Citation: XIA Guanghui, SHEN Weijian, YU Keyao, WU Bin, ZHANG Rui, SHEN Chongyu, ZHAO Zengyun, BIAN Xiaohong, XU Jiyang. Determination of four insecticide residues in honey and royal jelly by gas chromatography-negative chemical ionization mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(7): 741-745. doi: 10.3724/SP.J.1123.2014.01043 shu

Determination of four insecticide residues in honey and royal jelly by gas chromatography-negative chemical ionization mass spectrometry

1.
1. Department of Biochemistry, China Pharmaceutical University, Nanjing 210009, China;

Corresponding author: XU Jiyang,
Received Date: 26 January 2014
Available Online: 5 May 2014
Fund Project: 国家质检总局科技计划项目(2011KJ39,2012KJ31,2012IK167). (2011KJ39,2012KJ31,2012IK167)

A method was developed for the determination of four insecticide residues in honey and royal jelly by gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS). The honey and royal jelly samples were treated with different preparation methods as the result of the different components. The honey sample was extracted with ethyl acetate and cleaned up with primary second amine, and the royal jelly sample was extracted with acetonitrile-water (1:1, v/v), and cleaned up with a C18 solid-phase extraction column. Finally, the extracts of the honey and royal jelly were analyzed by GC-NCI/MS in selected ion monitoring (SIM) mode separately. External standard calibration method was used for quantification. The linearities of calibration curves of the four insecticides were good with the correlation coefficients greater than 0.99 in the range of 50-500 μg/L. The limits of the detection (LODs) of the four insecticides were in the range of 0.12-5.0 μg/kg, and the limits of the quantification (LOQs) were in the range of 0.40-16.5 μg/kg. The recoveries of the four insecticides spiked in honey and royal jelly at three spiked levels (10, 15 and 20 μg/kg) were in the range of 78.2%-110.0%, and the relative standard deviations (RSDs) were all below 14%. The sensitivity and selectivity of this method were good with no interfering peaks. The proposed method is simple, quick and effective to analyze the four insecticide residues in honey and royal jelly.
- gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS),
- insecticides,
- honey,
- royal jelly
1. [1]
  [1] Majtan J, Kumar P, Majtan T, et al. Exp Dermatol, 2010, 19(8): 73
2. [2]
  [2] Mukherjee I. Bull Environ Contam Toxicol, 2009, 83(6): 818
3. [3]
  [3] Girisgin A, Aydin L. Uludag Bee J, 2010, 10(2): 70
4. [4]
  [4] Choudhary A, Sharma D, Badiyala A. Pestic Res J, 2009, 21(1): 67
5. [5]
  [5] Karazafiris E, Tananaki C, Menkissoglu-Spiroudi U, et al. Pestic Manag Sci, 2008, 64(2): 165
6. [6]
  [6] Johnson R M, Ellis M D, Mullin C A, et al. Apidologie, 2010, 41(3): 312
7. [7]
  [7] Smodis Skerl M I, Nakrst M, Zvokelj L, et al. Acta Vet Brno, 2011, 80(1): 51
8. [8]
  [8] Sharma D, Nagpal A, Pakade Y B, et al. Talanta, 2010, 82(4): 1077
9. [9]
  [9] Jiménez J, Bernal J, del Nozal M J, et al. J Chromatogr A, 2001, 919(1): 147
10. [10]
  [10] Li X B, Zhao M, Li S Q, et al. Chinese Journal of Chromatography (李贤波, 赵蔓, 李胜清, 等. 色谱), 2012, 30(9): 926
11. [11]
  [11] Shinger M I, Elbashir A A, Ahmed H E, et al. Biomed Chromatogr, 2012, 26(5): 589
12. [12]
  [12] Shen W J, Cao X W, Liu Y J, et al. Chinese Journal of Chromatography (沈伟健, 曹孝文, 刘一军, 等. 色谱), 2012, 30(11): 1172
13. [13]
  [13] Li H Y, Zhang Q, Kang S Y, et al. Chinese Journal of Chromatography (李海玉, 张庆, 康苏媛, 等. 色谱), 2012, 30(6): 596
14. [14]
  [14] Nguyen T D, Lee M H, Lee G H. Microchem J, 2010, 95(1): 113
15. [15]
  [15] Shen C Y, Cao X W, Shen W J, et al. Talanta, 2011, 84(1): 141
16. [16]
  [16] Martel A C, Zeggane S. J Chromatogr A, 2002, 954(1): 173
17. [17]
  [17] Chung S W, Lam C. Anal Bioanal Chem, 2012, 403(3): 885
18. [18]
  [18] Ball D W. J Chem Educ, 2007, 84(10): 1643
19. [19]
  [19] Ramanauskiene K, Stelmakiene A, Briedis V, et al. Food Chem, 2012, 132(3): 1544
20. [20]
  [20] Sabatini A G, Marcazzan G L, Caboni M F, et al. J ApiProd ApiMed Sci, 2009, 1(1): 1
21. [21]
  [21] Daniele G, Casabianca H. Food Chem, 2012, 134(2): 1025
1. [1]
  Junyuan Zhang , Zhiwei Miao . 有机磷杀虫剂的前世今生. University Chemistry, 2025, 40(6): 129-138. doi: 10.12461/PKU.DXHX202408118
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
4. [4]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
5. [5]
  Yuanchun Pan , Xinyun Lin , Leyi Yang , Wenya Hu , Dekui Song , Nan Liu . Artificial Intelligence Science Practice: Preparation of Electronic Skin by Chemical Vapor Deposition of Graphene. University Chemistry, 2025, 40(11): 272-280. doi: 10.12461/PKU.DXHX202412052
6. [6]
  Ruilan Fan , Xiaoling Huang . 磷源的选择及三种含磷阻燃剂的合成与阻燃性. University Chemistry, 2025, 40(8): 181-191. doi: 10.12461/PKU.DXHX202410025
7. [7]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
8. [8]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
9. [9]
  Xichen YAO , Shuxian WANG , Yun WANG , Cheng WANG , Chuang ZHANG . Oxygen reduction performance of self?supported Fe/N/C three-dimensional aerogel catalyst layers. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1387-1396. doi: 10.11862/CJIC.20240384
10. [10]
  Hailang JIA , Yujie LU , Pengcheng JI . Preparation and properties of nitrogen and phosphorus co-doped graphene carbon aerogel supported ruthenium electrocatalyst for hydrogen evolution reaction. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2327-2336. doi: 10.11862/CJIC.20250021
11. [11]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
12. [12]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
13. [13]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
14. [14]
  Yuanyuan JIANG , Fangfang TU , Yuhong ZHANG , Shi CHEN , Jiayuan XIANG , Xinhui XIA . Preparation and electrochemical properties of high-stability cathode prelithiation additive. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1101-1111. doi: 10.11862/CJIC.20240441
15. [15]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
16. [16]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
17. [17]
  Congying Lu , Fei Zhong , Zhenyu Yuan , Shuaibing Li , Jiayao Li , Jiewen Liu , Xianyang Hu , Liqun Sun , Rui Li , Meijuan Hu . Experimental Improvement of Surfactant Interface Chemistry: An Integrated Design for the Fusion of Experiment and Simulation. University Chemistry, 2024, 39(3): 283-293. doi: 10.3866/PKU.DXHX202308097
18. [18]
  Lutian Zhao , Yangge Guo , Liuxuan Luo , Xiaohui Yan , Shuiyun Shen , Junliang Zhang . Electrochemical Synthesis for Metallic Nanocrystal Electrocatalysts: Principle, Application and Challenge. Acta Physico-Chimica Sinica, 2024, 40(7): 2306029-0. doi: 10.3866/PKU.WHXB202306029
19. [19]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
20. [20]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(819)
HTML views(83)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142