Citation: MA Hui, SONG Shaojiang, LIU Feifei, ZHANG Yan, PENG Ying. Simultaneous determination of five triterpenoid saponins in different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(7): 762-766. doi: 10.3724/SP.J.1123.2014.03009 shu

Simultaneous determination of five triterpenoid saponins in different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry

MA Hui ¹ ,
SONG Shaojiang ² ,
LIU Feifei ² ,
ZHANG Yan ² ,
PENG Ying ^1,,

1.
1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;

Corresponding author: PENG Ying,
Received Date: 10 March 2014
Available Online: 16 April 2014
Fund Project: 国家自然科学青年科学基金项目(81302661). (81302661)

A method was developed for the simultaneous determination of congmunoside Ⅱ, congmunoside Ⅳ, congmunoside Ⅴ, congmunoside Ⅹ and congmuyenoside Ⅱ in different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The chromatographic separation was performed on an Alltima C₁₈ analytical column (250 mm×4.6 mm, 5 μm) using the mobile phases of acetonitrile and 0.1% (v/v) formic acid aqueous solution with gradient elution. The separated compounds were detected in multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI⁺). The linear ranges of congmunoside Ⅱ, congmunoside Ⅳ, congmunoside Ⅴ, congmunoside Ⅹ and congmuyenoside Ⅱ were 0.17-108 μg/L, 0.53-329 μg/L, 0.77-480 μg/L, 0.77-480 μg/L and 0.82-510 μg/L, respectively. The extraction recoveries varied from 99.0% to 100.2%. The developed method is rapid, accurate and reproducible, and is successfully utilized as a quality control method for Aralia elata. The results indicate that the order of contents of these five saponins in different parts of Aralia elata is root bark >leaves >seeds >buds.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- triterpenoid saponins,
- Aralia elata
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