Citation: XIE Wen, SHI Yingzhu, HOU Jianbo, HUANG Chaoqun, ZHAO Dong, PAN Lulu, DONG Suozhuai. Simultaneous determination of ethephon, thidiazuron, diuron residues in cotton by using high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(2): 179-183. doi: 10.3724/SP.J.1123.2013.09052 shu

Simultaneous determination of ethephon, thidiazuron, diuron residues in cotton by using high performance liquid chromatography-tandem mass spectrometry

1.
1. Technical Center, Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, China;

Corresponding author: XIE Wen,
Received Date: 30 September 2013
Available Online: 29 November 2013
Fund Project: 国家公益性专项(201110041) (201110041)浙江出入境检验检疫局科研项目(ZK201344X). (ZK201344X)

A method for the determination of ethephon, thidiazuron and diuron in cotton samples has been developed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample was extracted with methanol-water. The separation was carried out on a C₈ column (150 mm×4.6 mm, 5 μm) with methanol-water (6:4, v/v) as mobile phase at a flow rate of 0.4 mL/min, and the injection volume was 20 μL. Then the sample solution was analyzed by HPLC-MS/MS in negative ion mode with multiple reaction monitoring (MRM). There were one precursor/two product ion transitions for each pesticide. The results showed that the working curves were linear in the range of 0-10 μg/L for ethephon and thidiazuron, and 0-1 μg/L for diuron. The correlation coefficients (r) were all over 0.999. The limits of quantitation (LOQ) of ethephon, diuron were 40 μg/kg, that of thidiazuron was 4 μg/kg. The average recoveries varied from 89.4% to 100.2% with the relative standard deviations (RSDs) of 5.7%-11.5% at three spiked levels (LOQ, 2LOQ and 4LOQ). The method is simple, rapid and accurate, and can meet the requirements of the domestic and international legislation. The method adapts to confirm the residues of ethephon, thidiazuron and diuron pesticides in cotton samples.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- ethephon,
- thidiazuron,
- diuron,
- cotton
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沈阳化工大学材料科学与工程学院沈阳 110142