Citation: SONG Wei, HU Yanyun, HAN Fang, LV Yaning, ZHENG Ping, ZHOU Fangfang, CHEN Xiaomei, QIU Jing, ZHOU Xiaoying. Simultaneous determination of clopidol, sulfonamide and quinolone residues in chickens by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(12): 1161-1166. doi: 10.3724/SP.J.1123.2013.08022 shu

Simultaneous determination of clopidol, sulfonamide and quinolone residues in chickens by ultra performance liquid chromatography-tandem mass spectrometry

SONG Wei ¹ ,
HU Yanyun ^1,2,, ,
HAN Fang ² ,
LV Yaning ² ,
ZHENG Ping ^1,2 ,
ZHOU Fangfang ³ ,
CHEN Xiaomei ¹ ,
QIU Jing ² ,
ZHOU Xiaoying ²

1.
1. Technical Center of Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230022, China;

Corresponding author: HU Yanyun,
Received Date: 13 August 2013
Available Online: 3 September 2013
Fund Project:

An analytical method for the simultaneous determination of clopidol, sulfadiazine, sulfamethazine, sulfametoxydiazine, sulfamethoxypyridazine, norfloxacin, ofloxacin, ciprofloxacin, enrofloxacin in chickens by ultra performance liquid chromatography coupled with tandem quadrupole mass spectrometry (UPLC-MS/MS) in positive ion mode with multiple reaction monitoring (MRM) has been developed and validated. The samples were homogenized and extracted with acetonitrile. After defatted with high speed frozen centrifugation, the supernatant solution was evaporated and the residue was dissolved with the mobile phase and defatted with n-hexane. It was then analyzed with UPLC-MS/MS. The limit of detection of this method was 0.1 μg/kg, and the limit of quantification was 0.5 μg/kg. The average recoveries (spiked at the levels of 0.5, 1.0, 2.0 μg/kg) ranged from 81.5% to 97.6%, with the relative standard deviations between 2.1% and 8.9%. The results demonstrated that the method is simple, accurate and suitable for the identification and quantification of these drug residues in chickens.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- clopidol,
- sulfonamides,
- quinolonens,
- chicken,
- multiresidue
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