Citation: PENG Shunü, WANG Qiuquan, FANG Lanlan, GUO Shanyong, ZENG Zhouhua, LIN Zhuguang. Simultaneous determination of nine preservatives in fruits using gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(1): 69-73. doi: 10.3724/SP.J.1123.2013.06035 shu

Simultaneous determination of nine preservatives in fruits using gas chromatography-mass spectrometry

1.
1. Department of Chemistry & Institute of Analytical Science, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;

Corresponding author: LIN Zhuguang,
Received Date: 19 June 2013
Available Online: 25 July 2013
Fund Project: 国家重点基础研究发展计划(“973”)项目(2009CB421605). (“973”)项目(2009CB421605)

A gas chromatography-mass spectrometry (GC-MS) method was established for the simultaneous determination of nine typical preservatives (pyrimethanil, chlorothalonil, chlorpyrifos, triadimefon, thiabendazole, imazalil, myclobutanil, iprodione, prochloraz) in fruits. The fruit samples were subjected to ultrasonic extraction with hexane/ethyl acetate (1/1, v/v), and followed by purification using diatomite column chromatography with hexane/ethyl acetate (1/3, v/v) eluant. Qualitative and quantitative analysis of the nine preservatives were performed on the GC-MS at full-scan (SCAN) and selected ion monitoring (SIM) modes, in which triphenylphosphate was used as the internal standard. The detection limits obtained for the nine preservatives were ranged from 0.10 μg/kg to 2.16 μg/kg. The average recoveries were in the range of 75.3% to 128% at the spiked levels of 50, 100 and 200 μg/kg with the relative standard deviations (RSDs) of 1.57% to 11.6% (n=5). The results showed that the developed method is sensitive and accurate for the determination of the nine preservatives in fruits.
- gas chromatography-mass spectrometry (GC-MS),
- preservatives,
- fruits,
- pesticide residue
1. [1]
  [1] Wu X H, Ai Q J, Xiao Y. Storage & Process (吴小虎, 艾启俊, 肖艺. 保鲜与加工), 2006(2): 4
2. [2]
  [2] Food Safe (食品安全网). (2013-06-05)[2013-07-20]. http://www. tbt-sps.gov.cn/foodsafe/xlbz/Pages/pesticide.aspx
3. [3]
  [3] GB 2763-2012
4. [4]
  [4] Lin H D, Zou Z F, Qin Y, et al. Chinese Journal of Chromatography (林海丹, 邹志飞, 秦燕, 等. 色谱), 2013, 31(2): 170
5. [5]
  [5] Hu B Z, Cai H J, Song W H. Chinese Journal of Chromatography (胡贝贞, 蔡海江, 宋伟华. 色谱), 2012, 30(9): 889
6. [6]
  [6] Xu G F, Nie J Y, Li J, et al. Chinese Journal of Pesticide Science (徐国锋, 聂继云, 李静, 等. 农药学学报), 2009, 11(3): 351
7. [7]
  [7] Chen D J, Zhang Z G, Zhou Y, et al. Chinese Journal of Chromatography (陈达捷, 张志刚, 周昱, 等. 色谱), 2012, 30(9): 896
8. [8]
  [8] Shen J, Li M, Ibraim A, et al. Chinese Journal of Pharmaceutical Analysis (沈静, 李明, 艾尔肯·依不拉音, 等. 药物分析杂志), 2007, 27(6): 921
9. [9]
  [9] Shen W J, Lin H, Zhao Z Y, et al. Chinese Journal of Chromatography (沈伟健, 林宏, 赵增运, 等. 色谱), 2008, 26(4): 515
10. [10]
  [10] Fróes M B R, Santos L F S, Navickiene S. Food Anal Methods, 2013, 6: 328
11. [11]
  [11] Li X Q, Ji C, Sun Y Y, et al. Food Chem, 2009, 113: 692
12. [12]
  [12] Nie J Y, Li J, Li H F, et al. Plant Protection (聂继云, 李静, 李海飞, 等. 植物保护), 2007, 33(6): 26
1. [1]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
2. [2]
  Yahui Ma , Leiyang Lv . Pears on the Journey of Fruits. University Chemistry, 2024, 39(9): 64-73. doi: 10.12461/PKU.DXHX202404029
3. [3]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
4. [4]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
5. [5]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
6. [6]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
7. [7]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
8. [8]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
9. [9]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
10. [10]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
11. [11]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064
12. [12]
  Yuanchun Pan , Xinyun Lin , Leyi Yang , Wenya Hu , Dekui Song , Nan Liu . Artificial Intelligence Science Practice: Preparation of Electronic Skin by Chemical Vapor Deposition of Graphene. University Chemistry, 2025, 40(11): 272-280. doi: 10.12461/PKU.DXHX202412052
13. [13]
  Xichen YAO , Shuxian WANG , Yun WANG , Cheng WANG , Chuang ZHANG . Oxygen reduction performance of self?supported Fe/N/C three-dimensional aerogel catalyst layers. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1387-1396. doi: 10.11862/CJIC.20240384
14. [14]
  Hailang JIA , Yujie LU , Pengcheng JI . Preparation and properties of nitrogen and phosphorus co-doped graphene carbon aerogel supported ruthenium electrocatalyst for hydrogen evolution reaction. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2327-2336. doi: 10.11862/CJIC.20250021
15. [15]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
16. [16]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
17. [17]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
18. [18]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
19. [19]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
20. [20]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(524)
HTML views(5)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142