Citation: LI Ruiping, YUAN Qin, HUANG Yingping. Hydrophilic interaction chromatography on silica column:retention mechanism and its influential factors[J]. Chinese Journal of Chromatography, ;2014, 32(7): 675-681. doi: 10.3724/SP.J.1123.2014.04048 shu

Hydrophilic interaction chromatography on silica column:retention mechanism and its influential factors

LI Ruiping ^1,2 ,
YUAN Qin ¹ ,
HUANG Yingping ^1,2,,

1.
1. Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang 443002, China;

Corresponding author: HUANG Yingping,
Received Date: 29 April 2014
Available Online: 29 May 2014
Fund Project: 国家自然科学基金项目(20977058) (20977058)国家水体污染控制与治理科技重大专项(2012Zxo7104-002-02). (2012Zxo7104-002-02)

Hydrophilic interaction chromatography (HILIC) is a valuable alternative to reversed phase liquid chromatography (RPLC) for the analysis of highly polar and hydrophilic compounds, in which the separation mechanism is quite different from RPLC and the separation selectivity is complementary to RPLC. This separation mode can be characterized as normal phase liquid chromatography (NPLC) on polar columns in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). Silica has been the earliest developed and most widely used HILIC stationary phase. This review deals with the recent advances in the development of the retention mechanism on silica column with special attention to the effects of stationary phase, mobile phase composition and temperature on separation in HILIC mode. Moreover, the developing trends and applications of this HILIC mode are presented.
- hydrophilic interaction chromatography (HILIC),
- retention mechanism,
- silica column,
- mobile phase composition,
- column temperature
1. [1]
  [1] Alpert A J. J Chromatogr, 1990, 499: 177
2. [2]
  [2] Melnikov S M, Höltzel A, Seidel-Morgenstern A, et al. Anal Chem, 2013, 85: 8850
3. [3]
  [3] Gama M R, da Costa Silva R G, Collins C H, et al. Trend Anal Chem, 2012, 37: 48
4. [4]
  [4] Buszewski B, Noga S. Anal Bioanal Chem, 2012, 402: 231
5. [5]
  [5] Jandera P. Anal Chim Acta, 2011, 692: 1
6. [6]
  [6] Nguyen H P, Schug K A. J Sep Sci, 2008, 31: 1465
7. [7]
  [7] Periat A, Boccard J, Veuthey J-L, et al. J Chromatogr A, 2013, 1312: 49
8. [8]
  [8] Periat A, Grand-Guillaume Perrenoud A, Guillarme D. J Sep Sci, 2013, 36: 3141
9. [9]
  [9] Soukup J, Janás P, Jandera P. J Chromatogr A, 2013, 1286: 111
10. [10]
  [10] Louw S, Pereira A S, Lynen F, et al. J Chromatogr A, 2008, 1208: 90
11. [11]
  [11] Ikegami T, Tomomatsu K, Takubo H, et al. J Chromatogr A, 2008, 1184: 474
12. [12]
  [12] Heaton J C, Wang X, Barber W E, et al. J Chromatogr A, 2014, 1328: 7
13. [13]
  [13] Mazzarino M, Fiacco I, de la Torre X, et al. J Chromatogr A, 2011, 1218: 8156
14. [14]
  [14] Nemoto T, Lee X-P, Kumazawa T, et al. J Pharm Biomed Anal, 2014, 88: 71
15. [15]
  [15] Zhou G, Pang H, Tang Y, et al. Food Chem, 2014, 150: 260
16. [16]
  [16] Gokmen V, Serpen A, Mogol B A. Anal Bioanal Chem, 2012, 403: 2915
17. [17]
  [17] Nováková L, Gottvald T, Vlčková H, et al. J Chromatogr A, 2012, 1259: 237
18. [18]
  [18] Periat A, Debrus B, Rudaz S, et al. J Chromatogr A, 2013, 1282: 72
19. [19]
  [19] Nováková L. J Chromatogr A, 2013, 1292: 25
20. [20]
  [20] Hemström P, Irgum K. J Sep Sci, 2006, 29: 1784
21. [21]
  [21] Guo Y, Gaiki S. J Chromatogr A, 2011, 1218: 5920
22. [22]
  [22] Li R P, Huang J X. Progress in Chemistry (李瑞萍, 黄骏雄. 化学进展), 2006, 18(11): 1508
23. [23]
  [23] Olsen B A, Pack B W. Hydrophilic Interaction Chromatography: A Guide for Practitioners. New Jersey: John Wiley & Sons, Inc., 2013
24. [24]
  [24] García-Gómez D, Rodríguez-Gonzalo E, Carabias-Martínez R. Trend Anal Chem, 2013, 47: 111
25. [25]
  [25] Naidong W. J Chromatogr B, 2003, 796: 209
26. [26]
  [26] Guo Y L, Yuan Q, Li R P, et al. Chinese Journal of Chromatography (郭亚丽, 袁琴, 李瑞萍, 等. 色谱), 2012, 30(3): 232
27. [27]
  [27] Bernal J, Ares A M, Pól J, et al. J Chromatogr A, 2011, 1218: 7438
28. [28]
  [28] van Nuijs A L N, Tarcomnicu I, Covaci A. J Chromatogr A, 2011, 1218: 5964
29. [29]
  [29] Li R P, Guo Y L, Yuan Q. J Liq Chromatogr Rel Technol, 2011, 34: 1112
30. [30]
  [30] Jian W Y, Edom R W, Xu Y, et al. J Sep Sci, 2010, 33: 681
31. [31]
  [31] Ares A M, Bernal J. Cent Eur J Chem, 2012, 10(3): 534
32. [32]
  [32] Kahsay G, Song H, Schepdael A V, et al. J Pharm Biomed Anal, 2014, 87: 142
33. [33]
  [33] Dejaegher B, Heyden Y V. J Sep Sci, 2010, 33: 698
34. [34]
  [34] Spagou K, Tsoukali H, Raikos N, et al. J Sep Sci, 2010, 33: 716
35. [35]
  [35] Shen A J, Guo Z M, Liang X M. Progress in Chemistry (沈爱金, 郭志谋, 梁鑫淼. 化学进展), 2014, 26(1): 10
36. [36]
  [36] McCalley D V. J Chromatogr A, 2007, 1171: 46
37. [37]
  [37] EPA Method 1694: Pharmaceuticals and Personal Care Products in Water, Soil, Sediment, and Biosolids by HPLC/MS/MS. [2014-05-20]. http://water.epa.gov/scitech/methods/cwa/bioindicators/upload/2008_01_03_methods_method_1694.pdf
38. [38]
  [38] Ferrer I, Zweigenbaum J A, Thurman E M, et al. J Chromatogr A, 2010, 1217: 5674
39. [39]
  [39] Soukup J, Janás P, Jandera P. J Chromatogr A, 2013, 1286: 111
40. [40]
  [40] Heaton J C, Russell J J, Underwood T, et al. J Chromatogr A, 2014, 1347: 39
41. [41]
  [41] Pesek J J, Matyska M T, Boysen R I, et al. Trend Anal Chem, 2013, 42: 64
42. [42]
  [42] Quintela O, Lendoiro E, Cruz A, et al. Anal Bioanal Chem, 2010, 396: 1703
43. [43]
  [43] Wu X, Huang W, Lu L, et al. Anal Bioanal Chem, 2010, 398: 1319
44. [44]
  [44] Peng L, Jiang T, Rong Z, et al. J Chromatogr B, 2011, 879: 3927
45. [45]
  [45] Wu X, Zhu B, Lu L, et al. Food Chem, 2012, 133: 482
46. [46]
  [46] Luo H, Zhang L, Xue F, et al. Food Anal Methods, 2014, 7: 308
47. [47]
  [47] Chauve B, Guillarme D, Cléon P, et al. J Sep Sci, 2010, 33: 752
48. [48]
  [48] Grumbach E S, Diehl D M, Neue U D. J Sep Sci, 2008, 31: 1511
49. [49]
  [49] Orentiene A, Olsauskaite V, Vickackaite V, et al. J Chromatogr A, 2011, 1218: 6884
50. [50]
  [50] Backe W J, Yingling V, Johnson T. J Chromatogr A, 2014, 1334: 72
51. [51]
  [51] Bidlingmeyer B A, Del Rios J K, Korpi J. Anal Chem, 1982, 54: 442
52. [52]
  [52] Law B, Gill R, Moffat A C. J Chromatogr, 1984, 301: 165
53. [53]
  [53] Cox G B, Stout R W. J Chromatogr, 1987, 384: 315
54. [54]
  [54] McCalley D V, Neue U D. J Chromatogr A, 2008, 1192: 225
55. [55]
  [55] Kumar A, Heaton J C, McCalley D V. J Chromatogr A, 2013, 1276: 33
56. [56]
  [56] McKeown A P, Euerby M R, Lomax H, et al. J Sep Sci, 2001, 24: 835
57. [57]
  [57] Li R P, Huang J X. J Chromatogr A, 2004, 1041: 163
58. [58]
  [58] Li R P, Zhang Y, Lee C C, et al. J Chromatogr A, 2010, 1217: 1799
59. [59]
  [59] McCalley D V. J Chromatogr A, 2010, 1217: 3408
60. [60]
  [60] Jin G, Guo Z, Zhang F, et al. Talanta, 2008, 76: 522
61. [61]
  [61] Li R P. Journal of China Three Gorges University (李瑞萍. 三峡大学学报), 2005, 27(1): 76
62. [62]
  [62] Olsen B A. J Chromatogr A, 2001, 913: 113
63. [63]
  [63] McCalley D V. J Chromatogr A, 2008, 1193: 85
64. [64]
  [64] Fountain K J, Xu J, Diehl D M, et al. J Sep Sci, 2010, 33: 740
65. [65]
  [65] Damme T V, Lachová M, Lynen F, et al. Anal Bioanal Chem, 2014, 406: 401
66. [66]
  [66] dos Santos Pereira A, Girón A J, Admasu E, et al. J Sep Sci, 2010, 33: 834
67. [67]
  [67] Gaber Y, Törnvall U, Kumar M A, et al. Green Chem, 2011, 13: 2021
68. [68]
  [68] dos Santos Pereira A, David F, Vanhoenacker G, et al. J Sep Sci, 2009, 32: 2001
69. [69]
  [69] Hayama T, Yoshida H, Todoroki K, et al. Rapid Commun Mass Spectrom, 2008, 22: 2203
70. [70]
  [70] Gritti F, dos Santos Pereira A, Sandra P, et al. J Chromatogr A, 2009, 1216: 8496
71. [71]
  [71] Gritti F, dos Santos Pereira A, Sandra P, et al. J Chromatogr A, 2010, 1217: 683
72. [72]
  [72] Dong L L, Huang J X. Chromatographia, 2007, 65: 519
73. [73]
  [73] Bicker W, Wu J Y, Lmmerhofer M, et al. J Sep Sci, 2008, 31: 2971
74. [74]
  [74] Hao Z, Xiao B, Weng N. J Sep Sci, 2008, 31: 1449
75. [75]
  [75] Shou W Z, Naidong W. J Chromatogr B, 2005, 825: 186
76. [76]
  [76] Liu M, Chen E X, Ji R, et al. J Chromatogr A, 2008, 1188: 255
77. [77]
  [77] Li R P, Yuan Q, Zhang Y, et al. J Liq Chromatogr Rel Technol, 2011, 34: 511
78. [78]
  [78] Guo Y, Gaiki S. J Chromatogr A, 2005, 1074: 71
79. [79]
  [79] Chirita R-I, West C, Finaru A-L, et al. J Chromatogr A, 2010, 1217: 3091
80. [80]
  [80] Wang P G, He W. Hydrophilic Interaction Chromatography (HILIC) and Advanced Applications. New York: CRC Press, 2011
81. [81]
  [81] Guo Y, Srinivasan S, Gaiki S. Chromatographia, 2007, 66: 223
82. [82]
  [82] Lundanes E, Greibrokk T. Adv Chromatogr, 2006, 44: 45
83. [83]
  [83] Zhu P L, Dolan J W, Snyder L R, et al. J Chromatogr A, 1996, 756: 51
84. [84]
  [84] Louw S, Lynen F, Hanna-Brown M, et al. J Chromatogr A, 2010, 1217: 514
1. [1]
  Gengjia Chen , Junjie Ou . Application of the van Deemter Equation in Instrumental Analysis Teaching: A Case of Organic Polymer Monolithic Columns. University Chemistry, 2025, 40(11): 362-368. doi: 10.12461/PKU.DXHX202502003
2. [2]
  Yaping ZHANG , Tongchen WU , Yun ZHENG , Bizhou LIN . Z-scheme heterojunction β-Bi₂O₃ pillared CoAl layered double hydroxide nanohybrid: Fabrication and photocatalytic degradation property. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 531-539. doi: 10.11862/CJIC.20240256
3. [3]
  Xiaojing Tian , Zhichun Huang , Qingsong Zhang , Xu Wang , Ning Yang , Nanping Deng . PNIPAm Thermo-Responsive Nanofibers Mats: Morphological Stability and Response Behavior under Cross-Linking. Acta Physico-Chimica Sinica, 2024, 40(4): 2304037-0. doi: 10.3866/PKU.WHXB202304037
4. [4]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
5. [5]
  Weina Wang , Lixia Feng , Fengyi Liu , Wenliang Wang . Computational Chemistry Experiments in Facilitating the Study of Organic Reaction Mechanism: A Case Study of Electrophilic Addition of HCl to Asymmetric Alkenes. University Chemistry, 2025, 40(3): 206-214. doi: 10.12461/PKU.DXHX202407022
6. [6]
  Xudong Liu , Huili Fan , Junping Xiao , Min Yang , Yan Li . Teaching Approaches to the A_E + A_N Mechanism of Electrophilic Addition Reactions between Olefins and Inorganic Acids in Organic Chemistry. University Chemistry, 2025, 40(7): 367-372. doi: 10.12461/PKU.DXHX202409041
7. [7]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
8. [8]
  Qiang Wu , Wenhua Hou . Teaching Classical Contents Newly: Taking Temperature–Entropy Diagram as an Example. University Chemistry, 2025, 40(4): 399-407. doi: 10.12461/PKU.DXHX202407102
9. [9]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
10. [10]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
11. [11]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
12. [12]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
13. [13]
  Weilai Yu , Chuanbiao Bie . Unveiling S-Scheme Charge Transfer Mechanism. Acta Physico-Chimica Sinica, 2024, 40(4): 2307022-0. doi: 10.3866/PKU.WHXB202307022
14. [14]
  Chi Zhang , Yi Xu , Xiaopeng Guo , Zian Jie , Ling Li . 五彩斑斓的秘密——物质显色机理. University Chemistry, 2025, 40(6): 266-275. doi: 10.12461/PKU.DXHX202407061
15. [15]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
16. [16]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
17. [17]
  Xiaosong PU , Hangkai WU , Taohong LI , Huijuan LI , Shouqing LIU , Yuanbo HUANG , Xuemei LI . Adsorption performance and removal mechanism of Cd(Ⅱ) in water by magnesium modified carbon foam. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1537-1548. doi: 10.11862/CJIC.20240030
18. [18]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
19. [19]
  Jiajie Li , Xiaocong Ma , Jufang Zheng , Qiang Wan , Xiaoshun Zhou , Yahao Wang . Recent Advances in In-Situ Raman Spectroscopy for Investigating Electrocatalytic Organic Reaction Mechanisms. University Chemistry, 2025, 40(4): 261-276. doi: 10.12461/PKU.DXHX202406117
20. [20]
  Rohit Kumar , Anita Sudhaik , Aftab Asalam Pawaz Khan , Van Huy Neguyen , Archana Singh , Pardeep Singh , Sourbh Thakur , Pankaj Raizada . Designing tandem S-scheme photo-catalytic systems: Mechanistic insights, characterization techniques, and applications. Acta Physico-Chimica Sinica, 2025, 41(11): 100150-0. doi: 10.1016/j.actphy.2025.100150

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(403)
HTML views(20)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142