Citation: LI Yan, XU Jilin, ZHENG Liyang, LI Min, YAN Xiaojun, LUO Qijun. Simultaneous determination of ten phytohormones in five parts of Sargasum fusiforme (Hary.) Seichell by high performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(8): 861-866. doi: 10.3724/SP.J.1123.2014.03049 shu

Simultaneous determination of ten phytohormones in five parts of Sargasum fusiforme (Hary.) Seichell by high performance liquid chromatography-triple quadrupole mass spectrometry

1.
School of Marine Sciences, Ningbo University, Ningbo 315211, China

Corresponding author: LUO Qijun,
Received Date: 28 March 2014
Available Online: 20 May 2014
Fund Project: 东海区优势种类扩繁及高效健康养殖技术集成与示范项目(2011BAD13B08). (2011BAD13B08)

A method for the simultaneous determination of indole-3-acetic acid, N₆-(2-isopentenyl) adenosine, N₆-(2-isopentenyl) adenine, trans-zeatin riboside, zeatin, strigolactone, abscisic acid, salicylic acid, gibberellin A3 and jasmonic acid in five different parts of main branch, lateral branch, primary leaf, secondary leaf and stem of Sargasum fusiforme (Hary.) Seichell was established by high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-TQMS). The samples were extracted with methanol/water/formic acid (15:4:1, v/v/v)(containing 0.5% 2,6-di-tert-butyl-4-methylphenol, BHT) after vacuum freeze-drying. The separation was performed on a Hypersil Gold C18 column by using methanol and water as mobile phases with gradient elution. The analytes were detected by tandem mass spectrometry in selected reaction monitoring (SRM) mode. The electrospray ionization (ESI) source was used for the quantitative analysis in the positive mode or negative mode. Under the optimized conditions, the correlation coefficients (r) of the ten phytohormones were from 0.9989 to 1.0000 in the linear ranges. The detection limits of the ten phytohormones were 0.0012-4.6512 μg/L. The average recoveries were 72.24%-91.31% with the relative standard deviations not more than 6.59%. In the five parts of fresh Sargasum fusiforme (Hary.) Seichell samples, the contents of the ten phytohormones were from not detected to 4041.431 ng/g. This method has good sensitivity, precision, recovery, and can be used to simultaneously determine the phytohormones.
1. [1]
  [1] Luan Z W, Dong F, Ji Y B. Heilongjiang Medicine Journal (栾志伟, 东方, 季宇彬, 等. 黑龙江医药), 2013, 26(4): 592
2. [2]
  [2] Wang W, Lu J B, Wang C, et al. Inte J Biolog Macromole, 2013, 58: 127
3. [3]
  [3] Xie H J, Ye H X, Shen T, et al. Journal of Zhejiang Agricultural Sciences (谢何杰, 叶慧娴, 沈婷, 等. 浙江农业科学), 2014(4): 487
4. [4]
  [4] Yu Z Q, Shuai L, Li S Y, et al. Journal of Medical Research (于竹芹, 帅莉, 李生尧, 等. 医学研究杂志), 2011, 40(8): 43
5. [5]
  [5] Si Y Y, Yu X Y, Li S B, et al. Guangdong Agricultural Sciences (司圆圆, 余祥勇, 李双波, 等. 广东农业科学), 2013, 40(4): 144
6. [6]
  [6] Zhang S S, Cai Z Y, Wang X L. PNAS, 2009, 106(11): 4543
7. [7]
  [7] Santner A, Calderon-Villalobos L I A, Estelle M. Nat Chem Biol, 2009, 5(5): 301
8. [8]
  [8] Li Y N, Wu H L, Zhu S H, et al. Anal Sci, 2009, 25: 83
9. [9]
  [9] Zhang L, Guan H L, He Z K. Science China: Chemistry, 2010, 53(1): 245
10. [10]
  [10] Ji Y B, Wang C, Wu T, et al. Chinese Journal of Chromatography (季宇彬, 王翀, 吴涛, 等. 色谱), 2007, 25(3): 322
11. [11]
  [11] Li Q, Wang R Z, Huang Z G, et al. Chinese Chemical Letters, 2010, 21: 472
12. [12]
  [12] Liu Z Y, Shen C Z, Dong Y Y. Chemistry and Bioengineering (刘志勇, 沈春章, 董元彦. 化学与生物工程), 2006, 23(2): 55
13. [13]
  [13] Zhou Y M, Xin X. Food Science (周艳明, 忻雪. 食品科学), 2010, 31(18): 301
14. [14]
  [14] Zhang Y Q, Zhong Y L, Gao C Y, et al. Chinese Journal of Chromatography (张玉琼, 仲延龙, 高翠云, 等. 色谱), 2013, 31(8): 800
15. [15]
  [15] Durgbanshi A, Arbona V, Pozo O, et al. J Agric Food Chem, 2005, 53: 8437
16. [16]
  [16] Chiwocha S D S, Cutler A J, Abrams S R, et al. Plant J, 2005, 42: 35
17. [17]
  [17] Pan X, Welti R, Wang X. Phytochemistry, 2008, 69: 1773
18. [18]
  [18] Xie H B, Zhou M Y, Zhao H F, et al. Chinese Journal of Chromatography (谢寒冰, 周明莹, 赵海峰, 等. 色谱), 2014, 32(5): 493
19. [19]
  [19] Kojima M, Kamada-Nobusada T, Komatsu H, et al. Plant Cell Physiol, 2009, 50(7): 1201
20. [20]
  [20] Wu Q, Wang L, Wu D P, et al. Chinese Journal of Chromatography (吴倩, 王璐, 吴大朋, 等. 色谱), 2014, 32(4): 319
21. [21]
  [21] Olsson J, Claeson K, Karlberg B, et al. J Chromatogr A, 1998, 824: 231
22. [22]
  [22] Rameau C. C R Biologies, 2010, 333: 344
23. [23]
  [23] Gomez-Roldan V, Fermas S, Brewer P B, et al. Nature, 2008, 455: 189
24. [24]
  [24] Umehara M, Hanada A, Yoshida S, et al. Nature, 2008, 455: 195
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沈阳化工大学材料科学与工程学院沈阳 110142