Citation: ZHANG Lu, KONG Xianghong, HE Qiang, ZHANG Longzhuang, LI Jianhua. Determination of five imidazole pesticide residues in fruits by Turbo flow online purification-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 559-565. doi: 10.3724/SP.J.1123.2014.02001 shu

Determination of five imidazole pesticide residues in fruits by Turbo flow online purification-ultra performance liquid chromatography-tandem mass spectrometry

1.
1. Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi'an 710068, China;

Corresponding author: ZHANG Lu,
Received Date: 17 February 2014
Available Online: 19 March 2014
Fund Project: 陕西出入境检验检疫局项目(陕K-201203). (陕K-201203)

A Turbo flow-ultra performance liquid chromatography-tandem mass spectrometry (TF-UPLC-MS/MS) method was developed for the determination of five imidazole pesticides (imazapyr, triazoxide, rabenzazole, prochloraz and fenamidone) in fruits. The fruit samples were dissolved in saturated sodium chloride solution and extracted by acetonitrile. After the acetonitrile layer was evaporated and redissolved with acetonitrile-water (1:1, v/v), the fruit samples were analyzed by TF-UPLC-MS/MS. The main factors influencing the purification efficiency including the TF column, mobile phase, elution solution and elution rate were optimized. Under the optimized experimental conditions, the analytes were purified by Turbo flow C18 column (50 mm×1.0 mm) and separated on a Hypersil GOLD aQ column (100 mm×2.1 mm) using the mobile phases of acetonitrile and 5 mmol/L ammonium acetate (containing 0.1% (v/v) formic acid) aqueous solution with gradient elution. The compounds were detected by selective reaction monitoring (SRM) via positive electrospray ionization (ESI⁺). The linear range of the method ranged from 0.0075 to 0.75 mg/L for all the five imidazole pesticides, with the correlation coefficients (r²) greater than 0.99. The limits of quantification were 0.005 mg/kg for all the five imidazole pesticides. The recoveries were in the range from 71.2% to 122.4% at the spiked levels of 0.005, 0.01, 0.05 and 0.5 mg/kg with the relative standard deviations ranging from 0.5% to 8.9% in actual samples. The results indicate that the developed method is simple, efficient and precise, and can be a reliable technique for the determination of the five imidazole pesticides in fruit samples.
1. [1]
  [1] Li F, Hu J M, Li F G, et al. Chinese Journal of Health Laboratory Technology (李芳, 胡建民, 李锋格, 等. 中国卫生检验杂志), 2011, 2 (10): 2416
2. [2]
  [2] Liu X C, Chen P, Shen J, et al. Journal of Analytical Science (柳训才, 陈平, 沈菁, 等. 分析科学学报), 2007, 23 (5): 523
3. [3]
  [3] Li C, Suo R, Wang F C, et al. Chinese Journal of Chromatography (李成, 锁然, 王凤池, 等. 色谱), 2008, 26 (6): 726
4. [4]
  [4] Zhao Z Y, Shi Z H, Kang J, et al. Chinese Journal of Chromatography (赵志远, 石志红, 康健, 等. 色谱), 2013, 31 (4): 372
5. [5]
  [5] Chen X L, Li Z X, Cao Z Y, et al. Chinese Journal of Chromatography (陈小龙, 李正翔, 曹赵云, 等. 色谱), 2013, 31 (10): 954
6. [6]
  [6] Huang J R, Kong X H, Yao B H, et al. Chinese Journal of Chromatography (黄江锐, 孔祥虹, 姚秉华, 等. 色谱), 2011, 29(10): 974
7. [7]
  [7] Mou Y L, Guo D H, Ding Z P. Chinese Journal of Chromatography (牟艳莉, 郭德华, 丁卓平. 色谱), 2013, 31(10): 1016
8. [8]
  [8] Xu X L, Zhao H X, Li L, et al. Chinese Journal of Chromatography (许秀丽, 赵海香, 李礼, 等. 色谱), 2012, 30(3): 267
9. [9]
  [9] Ouyang Y F, Tang H B, Wu Y, et al. Chinese Journal of Chromatography (欧阳运富, 唐宏兵, 吴英, 等. 色谱), 2012, 30(7): 654
10. [10]
  [10] Ma Z L, Zhao W, Li L Y, et al. Chinese Journal of Chromatography (马智玲, 赵文, 李凌云, 等. 色谱), 2013, 31(3): 228
11. [11]
  [11] Lin H D, Zou Z F, Qin Y, et al. Chinese Journal of Chromatography (林海丹, 邹志飞, 秦燕, 等. 色谱), 2013, 31(2): 170
12. [12]
  [12] Song Y.[MS Dissertation]. Beijing: Chinese Academy of Agriculture Sciences (宋莹.[硕士学位论文]. 北京: 中国农业科学院), 2011
13. [13]
  [13] Lehotay S J, de Kok A, Hiemstra M, et al. J AOAC Int, 2005, 88(2): 595
14. [14]
  [14] Paya P, Anastassiades M, Mack D, et al. Anal Bioanal Chem, 2007, 389(6): 1697
15. [15]
  [15] Shi Y, Lafontaine C, Berube M, et al. Thermo Scientific Application Note 568.[2014-02-17]. http://www.cohesivetech.Com/publications/search-publications/articles_detail.cfm?file_id=239&mode=list&author=&keywords=
16. [16]
  [16] Garcia-Galan M J, Diaz-Cruz M S, Barcelo D. Talanta, 2010, 81: 355
17. [17]
  [17] Fayad P B, Prévost M, Sauvé S. Talanta, 2013, 115: 349
18. [18]
  [18] Liu R Z, Ruan T, Wang T, et al. Talanta, 2014, 120: 158
1. [1]
  Yahui Ma , Leiyang Lv . Pears on the Journey of Fruits. University Chemistry, 2024, 39(9): 64-73. doi: 10.12461/PKU.DXHX202404029
2. [2]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
3. [3]
  Wuxin Bai , Qianqian Zhou , Zhenjie Lu , Ye Song , Yongsheng Fu . Co-Ni Bimetallic Zeolitic Imidazolate Frameworks Supported on Carbon Cloth as Free-Standing Electrode for Highly Efficient Oxygen Evolution. Acta Physico-Chimica Sinica, 2024, 40(3): 2305041-0. doi: 10.3866/PKU.WHXB202305041
4. [4]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
5. [5]
  Aoyun Meng , Zhenhua Li , Guoyuan Xiong , Zhen Li , Jinfeng Zhang . S-scheme heterojunction Al₆Si₂O₁₃/BiOBr with enhanced charge transfer effect for efficient and stable photocatalytic degradation of triazophos and dichlorvos pesticides. Acta Physico-Chimica Sinica, 2026, 42(5): 100186-0. doi: 10.1016/j.actphy.2025.100186
6. [6]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
7. [7]
  Jingzhao Cheng , Shiyu Gao , Bei Cheng , Kai Yang , Wang Wang , Shaowen Cao . Construction of 4-Amino-1H-imidazole-5-carbonitrile Modified Carbon Nitride-Based Donor-Acceptor Photocatalyst for Efficient Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2024, 40(11): 2406026-0. doi: 10.3866/PKU.WHXB202406026
8. [8]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
9. [9]
  Yuxia Gao , Li Zhang , Chenhui Zhang , Fengpei Du . Chemical Empowerment for Green Development of Pesticides: From Molecular Design to Field Application. University Chemistry, 2025, 40(12): 78-86. doi: 10.12461/PKU.DXHX202509052
10. [10]
  Chen LU , Qinlong HONG , Haixia ZHANG , Jian ZHANG . Syntheses, structures, and properties of copper-iodine cluster-based boron imidazolate framework materials. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 149-154. doi: 10.11862/CJIC.20240407
11. [11]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064
12. [12]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
13. [13]
  Guang Huang , Lei Li , Dingyi Zhang , Xingze Wang , Yugai Huang , Wenhui Liang , Zhifen Guo , Wenmei Jiao . Cobalt’s Valor, Nickel’s Foe: A Comprehensive Chemical Experiment Utilizing a Cobalt-based Imidazolate Framework for Nickel Ion Removal. University Chemistry, 2024, 39(8): 174-183. doi: 10.3866/PKU.DXHX202311051
14. [14]
  Wenjie SHI , Fan LU , Mengwei CHEN , Jin WANG , Yingfeng HAN . Synthesis and host-guest properties of imidazolium-functionalized zirconium metal-organic cage. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 105-113. doi: 10.11862/CJIC.20240360
15. [15]
  Qiang Zhang , Yuanbiao Huang , Rong Cao . Imidazolium-Based Materials for CO₂ Electroreduction. Acta Physico-Chimica Sinica, 2024, 40(4): 2306040-0. doi: 10.3866/PKU.WHXB202306040
16. [16]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
17. [17]
  Xiaohong Li , Huanjiang Wang , Fang Tan , Xiaohua Cai , Yingchun Luo , Yanli Leng , Guoyong Zhou , Zhu Wen . Research on the Project-Driven Teaching Model in the Context of New Engineering: A Case Study of the Development of a “Portable Metal ions and Pesticide Residues Intelligent Detection System”. University Chemistry, 2026, 41(1): 204-212. doi: 10.12461/PKU.DXHX202510037
18. [18]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
19. [19]
  Bing Yuan , Fengli Yu , Congxia Xie . Teaching Cases Design of Catalysis Courses for Emerging Engineering Education. University Chemistry, 2024, 39(3): 191-198. doi: 10.3866/PKU.DXHX202309032
20. [20]
  Qian Shao , Jiajing Tan , Yongmei Chen , Jiyue Jing , Zhuo Wang . Exploration and Practice on the Management of Extracurricular Innovation Laboratories in Chemistry. University Chemistry, 2024, 39(4): 19-25. doi: 10.3866/PKU.DXHX202310119

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(534)
HTML views(17)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142