Citation: WANG Yong, GAO Peifeng, ZHAO Xinying, QU Feng. Characterization of stability of polypeptides and glycoproteins by capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2013, 31(6): 543-549. doi: 10.3724/SP.J.1123.2013.03052 shu

Characterization of stability of polypeptides and glycoproteins by capillary electrophoresis

WANG Yong ¹ ,
GAO Peifeng ^1,, ,
ZHAO Xinying ^1,2 ,
QU Feng ¹

1.
1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China;

Corresponding author: GAO Peifeng,
Received Date: 29 March 2013
Available Online: 25 April 2013
Fund Project: 国家自然科学基金项目(21175011,20875009) (21175011,20875009)科技部基础研究计划项目(2012CB910603). (2012CB910603)

A capillary electrophoresis (CE) method was developed for the stability characterization of polypeptides and glycoproteins. Angiotensin II (Ang II), phytagglutinin (PHA), bovine thrombin (B-Thr), human thrombin (H-Thr) and horseradish peroxidase (HRP) were used as polypeptide and glycoprotein mode molecules. The parameters affecting the analysis efficiency for Ang II, such as sample concentration, running buffer, pH and ionic strength of sample solution were optimized, as for the glycoprotein, capillary conditions, charge state of sample, running buffer and applied voltage were optimized. It showed that the Ang II was stable when kept in borate buffer (0.02 mol/L pH 7.4) at 4℃ for 48 h. The four glycoproteins were quite stable in borate buffer (0.2 mol/L pH 7.4) at 20, 4, -20℃ for 48 h, and also kept stable at -20℃ when deposited over one week and less than four weeks. HRP was the only one that kept stable when deposited over two weeks and less than four weeks. This method is effective, rapid, simple and low-cost and can be widely used for the stability characterization of polypeptides and glycoproteins.
- capillary electrophoresis (CE),
- polypeptide,
- glycoprotein,
- stability
1. [1]
  [1] Basso N, Terragno N A. Hypertension, 2001, 38(6): 1246
2. [2]
  [2] Tonnaer J A, Verhoef J, Wiegant V M, et al. J Chromatogr B, 1980, 183(3): 303
3. [3]
  [3] Ahmad S, Simmons T, Varagic J, et al. PLoS One, 2011, 6(12): e28501
4. [4]
  [4] Zhou X, Wang X J, Tian H S, et al. Journal of Jilin University: Medicine Edition (周鑫, 王晓杰, 田海山, 等. 吉林大学学报), 2010, 36(3): 518
5. [5]
  [5] Tenorio-López F A, Zarco-Olvera G, Sánchez-Mendoza A, et al. Talanta, 2010, 80(5): 1702
6. [6]
  [6] Huang Z D. Chinese Journal of Chromatography (黄志东. 色谱), 2000, 18(1): 80
7. [7]
  [7] Wojcik R, Dada O O, Sadilek M, et al. Rapid Commun Mass Spectrom, 2010, 24(17): 2554
8. [8]
  [8] Kuroda Y, Yukinaga H, Kitano M, et al. J Pharm Biomed Anal, 2005, 37(3): 423
9. [9]
  [9] Hasan M N, Park S H, Oh E, et al. J Sep Sci, 2010, 33(23/24): 3701
10. [10]
  [10] Chen T H, Misra T K, Liu C Y. Electrophoresis, 2008, 29(8): 1651
11. [11]
  [11] Wang X L, Kumar S, Buck P M, et al. Proteins, 2013, 83(3): 443
12. [12]
  [12] Lazaridis T, Karplus M. Biophysical Chemistry, 2003, 100(1/3): 367
13. [13]
  [13] Scharnagl C, Reif M, Friedrich J. Biochimica et Biophysica Acta, 2005, 1749(2): 187
14. [14]
  [14] Wu S H, Luo T S, Zhang C G, et al. Chinese Journal of Experimental Traditional Medical Formulae (吴少辉, 罗廷顺, 张成桂, 等. 中国实验方剂学杂志), 2012, 18(21): 74
15. [15]
  [15] Kakehi K, Honda S. J Chromatogr A, 1996, 720(1/2): 377
16. [16]
  [16] Yin L H, Xue J, Lin B C. Chinese Journal of Analytical Chemistry (尹利辉, 薛俊, 林秉承. 分析化学), 1996, 24(12): 1464
17. [17]
  [17] Moring S E, Colburn J C, Grossman P D, et al. LC-GC, 1990, 8(1): 34
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