Citation: SUN Ting, WANG Luxiao, ZENG Sanmei, WU Min, YAN Lijuan, ZHANG Feng, HUANG Zhiqiang. Simultaneous determination of twenty psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(7): 702-706. doi: 10.3724/SP.J.1123.2014.03002 shu

Simultaneous determination of twenty psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry

SUN Ting ¹ ,
WANG Luxiao ¹ ,
ZENG Sanmei ¹ ,
WU Min ¹ ,
YAN Lijuan ^1,, ,
ZHANG Feng ² ,
HUANG Zhiqiang ^3,,

1.
1. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China;

Corresponding author: YAN Lijuan, HUANG Zhiqiang,
Received Date: 4 March 2014
Available Online: 15 April 2014
Fund Project: 国家科技支撑计划项目(2009BADB9B02,2012BAK08B01) (2009BADB9B02,2012BAK08B01)国家重大科学仪器设备开发专项项目(2012YQ14000806). (2012YQ14000806)

A method for the simultaneous determination of 20 psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was established. The samples were extracted with alkalified acetonitrile, and then cleaned up using solid phase extraction with an Oasis MCX column. The UPLC separation was performed on a C18 column (50 mm×2.1 mm, 1.7 μm) using a gradient elution with the mobile phases of 0.1% (v/v) formic acid in water and acetonitrile. The ESI-MS/MS detection was achieved in positive mode under multiple reaction monitoring (MRM) mode. The calibration curves showed good linearity in the range of 5-100 μg/L with the correlation coefficients more than 0.99 for the 20 psychoactive drugs. The limits of quantification (LOQ, S/N≥10) for the 20 psychoactive drugs were 5 μg/kg. The average recoveries of the 20 psychoactive drugs spiked in blank pork at three levels of 5, 10 and 50 μg/kg were 66.8%-97.2% with the relative standard deviations from 4.2% to 12.4%. The method is suitable for the simultaneous determination of the 20 psychoactive drugs in pork with the characteristics of easy operation, high accuracy and precision.
1. [1]
  [1] Cooper J, Delahaut P, Fodey T L, et al. Analyst, 2004, 129(2): 169
2. [2]
  [2] Fischer W J, Garthwaite I, Miles C O, et al. Environ Sci Technol, 2001, 35(24): 4849
3. [3]
  [3] Wang Y Y, Jia T. Feed Research (王有月, 贾陶. 饲料研究), 2008(11): 50
4. [4]
  [4] Borges K B, Urias T S, Freire E F, et al. Anal Lett, 2011, 44(18): 2922
5. [5]
  [5] Krogh M, Grefslie H, Rasmussen K E. J Chromatogr B, 1997, 689(2): 357
6. [6]
  [6] Yao L J, Tan J Y, Jiang Z L, et al. Chinese Journal of Analytical Chemistry (姚丽娟, 谭家镒, 姜兆林, 等. 分析化学), 2004, 32(1): 9
7. [7]
  [7] Armagan O. Eur J Med Chem, 2009, 44(12): 4998
8. [8]
  [8] Mercolini L, Mandrioli R, Iannello C, et al. Talanta, 2009, 80(1): 279
9. [9]
  [9] Borrey D, Meyer E, Lambert W, et al. J Chromatogr B, 2001, 765(2): 187
10. [10]
  [10] Wu H Q, Jin Y C, Cai M Z, et al. Chinese Journal of Analytical Chemistry (吴惠勤, 金永春, 蔡明招, 等. 分析化学), 2007, 35(4): 500
11. [11]
  [11] Zhu X L, Liu Q, Li D, et al. Chinese Journal of Veterinary Medicine (朱馨乐, 刘琪, 李丹, 等. 中国兽医杂志), 2009, 43(1): 28
12. [12]
  [12] Irving R C, Dickson S J. Forensic Sci Int, 2007, 166(1): 58
13. [13]
  [13] Lamber K S. Biomed Chromatogr, 2012, 26(1): 1
14. [14]
  [14] Guo D H, Deng X J, Zhao S Z, et al. Chinese Journal of Analytical Chemistry (郭德华, 邓晓军, 赵善贞, 等. 分析化学), 2010, 38(3): 318
15. [15]
  [15] Zhang X Y, Cai X X, Zhang X Y, et al. Chinese Journal of Chromatography (张秀尧, 蔡欣欣, 张晓艺, 等. 色谱), 2010, 28(1): 23
16. [16]
  [16] Xu C B, Suo R, Zhang F, et al. Chinese Journal of Chromatography (许成保, 锁然, 张峰, 等. 色谱), 2012, 30(5): 457
17. [17]
  [17] Kim T, London A, Kharasch E D. J Pharm Biomed Anal, 2011, 55(3): 487
18. [18]
  [18] Pan R N, Hsiong C H, Huang P W, et al. J Anal Toxicol, 2011, 35(3): 170
19. [19]
  [19] Wohlfarth A, Toepfner N, Hermanns-Clausen M, et al. Anal Bioanal Chem, 2011, 400(3): 737
20. [20]
  [20] Kiss B, Bogdan C, Pop A, et al. Talanta, 2012, 99: 649
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