Citation: WANG Jing, WANG Dan, ZHANG Hua, PENG Xiaojun, DONG Junpu. Analysis of docosahexenoic acid in human blood using heterocyclic derivatization-gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(8): 734-738. doi: 10.3724/SP.J.1123.2013.01050 shu

Analysis of docosahexenoic acid in human blood using heterocyclic derivatization-gas chromatography-triple quadrupole mass spectrometry

WANG Jing ¹ ,
WANG Dan ² ,
ZHANG Hua ^1,, ,
PENG Xiaojun ¹ ,
DONG Junpu ²

1.
1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China;

Corresponding author: ZHANG Hua,
Received Date: 30 January 2013
Available Online: 17 March 2013

A method was developed and validated for the analysis of docosahexenoic acid (DHA) in human blood by heterocyclic derivatization-gas chromatography coupled with triple quadrupole mass spectrometry (GC-MS/MS). 2-Amino-2-methyl-1-propanol (AMP) was used as the reaction reagent of DHA heterocyclic derivatization and the most optimal reaction conditions of this reaction were optimized. Multiple reaction monitoring and internal standard calibration curve were applied to detect DHA by GC-MS/MS. The linear range for the determination of DHA was 0.07-10 μg/mL (r²=0.9991). The limit of detection (S/N=2.8) was 0.02 μg/mL and the limit of quantification (S/N=10) was 0.07 μg/mL. The average recoveries of DHA at three spiked levels of 0.5, 1.5 and 2.5 μg ranged from 94.40% to 103.13% and the relative standard deviations (RSD) were in the range of 1.51%-3.16%. The method was simple, accurate, reliable and small amount of sample was required. It was suitable for detecting the contents of DHA in human blood.
1. [1]
  [1] Zhu L N, Zhang Z G, Zhang M, et al. China Dairy Industry (朱丽娜, 张志国, 张敏, 等. 中国乳品工业), 2009, 37(2): 45
2. [2]
  [2] Wang Y N, Hou D Y, Xin G, et al. Journal of Anshan Normal University (王月囡, 侯冬岩, 辛广, 等. 鞍山师范学院学报), 2012, 14(4): 50
3. [3]
  [3] Luo H, Huang B, Wang J, et al. Nursing Practice and Research (罗红, 黄冰, 王静, 等. 护理实践与研究), 2011, 8(2): 73
4. [4]
  [4] Guo Y H, Li Y J. Meat Research (郭玉华, 李钰金. 肉类研究), 2011(1): 82
5. [5]
  [5] National Postdoctoral Workstation Group of Xiuzheng Pharmaceutical Group Co. Chinese Journal of Healthy Birth & Child Care (修正国家级博士后工作站专家组. 中国优生优育), 2010, 16(4): 219
6. [6]
  [6] Cao W X, Meng J, Tian Y X. China Oils and Fats (曹万新, 孟橘, 田玉霞. 中国油脂), 2011, 36(3): 1
7. [7]
  [7] Bailey-Hall E, Nelson E B, Ryan A S. Lipids, 2008, 43(2): 181
8. [8]
  [8] Petrovic M, Kezic N, Bolanca V. Food Chemistry, 2010, 122(1): 285
9. [9]
  [9] Kangania C O, Kelleyb D E, DeLanya J P. J Chromatogr B, 2008, 873(1): 95
10. [10]
  [10] Tang S, Qin C, Wang H, et al. J Supercrit Fluids, 2011, 57(1): 44
11. [11]
  [11] Loukasa V, Dimizasa C, Sinanogloub V J, et al. Chem Phys Lipids, 2010, 163(3): 292
12. [12]
  [12] Bo H B, Wang X, Zhai Z D, et al. Chinese Journal of Chromatography (薄海波, 王霞, 翟宗德, 等. 色谱), 2006, 24(2): 181
13. [13]
  [13] Kou X Y, Yu G P. Food Research and Development (寇秀颖, 于国萍. 食品研究与开发), 2005, 26(2): 46
14. [14]
  [14] Li Q C, Zhang C, Peng H M, et al. Acta Pharmaceutica Sinica (李青翠, 张驰, 彭会明, 等. 药学学报), 1997, 32(8): 635
15. [15]
  [15] Xu H, Pan C P. Modem Scientific Instruments (许华, 潘灿平. 现代科学仪器), 2009(2): 116
16. [16]
  [16] Xu H Z, Zhang W L, Chen R G. Acta Nutrimenta Sinica (徐惠珠, 张伟利, 陈瑞冠. 营养学报), 1986, 8(2): 165
17. [17]
  [17] Li Y Y, Zhuang H Q, Mo L E, et al. Journal of Guangdong Medical College (黎运源, 庄海旗, 莫丽儿, 等. 广东医学院学报), 1994, 12(2): 90
18. [18]
  [18] Sun S L, Gu X H, Tang J, et al. Journal of Food Science and Biotechnology (孙素玲, 顾晓红, 汤坚, 等. 食品与生物技术学报), 2005, 24(2): 107
19. [19]
  [19] Zou Y H. Food Science (邹耀洪. 食品科学), 2007, 28(6): 293
20. [20]
  [20] Zhuang W, McKague B, Reeve D. Int J Mass Spectrom, 2004, 232(2): 127
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