Citation: ZHAO Yanyan, LIU Liyan, HAN Yuanyuan, LI Yueqiu, WANG Yan, SHI Minjian. Simultaneous determination of principal components and related substances of raw material drug of ammonium glycyrrhizinate by reversed-phase high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(9): 869-874. doi: 10.3724/SP.J.1123.2013.03004 shu

Simultaneous determination of principal components and related substances of raw material drug of ammonium glycyrrhizinate by reversed-phase high performance liquid chromatography

ZHAO Yanyan ^1,2,, ,
LIU Liyan ¹ ,
HAN Yuanyuan ¹ ,
LI Yueqiu ¹ ,
WANG Yan ² ,
SHI Minjian ²

1.
1. Medical Experimental Center, Hebei University, Baoding 071000, China;

Corresponding author: ZHAO Yanyan,
Received Date: 1 March 2013
Available Online: 18 April 2013
Fund Project: 河北省自然科学基金项目(H2013201203) (H2013201203)河北省教育厅科学研究计划项目(2009315) (2009315)河北省卫生厅医学科学研究重点课题计划项目(20090570) (20090570)河北省中医药管理局科研计划课题(2008072). (2008072)

An analytical method for the simultaneous determination of 18α-glycyrrhizic acid, 18β-glycyrrhizinic acid, related substances A and B and drug quality standard by reversed-phase high performance liquid chromatography (RP-HPLC) was established. The assay was carried out on a Durashell-C₁₈ column (250 mm×4.6 mm, 5 μm) with 10 mmol/L ammonium perchlorate (the pH value was adjusted to 8.20 with ammonia)-methanol (48:52, v/v) as mobile phase at a flow rate of 0.80 mL/min, and the detection wavelength was set at 254 nm. The column temperature was 50 ℃ and the injection volume was 10 μL. Under the separation conditions, the calibration curves of the analytes showed good linearities within the mass concentrations of 0.50-100 mg/L (r>0.9999). The detection limits for 18α-glycyrrhizic acid, 18β-glycyrrhizinic acid, related substances A and B were 0.15, 0.10, 0.10, 0.15 mg/L, respectively. The average recoveries were between 97.32% and 99.33% (n=3) with the relative standard deviations (RSDs) between 0.05% and 1.06%. The method is sensitive, reproducible, and the results are accurate and reliable. The method can be used for the determination of principal components and related substances of ammonium glycyrrhizinate for the quality control of raw material drug of ammonium glycyrrhizinate.
- reversed-phase high performance liquid chromatography (RP-HPLC),
- ammonium glycyrrhizinate,
- principal component,
- related substances,
- raw material drug
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