PKU.DXHX202403061
9. [9]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
10. [10]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
11. [11]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
12. [12]
  Yan Li , Fei Ding , Jing Wang , Jing Nan , Yijun Li , Xiaohang Qiu . Give a Man a Fish, and Teach a Man to Fish: Self-Designed Instrumental Analysis Experiments and Integration of Ideological and Political Elements. University Chemistry, 2024, 39(2): 208-213. doi: 10.3866/PKU.DXHX202310097
13. [13]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
14. [14]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
15. [15]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
16. [16]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
17. [17]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
18. [18]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032
19. [19]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
20. [20]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(623)
HTML views(63)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142