Citation: CHEN Lei, LUAN Jun, FEI Xiaoqing, WU Bin, SHEN Chongyu, ZHANG Rui. Determination of methylglyoxal in Manuka honey of New Zealand by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(2): 189-193. doi: 10.3724/SP.J.1123.2013.10011 shu

Determination of methylglyoxal in Manuka honey of New Zealand by high performance liquid chromatography

1.
Animal, Plant and Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China

Corresponding author: CHEN Lei,
Received Date: 12 October 2013
Available Online: 9 December 2013
Fund Project: 国家质检总局科技计划项目(2013KJ40) (2013KJ40)国家质检总局科技计划项目(2011IK209). (2011IK209)

An HPLC method was developed for the determination of methylglyoxal in Manuka honey of New Zealand. The honey sample was dissolved in water and mixed with o-phenylenediamine solution for derivatization. After the reaction for at least 8 h in the dark at room temperature, the solution was filtered with 0.22 μm membrane and injected into an HPLC system for analysis. The separation was carried out on a Kromasil reversed phase column with gradient elution. The mobile phases were methanol and 0.1% (v/v) acetic acid aqueous solution. The detection wavelength was 318 nm. The external standard method was used for quantitation. The linear range of methylglyoxal was 1-50 mg/L with a correlation coefficient of 0.9999. The LOD (S/N=3) and LOQ (S/N=10) were 0.02 mg/L and 0.06 mg/L, respectively. The recoveries at the spiked levels of 50, 100, 200 mg/kg were 98.3%-101.5% and the RSDs (n=5) were less than 5%. The derivative of methylglyoxal was stable within 24 h. The results showed that the pretreatment of this method is simple and the sensitivity, the recovery and repeatability are good. This method can be used for the quality control of Manuka honey of New Zealand, and also for the detection of methylglyoxal in Chinese honey.
- high performance liquid chromatography (HPLC),
- methylglyoxal (MGO),
- Manuka,
- honey
1. [1]
  [1] Zhu W, Hu F L, Li Y H, et al. Natural Product Research and Development (朱威, 胡福良, 李英华, 等. 天然产物研究与开发), 2004, 16(4): 372
2. [2]
  [2] Xuan H Z, Hu F L. Journal of Bee (玄红专, 胡福良. 蜜蜂杂志), 2002(9): 23
3. [3]
  [3] Allen K L, Molan P C, Reid G M. J Pharm Pharmacol, 1991, 43(12): 817
4. [4]
  [4] Mavric E, Wittmann S, Barth G, et al. Mol Nutr Food Res, 2008, 52(4): 483
5. [5]
  [5] Adams C J, Boult C H, Deadman B J, et al. Carbohydr Res, 2008, 343(4): 651
6. [6]
  [6] Sun Y P, Li P. Occupation and Health (孙艳萍, 李萍. 职业与健康), 2012, 28(19): 2366
7. [7]
  [7] Lü C, Ding T, Ma X, et al. Chinese Journal of Chromatography (吕辰, 丁涛, 马昕, 等. 色谱), 2013, 31(11): 1046
8. [8]
  [8] Li Y, Shao M, Lu S H. Environmental Chemistry (李阳, 邵敏, 陆思华. 环境化学), 2009, 28(5): 630
9. [9]
  [9] Mu C C, Feng Y L, Zhai J Q, et al. Chinese Journal of Analytical Chemistry (牟翠翠, 冯艳丽, 翟金清, 等. 分析化学), 2010, 38(11): 1573
10. [10]
  [10] Feng Y L, Mu C C, Fu Z R, et al. Chinese Journal of Analytical Chemistry (冯艳丽, 牟翠翠, 付正茹, 等. 分析化学), 2011, 39(11): 1653
11. [11]
  [11] Zou T, Feng Y L, Fu Z R, et al. Acta Scientiae Circumstantiae (邹婷, 冯艳丽, 付正茹, 等. 环境科学学报), 2012, 32(11): 2718
12. [12]
  [12] Weigel K, Opitz T, Henle T. Eur Food Res Technol, 2004, 218(2): 147
13. [13]
  [13] Oelschlaegel S, Gruner M, Wang P N, et al. J Agric Food Chem, 2012, 60(29): 7229
14. [14]
  [14] Stephens J M, Schlothauer R C, Morris B D, et al. Food Chem, 2010, 120(1): 78
15. [15]
  [15] Arena E, Ballistreri G, Tomaselli F, et al. J Food Sci, 2011, 76(8): C1203
1. [1]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
2. [2]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
3. [3]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
4. [4]
  Jie Li , Huida Qian , Deyang Pan , Wenjing Wang , Daliang Zhu , Zhongxue Fang . Efficient Synthesis of Anethaldehyde Induced by Visible Light. University Chemistry, 2024, 39(4): 343-350. doi: 10.3866/PKU.DXHX202310076
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
7. [7]
  Yanglin Jiang , Mingqing Chen , Min Liang , Yige Yao , Yan Zhang , Peng Wang , Jianping Zhang . Experimental and Theoretical Investigations of Solvent Polarity Effect on ESIPT Mechanism in 4′-N,N-diethylamino-3-hydroxybenzoflavone. Acta Physico-Chimica Sinica, 2025, 41(2): 100012-. doi: 10.3866/PKU.WHXB202309027
8. [8]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
9. [9]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
10. [10]
  Yikai Wang , Xiaolin Jiang , Haoming Song , Nan Wei , Yifan Wang , Xinjun Xu , Cuihong Li , Hao Lu , Yahui Liu , Zhishan Bo . 氰基修饰的苝二酰亚胺衍生物作为膜厚不敏感型阴极界面材料用于高效有机太阳能电池. Acta Physico-Chimica Sinica, 2025, 41(3): 2406007-. doi: 10.3866/PKU.WHXB202406007
11. [11]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
12. [12]
  Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351
13. [13]
  Xiaoning TANG , Junnan LIU , Xingfu YANG , Jie LEI , Qiuyang LUO , Shu XIA , An XUE . Effect of sodium alginate-sodium carboxymethylcellulose gel layer on the stability of Zn anodes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1452-1460. doi: 10.11862/CJIC.20240191
14. [14]
  Yue Zhao , Yanfei Li , Tao Xiong . Copper Hydride-Catalyzed Nucleophilic Additions of Unsaturated Hydrocarbons to Aldehydes and Ketones. University Chemistry, 2024, 39(4): 280-285. doi: 10.3866/PKU.DXHX202309001
15. [15]
  Chi Li , Jichao Wan , Qiyu Long , Hui Lv , Ying Xiong . N-Heterocyclic Carbene (NHC)-Catalyzed Amidation of Aldehydes with Nitroso Compounds. University Chemistry, 2024, 39(5): 388-395. doi: 10.3866/PKU.DXHX202312016
16. [16]
  Ling Liu , Haibin Wang , Genrong Qiang . Curriculum Ideological and Political Design for the Comprehensive Preparation Experiment of Ethyl Benzoate Synthesized from Benzyl Alcohol. University Chemistry, 2024, 39(2): 94-98. doi: 10.3866/PKU.DXHX202304080
17. [17]
  Shuying Zhu , Shuting Wu , Ou Zheng . Improvement and Expansion of the Experiment for Determining the Rate Constant of the Saponification Reaction of Ethyl Acetate. University Chemistry, 2024, 39(4): 107-113. doi: 10.3866/PKU.DXHX202310117
18. [18]
  Ruitong Zhang , Zhiqiang Zeng , Xiaoguang Zhang . Improvement of Ethyl Acetate Saponification Reaction and Iodine Clock Reaction Experiments. University Chemistry, 2024, 39(8): 197-203. doi: 10.3866/PKU.DXHX202312004
19. [19]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
20. [20]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(713)
HTML views(97)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142