Citation: ZHANG Lin, ZHANG Fucheng, WANG Zhaohong, JIANG Ye, XU Meng, LI Hong. Simultaneous determination of ephedrine and N-methylephedrine in urine by solid phase extraction-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(9): 898-902. doi: 10.3724/SP.J.1123.2013.02028 shu

Simultaneous determination of ephedrine and N-methylephedrine in urine by solid phase extraction-ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry

ZHANG Lin ¹ ,
ZHANG Fucheng ² ,
WANG Zhaohong ³ ,
JIANG Ye ^1,, ,
XU Meng ¹ ,
LI Hong ⁴

1.
1. School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China;

Corresponding author: JIANG Ye,
Received Date: 25 February 2013
Available Online: 14 April 2013
Fund Project: 河北省卫生厅科研基金项目(08056). (08056)

A rapid and sensitive method has been developed for the simultaneous determination of ephedrine and N-methylephedrine in urine samples by solid phase extraction-ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (SPE-UPLC-ESI MS/MS). The samples were extracted with Oasis MCX solid phase extraction cartridges and measured in the modes of electrospray positive ionization (ESI+) and multiple reaction monitoring (MRM). Good linearities were observed in the range of 0.0250-2.50 μg/L with correlation coefficient over 0.9990 for both analytes. The recoveries were above 80% with RSDs less than 5.0%. The limits of detection were 0.01 μg/L. The method proves to be rapid and sensitive for the trace determination of ephedrine and N-methylephedrine in urine samples.
1. [1]
  [1] Abourashed E A, El-Alfy A T, Khan I A, et al. Phytother Res, 2003, 17: 703
2. [2]
  [2] Li K, Chen J, Xu X, et al. Chinese Journal of Pharmaceuticals (李坤, 陈钧, 徐新, 等. 中国医药工业杂志), 2009, 40 (9): 695
3. [3]
  [3] Feng Y, Huang Y, Lai W L, et al. Journal of Guangdong College of Pharmacy (冯怡, 黄羽, 赖伟玲, 等. 广东药学院学报), 2008, 24(6): 572
4. [4]
  [4] Ren L, Deng X X, Bi K S, et al. Chinese Journal of Pharmaceutical Analysis (任磊, 邓新秀, 毕开顺, 等. 药物分析杂志), 2007, 27(10): 1544
5. [5]
  [5] Liu Q Y, Zhang Z H, Chen B, et al. Journal of Instrumental Analysis (刘庆艳, 张朝辉, 陈波, 等. 分析测试学报), 2008, 27(1): 26
6. [6]
  [6] Li S G, Li H Y, Cai Z, et al. Chinese Journal of Analytical Chemistry (李斯光, 李海燕, 蔡卓, 等. 分析化学), 2009, 37(8): 1137
7. [7]
  [7] Wang L L, Fang Y, Ge W H. China Pharmacy (王璐璐, 方芸, 葛卫红. 中国药房), 2007, 18(32): 2510
8. [8]
  [8] Hu Z, Zou Q, Tian J, et al. J Chromatogr B, 2011, 879: 3937
9. [9]
  [9] Wang P Y, Zhang H M, Wang C F, et al. Chinese Journal of Information on TCM (王朋义, 张海鸣, 王成芳, 等. 中国中医药信息杂志), 2012, 19(1): 56
10. [10]
  [10] Zheng Z, Yan T, Chen W, et al. Xenobiotica, 2012, 42(8): 775
11. [11]
  [11] Salam R A A, Hadad G M, Hameed E A A. J Liq Chromatogr Relat Technol, 2013, 36: 384
12. [12]
  [12] Chen X, Bai X H, Wang X, et al. Chinese Journal of Chromatography (陈璇, 白小红, 王晓, 等. 色谱), 2010, 28(12): 1144
13. [13]
  [13] LeBelle M J, Savard C, Dawson B A, et al. Forensic Science International, 1995, 71: 215
14. [14]
  [14] Herráez-Hernández R, Campíns-Falcó P. J Chromatogr A, 2000, 893: 69
15. [15]
  [15] Aymard G, Labarthe B, Warot D, et al. J Chromatogr B, 2000, 744: 25
16. [16]
  [16] Sorensen L K. J Chromatogr B, 2011, 879: 727
17. [17]
  [17] Chen X H, Li X P, Yao X P. Chinese Journal of Health Laboratory Technology (陈晓红, 李小平, 姚浔平. 中国卫生检验杂志), 2006, 16(6): 691
18. [18]
  [18] He Y N, Hu Y Q, Yang H Y, et al. China Pharmacy Journal (何颖娜, 胡玉钦, 杨汉煜, 等. 中国药学杂志), 2009, 44(6): 464
19. [19]
  [19] Chen X H, Qiu P H, Jin M C et al. Physical Testing and Chemical Analysis Part B: Chemical Analysis (陈晓红, 仇佩虹, 金米聪, 等. 理化检验: 化学分册), 2006, 42(10): 790
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