Citation: LI Xue, MU Guangqing, CHEN Lijun, JIANG Tiemin. Determination of environmental estrogens in cow feed and soil by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(9): 908-913. doi: 10.3724/SP.J.1123.2013.02033 shu

Determination of environmental estrogens in cow feed and soil by high performance liquid chromatography-tandem mass spectrometry

LI Xue ^1,2 ,
MU Guangqing ¹ ,
CHEN Lijun ^1,2,, ,
JIANG Tiemin ²

1.
1. College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China;

Corresponding author: CHEN Lijun,
Received Date: 27 February 2013
Available Online: 24 April 2013
Fund Project: 国家"863"计划项目(2011AA100903) (2011AA100903)

An analytical method was developed for the determination of the residues of five environmental estrogens, including estriol, estradiol, estrone, bisphenol A and diethylstilbestrol, in the cow feed and soil by solid phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The important parameters which affect the determination efficiency such as the mobile phase, the condition of mass spectrometry and solid phase extraction column were optimized. The optimal determination conditions were as follows: the sample was extracted with acetonitrile at first, then cleaned-up with an NH₂-SPE column, and an Acquity UPLC^TM HSS T₃ column was selected to separate the analytes. Acetonitrile-methanol (4:1, v/v) and 0.01% ammonia aqueous solution were used as the mobile phase by gradient elution in the negative mode. The limits of detection (LOD, S/N=3) were 0.06-0.22 μg/kg. The overall recoveries varied from 81.70% to 102.20%, and the relative standard deviations (RSDs) were all less than 10.00%. This method is simple, sensitive and accurate, and suitable for the determination of environmental estrogens in cow feed and soil.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- environmental estrogens,
- cow feed,
- soil
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