Citation: ZHANG Lu, KONG Xianghong, HE Qiang, ZHANG Longzhuang, LI Jianhua. Determination of five imidazole pesticide residues in fruits by Turbo flow online purification-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 559-565. doi: 10.3724/SP.J.1123.2014.02001 shu

Determination of five imidazole pesticide residues in fruits by Turbo flow online purification-ultra performance liquid chromatography-tandem mass spectrometry

1.
1. Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi'an 710068, China;

Corresponding author: ZHANG Lu,
Received Date: 17 February 2014
Available Online: 19 March 2014
Fund Project: 陕西出入境检验检疫局项目(陕K-201203). (陕K-201203)

A Turbo flow-ultra performance liquid chromatography-tandem mass spectrometry (TF-UPLC-MS/MS) method was developed for the determination of five imidazole pesticides (imazapyr, triazoxide, rabenzazole, prochloraz and fenamidone) in fruits. The fruit samples were dissolved in saturated sodium chloride solution and extracted by acetonitrile. After the acetonitrile layer was evaporated and redissolved with acetonitrile-water (1:1, v/v), the fruit samples were analyzed by TF-UPLC-MS/MS. The main factors influencing the purification efficiency including the TF column, mobile phase, elution solution and elution rate were optimized. Under the optimized experimental conditions, the analytes were purified by Turbo flow C18 column (50 mm×1.0 mm) and separated on a Hypersil GOLD aQ column (100 mm×2.1 mm) using the mobile phases of acetonitrile and 5 mmol/L ammonium acetate (containing 0.1% (v/v) formic acid) aqueous solution with gradient elution. The compounds were detected by selective reaction monitoring (SRM) via positive electrospray ionization (ESI⁺). The linear range of the method ranged from 0.0075 to 0.75 mg/L for all the five imidazole pesticides, with the correlation coefficients (r²) greater than 0.99. The limits of quantification were 0.005 mg/kg for all the five imidazole pesticides. The recoveries were in the range from 71.2% to 122.4% at the spiked levels of 0.005, 0.01, 0.05 and 0.5 mg/kg with the relative standard deviations ranging from 0.5% to 8.9% in actual samples. The results indicate that the developed method is simple, efficient and precise, and can be a reliable technique for the determination of the five imidazole pesticides in fruit samples.
1. [1]
  [1] Li F, Hu J M, Li F G, et al. Chinese Journal of Health Laboratory Technology (李芳, 胡建民, 李锋格, 等. 中国卫生检验杂志), 2011, 2 (10): 2416
2. [2]
  [2] Liu X C, Chen P, Shen J, et al. Journal of Analytical Science (柳训才, 陈平, 沈菁, 等. 分析科学学报), 2007, 23 (5): 523
3. [3]
  [3] Li C, Suo R, Wang F C, et al. Chinese Journal of Chromatography (李成, 锁然, 王凤池, 等. 色谱), 2008, 26 (6): 726
4. [4]
  [4] Zhao Z Y, Shi Z H, Kang J, et al. Chinese Journal of Chromatography (赵志远, 石志红, 康健, 等. 色谱), 2013, 31 (4): 372
5. [5]
  [5] Chen X L, Li Z X, Cao Z Y, et al. Chinese Journal of Chromatography (陈小龙, 李正翔, 曹赵云, 等. 色谱), 2013, 31 (10): 954
6. [6]
  [6] Huang J R, Kong X H, Yao B H, et al. Chinese Journal of Chromatography (黄江锐, 孔祥虹, 姚秉华, 等. 色谱), 2011, 29(10): 974
7. [7]
  [7] Mou Y L, Guo D H, Ding Z P. Chinese Journal of Chromatography (牟艳莉, 郭德华, 丁卓平. 色谱), 2013, 31(10): 1016
8. [8]
  [8] Xu X L, Zhao H X, Li L, et al. Chinese Journal of Chromatography (许秀丽, 赵海香, 李礼, 等. 色谱), 2012, 30(3): 267
9. [9]
  [9] Ouyang Y F, Tang H B, Wu Y, et al. Chinese Journal of Chromatography (欧阳运富, 唐宏兵, 吴英, 等. 色谱), 2012, 30(7): 654
10. [10]
  [10] Ma Z L, Zhao W, Li L Y, et al. Chinese Journal of Chromatography (马智玲, 赵文, 李凌云, 等. 色谱), 2013, 31(3): 228
11. [11]
  [11] Lin H D, Zou Z F, Qin Y, et al. Chinese Journal of Chromatography (林海丹, 邹志飞, 秦燕, 等. 色谱), 2013, 31(2): 170
12. [12]
  [12] Song Y.[MS Dissertation]. Beijing: Chinese Academy of Agriculture Sciences (宋莹.[硕士学位论文]. 北京: 中国农业科学院), 2011
13. [13]
  [13] Lehotay S J, de Kok A, Hiemstra M, et al. J AOAC Int, 2005, 88(2): 595
14. [14]
  [14] Paya P, Anastassiades M, Mack D, et al. Anal Bioanal Chem, 2007, 389(6): 1697
15. [15]
  [15] Shi Y, Lafontaine C, Berube M, et al. Thermo Scientific Application Note 568.[2014-02-17]. http://www.cohesivetech.Com/publications/search-publications/articles_detail.cfm?file_id=239&mode=list&author=&keywords=
16. [16]
  [16] Garcia-Galan M J, Diaz-Cruz M S, Barcelo D. Talanta, 2010, 81: 355
17. [17]
  [17] Fayad P B, Prévost M, Sauvé S. Talanta, 2013, 115: 349
18. [18]
  [18] Liu R Z, Ruan T, Wang T, et al. Talanta, 2014, 120: 158
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沈阳化工大学材料科学与工程学院沈阳 110142