Citation: CHEN Xiaozhen, CHEN Wanqin, WANG Jin, HUANG Liying, ZHANG Donglei. Determination of dicyandiamide in dairy products by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(9): 875-877. doi: 10.3724/SP.J.1123.2013.03014 shu

Determination of dicyandiamide in dairy products by high performance liquid chromatography

1.
Zhejiang Institute of Quality Inspection Science, Key Laboratory for Food Safety of Zhejiang Province, Hangzhou 310013, China

Corresponding author: CHEN Xiaozhen,
Received Date: 5 March 2013
Available Online: 22 April 2013
Fund Project: 浙江省质量技术监督系统科研计划项目(20120205). (20120205)

A high performance liquid chromatographic (HPLC) method for the determination of dicyandiamide in dairy products was developed. The sample was extracted by acetonitrile, separated on an XBridge Amide column (250 mm×4.6 mm, 3.5 μm) using acetonitrile-water (90/10, v/v) containing 0.2%(v/v) formic acid as the mobile phase at a flow rate of 1.0 mL/min, and determined by a photodiode array detector at the wavelength of 218 nm. The calibration curve was linear in the range of 0.5-50 mg/L with the correlation coefficient (r²) of 0.9999. The recoveries were from 96.7% to 101.0% with the relative standard deviations (RSDs) in the range of 4.5% to 4.9%(n=6). The limit of detection was 0.2 mg/kg, and the limit of quantification was 0.5 mg/kg. The method is simple, sensitive, accurate and precise for the determination of dicyandiamide in dairy products.
- high performance liquid chromatography (HPLC),
- dicyandiamide,
- dairy products
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沈阳化工大学材料科学与工程学院沈阳 110142