Citation: WANG Jingwen, HUANG Xianglu, CAO Jin, WANG Gangli, ZHANG Qingsheng, DING Lixia. Simultaneous determination of 25 illegally added drugs in diet health foods by ultra-high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(2): 151-156. doi: 10.3724/SP.J.1123.2013.10015 shu

Simultaneous determination of 25 illegally added drugs in diet health foods by ultra-high performance liquid chromatography

1.
1. National Institutes for Food and Drug Control, Beijing 100050, China;

Corresponding author: CAO Jin, WANG Gangli,
Received Date: 16 October 2013
Available Online: 30 November 2013
Fund Project: 国家“十二五”科技支撑计划项目(2011BAZ02015-2). (2011BAZ02015-2)

An analytical method using ultra-high performance liquid chromatography (UPLC) was developed for qualitative and quantitative analysis of 25 illegally added drugs in diet health foods. The diet food samples were extracted using 40 mL methanol by sonication. After centrifugation, the supernatants were separated on a Waters HSS T3 column with gradient elution at a flow rate of 0.3 mL/min, coupling with diode array detection (DAD) in wavelength range from 200 nm to 400 nm. The binary mobile phase was acetonitrile and 10 mmol/L ammonium acetate solution (containing 0.1% formic acid). The correlation coefficient of standard curve for each drug in linearity range was not less than 0.997, as well as the recoveries of all the drugs in diet health foods were 70.7%-104% with the relative standard deviations (RSDs) of 0.132%-5.03% at three spiked levels. Seventeen diet food samples were tested, in which phenolphthalein was found in three samples and emodin was found in one sample. The method is specific, easy, quick, and suitable for confirmation of the 25 illegally added drugs in diet health foods.
- ultra-high performance liquid chromatography (UPLC),
- illegally added drugs,
- diet health foods
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沈阳化工大学材料科学与工程学院沈阳 110142