Citation: ZHANG Xiuyao, CAI Xinxin, ZHANG Xiaoyi. Determination of α-solanine,α-chaconine and solanidine in plasma and urine by ultra-performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 586-590. doi: 10.3724/SP.J.1123.2014.03001 shu

Determination of α-solanine,α-chaconine and solanidine in plasma and urine by ultra-performance liquid chromatography-triple quadrupole mass spectrometry

1.
Wenzhou Center for Disease Control and Prevention, Wenzhou 325001, China

Corresponding author: ZHANG Xiuyao,
Received Date: 3 March 2014
Available Online: 26 March 2014
Fund Project: 温州市医学重点学科、温州市第三轮“311”工程建设项目基金(2008012). (2008012)

An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for the determination of α-solanine,α-chaconine and solanidine in plasma and urine. The sample was acidified with aqueous solution containing 2% (v/v if not specified) formic acid, and then cleaned-up by solid-phase extraction with a mixed-mode cation exchange (MCX) cartridge. The analysis of the glycoalkaloids was carried out on an Acquity UPLC BEH C₁₈ column (50 mm×2.1 mm, 1.7 μm) with gradient elution of acetonitrile (containing 0.1% formic acid) and H₂O (containing 0.05% formic acid and 5.0 mmol/L ammonium acetate). The analytes were detected by positive electrospray ionization tandem mass spectrometry in MRM mode, and quantified by external matrix-matched standard calibration. The cycle time of each analysis was 5.5 min. The calibration curves were linear in the range of 0.3-100 ng/mL of the glycoalkaloids in plasma and urine. The correlation coefficients were 0.997-0.999. The limits of detection (S/N=3) and quantitation (S/N=10) were 0.1 ng/mL and 0.3 ng/mL. The average recoveries were 82%-112% and 96%-114% for the glycoalkaloids spiked in plasma and urine, respectively, with relative standard deviations of 4.0%-16% and 2.7%-17% (n=6). The method is simple, accurate and sensitive to detect the glycoalkaloids in plasma and urine for both clinical and forensic purposes.
- ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS),
- tandem mass spectrometry,
- α-solanine,
- α-chaconine,
- solanidine,
- plasma,
- urine,
- potato
1. [1]
  [1] Langkilde S, Mandimika T, Schrder M, et al. Food Chem Toxicol, 2009, 47(6): 1099
2. [2]
  [2] Gong J, Ni S F, Qiu L H, et al. Journal of Anhui Agricultural Sciences (巩江, 倪士峰, 邱莉惠, 等. 安徽农业科学), 2009, 37(9): 4108
3. [3]
  [3] Li M, Xiong X Y, Hu X X, et al. Hunan Agricultural Sciences (李美, 熊兴耀, 胡新喜, 等. 湖南农业科学), 2012(23): 84
4. [4]
  [4] Friedman M. J Agric Food Chem, 2006, 54(23): 8655
5. [5]
  [5] Yang Y, Wang X G, Wang C. Chinese Journal of Public Health (杨煜, 王学工, 王闯. 中国公共卫生), 1995, 11(3): 133
6. [6]
  [6] Xiao W J, Li Q, Xiong X Y, et al. Chinese Journal of Analytical Chemistry (肖文军, 李勤, 熊兴耀, 等. 分析化学), 2011, 39(9): 1459
7. [7]
  [7] Laurila J, Laakso I, Väänänen T, et al. J Agric Food Chem, 1999, 47(7): 2738
8. [8]
  [8] Abell D C, Sporns P. J AOAC Int, 1999, 82(4): 908
9. [9]
  [9] Friedman M, Bautista F F, Stanker L H, et al. J Agric Food Chem, 1998, 46(12): 5097
10. [10]
  [10] Cataldi T R I, Lelario F, Bufo S A. Rapid Commun Mass Spectrom, 2005, 19(21): 3103
11. [11]
  [11] Shakya R, Navarre D A. J Agric Food Chem, 2008, 56(16): 6949
12. [12]
  [12] Matsuda F, Morino K, Miyazawa H, et al. Phytochem Anal, 2004, 15(2): 121
13. [13]
  [13] Sheridan R S, Kemnah J L. J Chromatogr Sci, 2010, 48(10): 790
14. [14]
  [14] Wu H M, Zeng J, Li M, et al. Food Science (伍慧敏, 曾静, 李美, 等. 食品科学), 2013, 34(24): 121
15. [15]
  [15] Hellenas K E, Nyman A, Slanina P, et al. J Chromatogr Biomed Appl, 1992, 573(1): 69
16. [16]
  [16] Chen C, Yan H, Shen B H, et al. Chinese Journal of Chromatography (陈聪, 严慧, 沈保华, 等. 色谱), 2012, 30(5): 445
17. [17]
  [17] Tang Y, Zhang S Q, Li X, et al. Chinese Journal of Chromatography (汤瑶, 张双庆, 李响, 等. 色谱), 2012, 30(2): 154
18. [18]
  [18] Zhang X Y, Cai X X, Zhang X Y. Chinese Journal of Chromatography (张秀尧, 蔡欣欣, 张晓艺. 色谱), 2010, 28(1): 23
19. [19]
  [19] Commission Decision 2002/657/EC of 12 August 2002. Implementing Council Directive 96/23/EC Concerning the Performance of Analytical Methods and the Interpretation of Results (2002). Off J Eur Comm, L221: 8
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