Citation: XIONG Zhiyu, DONG Ying, ZHOU Hongbin, YU Yang, LI Jing, SUN Li. Simultaneous determination of 16 organic acids in feed additives by on-line enrichment and ion chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(2): 145-150. doi: 10.3724/SP.J.1123.2013.10003 shu

Simultaneous determination of 16 organic acids in feed additives by on-line enrichment and ion chromatography-mass spectrometry

XIONG Zhiyu ¹ ,
DONG Ying ^1,, ,
ZHOU Hongbin ^2,, ,
YU Yang ² ,
LI Jing ² ,
SUN Li ²

1.
1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;

Corresponding author: DONG Ying, ZHOU Hongbin,
Received Date: 9 October 2013
Available Online: 3 December 2013
Fund Project: 江苏出入境检验检疫局科研项目(2010KJ41). (2010KJ41)

A novel analytical method for simultaneous determination of sixteen organic acids by on-line enrichment and ion chromatography-mass spectrometry (IC-MS) was developed. On-line enrichment and separation of the organic acids were performed by ion chromatography on a homemade enrichment column and a homemade separation column. The qualitative and quantitative analyses of the organic acids were performed by mass spectrometry in selected ion monitoring (SIM) mode on the basis of atmospheric pressure chemical ionization (APCI) source in negative mode. The sample of 200 μL was injected for the analysis, and the on-line enrichment time was 3 min. The sodium hydroxide solution was used as a gradient elution system. The two columns made it possible to have a low limit of detection due to the good enrichment and separation capability. The sixteen organic acids were separated completely within 30 min. All curves showed good linearity within the test concentration ranges. The limits of detection (LODs) were between 0.01 and 0.22 mg/L, and the average recoveries were between 70.6% and 110.8%. The relative standard deviations (RSDs) were less than 6.3%. The results indicate that this method is simple, rapid, sensitive and accurate for the determination of the organic acids in feed additives.
- on-line enrichment,
- ion chromatography-mass spectrometry (IC-MS),
- organic acids,
- feed additives
1. [1]
  [1] Chen C Y, Wang T. Feed Research (陈才勇, 王恬. 饲料研究), 2004(1): 37
2. [2]
  [2] Chen Y, Zhen L. China Feed (陈勇, 甄莉. 中国饲料), 2004(9): 30
3. [3]
  [3] Ma J F, Ma Y L. Feed Industry (马吉锋, 马玉龙. 饲料工业), 2005, 26(10): 17
4. [4]
  [4] Li C L, Zhou A G, Wang Z S. Feed Industry (李成良, 周安国, 王之盛. 饲料工业), 2006, 27(11): 35
5. [5]
  [5] Liu X T, Zhang W M, Jiang H F, et al. Food Research and Development (刘晓棠, 张卫明, 姜洪芳, 等. 食品研究与开发), 2010, 31(1): 100
6. [6]
  [6] Niu J G, Liang X J, Liu X, et al. Chinese Journal of Applied Chemistry (牛金刚, 梁晓静, 刘霞, 等. 应用化学), 2010, 27(3): 342
7. [7]
  [7] Tang Y J, Wu M J. Food Chem, 2007, 103: 243
8. [8]
  [8] Han H F, Wang Q, Liu X, et al. Chinese Journal of Chromatography (韩海峰, 王庆, 刘霞, 等. 色谱), 2012, 30(5): 538
9. [9]
  [9] Uckoo R M, Jayaprakasha G K, Nelson S D, et al. Talanta, 2011, 839: 48
10. [10]
  [10] Yang M H, Choong Y M. Food Chem, 2001, 75: 101
11. [11]
  [11] Koubaa M, Cocuron J C, Thomasset B, et al. Anal Biochem, 2013, 436: 151
12. [12]
  [12] Qureshi M S, Bhongale S S, Thorave A K. J Chromatogr A, 2011, 1218: 7147
13. [13]
  [13] Barros L, Pereira C, Ferreira I C F R. Food Anal Method, 2013, 6: 309
14. [14]
  [14] Ma R, Ouyang J, Li X, et al. Chinese Journal of Chromatography (马瑞, 欧阳嘉, 李鑫, 等. 色谱), 2012, 30(1): 62
15. [15]
  [15] Scherer R, Rybka A C P, Ballus C A, et al. Food Chem, 2012, 135: 150
16. [16]
  [16] Ehling S, Cole S. J Agric Food Chem, 2011, 59: 2229
17. [17]
  [17] Fu T, Liu Q S, Fan Z Y, et al. China Animal Husbandry and Veterinary Medicine (傅彤, 刘庆生, 范志影, 等. 中国畜牧兽医), 2005, 32(5): 16
18. [18]
  [18] Liu X, Zheng T, Shi C O, et al. China Feed (刘霞, 郑婷, 施超欧, 等. 中国饲料), 2009(8): 39
19. [19]
  [19] Zheng T, Cai Y X, Liu X, et al. Chinese Journal of Analysis Laboratory (郑婷, 蔡玉兴, 刘霞, 等. 分析试验室), 2009, 28(Suppl): 230
20. [20]
  [20] Losito I, Amorisco A, Carbonara T, et al. Anal Chim Acta, 2006, 575: 89
21. [21]
  [21] Dinh Q T, Alliot F, Moreau-Guigon E, et al. Talanta, 2011, 85: 1238
22. [22]
  [22] Niemann R A, Anderson D L. J Chromatogr A, 2008, 1200: 193
1. [1]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
2. [2]
  Aoyu Huang , Jun Xu , Yu Huang , Gui Chu , Mao Wang , Lili Wang , Yongqi Sun , Zhen Jiang , Xiaobo Zhu . Tailoring Electrode-Electrolyte Interfaces via a Simple Slurry Additive for Stable High-Voltage Lithium-Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(4): 100037-. doi: 10.3866/PKU.WHXB202408007
3. [3]
  Xinyu Zhu , Meili Pang . Application of Functional Group Addition Strategy in Organic Synthesis. University Chemistry, 2024, 39(3): 218-230. doi: 10.3866/PKU.DXHX202308106
4. [4]
  Xuejie Wang , Guoqing Cui , Congkai Wang , Yang Yang , Guiyuan Jiang , Chunming Xu . 碳基催化剂催化有机液体氢载体脱氢研究进展. Acta Physico-Chimica Sinica, 2025, 41(5): 100044-. doi: 10.1016/j.actphy.2024.100044
5. [5]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
6. [6]
  Wendian XIE , Yuehua LONG , Jianyang XIE , Liqun XING , Shixiong SHE , Yan YANG , Zhihao HUANG . Preparation and ion separation performance of oligoether chains enriched covalent organic framework membrane. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1528-1536. doi: 10.11862/CJIC.20240050
7. [7]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
8. [8]
  Zelong LIANG , Shijia QIN , Pengfei GUO , Hang XU , Bin ZHAO . Synthesis and electrocatalytic CO₂ reduction performance of metal-organic framework catalysts loaded with silver particles. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 165-173. doi: 10.11862/CJIC.20240409
9. [9]
  Ke Li , Chuang Liu , Jingping Li , Guohong Wang , Kai Wang . 钛酸铋/氮化碳无机有机复合S型异质结纯水光催化产过氧化氢. Acta Physico-Chimica Sinica, 2024, 40(11): 2403009-. doi: 10.3866/PKU.WHXB202403009
10. [10]
  Yukun Chang , Haoqin Huang , Baolei Wang . Preparation of Trans-Cinnamic Acid via “One-Pot” Protocol of Aldol Condensation-Hydrolysis Reaction: Recommending an Improved Organic Synthesis Experiment. University Chemistry, 2024, 39(4): 322-328. doi: 10.3866/PKU.DXHX202309095
11. [11]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
12. [12]
  Yuan Zheng , Quan Lan , Zhenggen Zha , Lingling Li , Jun Jiang , Pingping Zhu . Teaching Reform of Organic Synthesis Experiments by Introducing Reverse Thinking and Design Concepts: Taking the Synthesis of Cinnamic Acid Based on Retrosynthetic Analysis as an Example. University Chemistry, 2024, 39(6): 207-213. doi: 10.3866/PKU.DXHX202310065
13. [13]
  Wenxiu Yang , Jinfeng Zhang , Quanlong Xu , Yun Yang , Lijie Zhang . Bimetallic AuCu Alloy Decorated Covalent Organic Frameworks for Efficient Photocatalytic Hydrogen Production. Acta Physico-Chimica Sinica, 2024, 40(10): 2312014-. doi: 10.3866/PKU.WHXB202312014
14. [14]
  Aiai WANG , Lu ZHAO , Yunfeng BAI , Feng FENG . Research progress of bimetallic organic framework in tumor diagnosis and treatment. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1825-1839. doi: 10.11862/CJIC.20240225
15. [15]
  Feng Sha , Xinyan Wu , Ping Hu , Wenqing Zhang , Xiaoyang Luan , Yunfei Ma . Design of Course Ideology and Politics for the Comprehensive Organic Synthesis Experiment of Benzocaine. University Chemistry, 2024, 39(2): 110-115. doi: 10.3866/PKU.DXHX202307082
16. [16]
  Tianyun Chen , Ruilin Xiao , Xinsheng Gu , Yunyi Shao , Qiujun Lu . Synthesis, Crystal Structure, and Mechanoluminescence Properties of Lanthanide-Based Organometallic Complexes. University Chemistry, 2024, 39(5): 363-370. doi: 10.3866/PKU.DXHX202312017
17. [17]
  Shicheng Yan . Experimental Teaching Design for the Integration of Scientific Research and Teaching: A Case Study on Organic Electrooxidation. University Chemistry, 2024, 39(11): 350-358. doi: 10.12461/PKU.DXHX202408036
18. [18]
  Yong Wang , Yingying Zhao , Boshun Wan . Analysis of Organic Questions in the 37th Chinese Chemistry Olympiad (Preliminary). University Chemistry, 2024, 39(11): 406-416. doi: 10.12461/PKU.DXHX202403009
19. [19]
  Ran HUO , Zhaohui ZHANG , Xi SU , Long CHEN . Research progress on multivariate two dimensional conjugated metal organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2063-2074. doi: 10.11862/CJIC.20240195
20. [20]
  Bin HE , Hao ZHANG , Lin XU , Yanghe LIU , Feifan LANG , Jiandong PANG . Recent progress in multicomponent zirconium?based metal-organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2041-2062. doi: 10.11862/CJIC.20240161

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(265)
HTML views(7)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142