Citation: MOU Yanli, GUO Dehua, DING Zhuoping. Determination of four plant growth regulator residues in amphisarcas by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(10): 1016-1020. doi: 10.3724/SP.J.1123.2013.06003 shu

Determination of four plant growth regulator residues in amphisarcas by high performance liquid chromatography-tandem mass spectrometry

MOU Yanli ¹ ,
GUO Dehua ^2,, ,
DING Zhuoping ^1,,

1.
1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;

Corresponding author: GUO Dehua, DING Zhuoping,
Received Date: 3 June 2013
Available Online: 2 July 2013

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of the plant growth regulator (PGR) (paclobutrazol, forchlorfenuron, isopentennyladenine and 6-benzylaminopurine) residues in amphisarcas. The sample was extracted with acetonitrile, then cleaned up by MCX solid phase extraction. The HPLC separation was performed on an Agilent XDB-C₁₈ column with 5 mmol/L ammonium acetate solution and acetonitrile containing 0.1% (volume percentage) formic acid as the mobile phases in a gradient elution mode. The PGRs were determined by MS/MS in positive electrospray ionization mode, and quantified by matrix-matched external standard method. The calibration curves showed good linearity in each range with correlation coefficients greater than 0.999. The limits of quantification (LOQs, S/N>10) were 0.04-1.35 μg/kg and the limits of detection (LODs, S/N>3) were 0.01-0.41 μg/kg for the four PGRs spiked in cucumber and apple. The recoveries of the four PGRs spiked at three levels ranged from 81.0% to 93.3%, with the relative standard deviations (RSDs) of 3.5%-9.5%. The sensitivity, accuracy and precision of the method meet the technical standards of the pesticide determination. Therefore the method can be applied to the determination of the four PGRs in amphisarcas.
1. [1]
  [1] Song Y, Zhang Y H, Huang X, et al. Chinese Journal of Analytical Chemistry (宋莹, 张耀海, 黄霞, 等. 分析化学), 2011, 39(8): 1270
2. [2]
  [2] Zhou Y F, Zhang S R. Pesticides (周永丰, 张寿儒. 农药), 2003, 42(11): 43
3. [3]
  [3] Zhao D Y, Li M, Yu J L, et al. Pesticides (赵德友, 李美, 于建垒, 等. 农药), 2000, 39(12): 39
4. [4]
  [4] Zhang J, Wang L L, Liu H Y, et al. Chinese Journal of Analytical Chemistry (张洁, 王琳琳, 刘海燕, 等. 分析化学), 2010, 38(1): 147
5. [5]
  [5] Jin M C, Chen X H, Li X P, et al. Analytical Instrumentation (金米聪, 陈晓红, 李小平, 等. 分析仪器), 2005(3): 29
6. [6]
  [6] WTO/TBT-SPS Notification and Enquiry of China. Maximum Residue Limits Inquiry (中华人民共和国WTO/TBT-SPS国家通报咨询中心. 最大残留限量查询). (2012-08-20) [2012-12-02]. http://www.tbt-sps.gov.cn/jpmrl/MRLS/Pages/MRLSsearch.aspx
7. [7]
  [7] Editorial Committee of Maximum Residue Limits of Chemical Residues in Food. Maximum Residue Limits of Chemical Residues in Food. Beijing: China Standards Press (《食品中农业化学品残留限量》编委会. 食品中农业化学品残留限量. 北京: 中国标准出版社), 2006: 44-47, 53-62, 684-685, 700-703, 937
8. [8]
  [8] United States Department of Agriculture (FAS). Pesticide MRL Database (frequently). [2012-12-02]. http://www.mrldatabase.com
9. [9]
  [9] GB 2760-1996
10. [10]
  [10] Qi M F. Jiangsu Agricultural Sciences (齐明芳. 江苏农业科学), 2011, 39(6): 486
11. [11]
  [11] Lu Y M, Yi G B, Chen C B, et al. Journal of Instrumental Analysis (陆益民, 易国斌, 陈创彬, 等. 分析测试学报), 2011, 30(2): 186
12. [12]
  [12] Wang W. Agrochemicals (汪伟. 农药), 2009, 48(2): 116
13. [13]
  [13] Xia M, An L T, Yi Q F, et al. Chemical Reagents (夏敏, 安礼涛, 尹起范, 等. 化学试剂), 2009, 31(11): 913
14. [14]
  [14] Xu Y, Shou L F, Yu M, et al. Chinese Journal of Pesticide Science (徐永, 寿林飞, 虞淼, 等. 农药学学报), 2012, 14(1): 61
15. [15]
  [15] Kaushik B, Dasharath P O, Soma D, et al. J Chromatogr A, 2007, 1173(1/2): 98
16. [16]
  [16] Yu H, Lu Y, Wang J J, et al. Xinjiang Agricultural Sciences (于红, 鹿毅, 王静静, 等. 新疆农业科学), 2011, 48(1): 187
17. [17]
  [17] Camino-Sánchez F J, Zafra-Gómez A, Ruiz-García J, et al. J Food Compos Anal, 2011, 24(3): 427
18. [18]
  [18] Lü Y, Zhao J, Yang T, et al. China Measurement & Testing Technology (吕燕, 赵健, 杨挺, 等. 中国测试), 2010, 36(4): 57
19. [19]
  [19] Hogenboom A C, Hofman M P, Kok S J, et al. J Chromatogr A, 2000, 892(1/2): 379
20. [20]
  [20] Hu J T, Sheng Y, Fang Z, et al. Chinese Journal of Chromatography (胡江涛, 盛毅, 方智, 等. 色谱), 2007, 25(3): 441
21. [21]
  [21] Liu J J, Gong P, Zhang X M, et al. Chinese Journal of Chromatography (刘靖靖, 宫萍, 张晓梅, 等. 色谱), 2012, 30(10): 1012
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
4. [4]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
5. [5]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
6. [6]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
7. [7]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
8. [8]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065
9. [9]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
10. [10]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
11. [11]
  Haiyang Zhang , Yanzhao Dong , Haojie Li , Ruili Guo , Zhicheng Zhang , Jiangjiexing Wu . Exploring the Integration of Chemical Engineering Principle Experiment with Cutting-Edge Research Achievements. University Chemistry, 2024, 39(10): 308-313. doi: 10.12461/PKU.DXHX202405035
12. [12]
  Lihui Jiang , Wanrong Dong , Hua Yang , Yongqing Xia , Hongjian Peng , Jun Yuan , Xiaoqian Hu , Zihan Zeng , Yingping Zou , Yiming Luo . Study on Extraction of p-Hydroxyacetophenone. University Chemistry, 2024, 39(11): 259-268. doi: 10.12461/PKU.DXHX202402056
13. [13]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
14. [14]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
15. [15]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
16. [16]
  Qin Li , Huihui Zhang , Huajun Gu , Yuanyuan Cui , Ruihua Gao , Wei-Lin Dai . In situ Growth of Cd_0.5Zn_0.5S Nanorods on Ti₃C₂ MXene Nanosheet for Efficient Visible-Light-Driven Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2025, 41(4): 100031-. doi: 10.3866/PKU.WHXB202402016
17. [17]
  Aoyu Huang , Jun Xu , Yu Huang , Gui Chu , Mao Wang , Lili Wang , Yongqi Sun , Zhen Jiang , Xiaobo Zhu . Tailoring Electrode-Electrolyte Interfaces via a Simple Slurry Additive for Stable High-Voltage Lithium-Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(4): 100037-. doi: 10.3866/PKU.WHXB202408007
18. [18]
  Chunyang Zheng , Shiyu Liu , Nuo Yi , Hong Shang . The Adventures in the Kingdom of Plant Pigments. University Chemistry, 2024, 39(9): 170-176. doi: 10.3866/PKU.DXHX202308085
19. [19]
  Asif Hassan Raza , Shumail Farhan , Zhixian Yu , Yan Wu . 用于高效制氢的双S型ZnS/ZnO/CdS异质结构光催化剂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406020-. doi: 10.3866/PKU.WHXB202406020
20. [20]
  Qingqing SHEN , Xiangbowen DU , Kaicheng QIAN , Zhikang JIN , Zheng FANG , Tong WEI , Renhong LI . Self-supporting Cu/α-FeOOH/foam nickel composite catalyst for efficient hydrogen production by coupling methanol oxidation and water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1953-1964. doi: 10.11862/CJIC.20240028

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(176)
HTML views(12)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142