Citation: WANG Yun, HUANG Zhiqiang, YE Ying, ZHANG Ying, XIAO Shuiyuan. Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 658-661. doi: 10.3724/SP.J.1123.2014.01023 shu

Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry

WANG Yun ^1,4 ,
HUANG Zhiqiang ^2,, ,
YE Ying ³ ,
ZHANG Ying ² ,
XIAO Shuiyuan ^1,,

1.
1. School of Public Health, Central South University, Changsha 410078, China;

Corresponding author: HUANG Zhiqiang, XIAO Shuiyuan,
Received Date: 13 January 2014
Available Online: 30 March 2014
Fund Project: 国家自然科学基金项目(81273089) (81273089)中南大学博士生创新基金项目(2013zzts076). (2013zzts076)

An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The samples were extracted and purified with chloroform. The extracts were determined by GC-MS/MS in multiple reaction monitoring (MRM) mode. The cotinine-d₃ as an isotope internal standard was applied to quantify and confirm the urinary cotinine of children exposed to passive smoking. The method had a good linearity from 0.1 μg/L to 10 μg/L with the correlation coefficient (r)>0.998. The recoveries of the cotinine in blank urine were from 79.2% to 112.8% at spiked levels of 0.1, 1.0 and 10 μg/L, with relative standard deviations (RSDs) from 2.1% to 5.8%. The limit of quantification (LOQ) of the method was 0.1 μg/L. The developed method is accurate, sensitive, rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.
- gas chromatography-tandem mass spectrometry (GC-MS/MS),
- isotope dilution,
- cotinine,
- urine,
- children,
- passive smoking
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