Citation: YUN Huan, CUI Fengyun, YAN Hua, LIU Xin, HE Yue, ZHANG Zhaohui. Determination of ribavirin and amantadine in chicken by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(8): 724-728. doi: 10.3724/SP.J.1123.2013.01023 shu

Determination of ribavirin and amantadine in chicken by ultra performance liquid chromatography-tandem mass spectrometry

1.
Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China

Corresponding author: YUN Huan,
Received Date: 10 January 2013
Available Online: 11 March 2013
Fund Project: 国家质检总局科研项目(2013IK146). (2013IK146)

A method for the determination of ribavirin and amantadine in chicken has been developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After extracted by 1% (volume percentage) trichloroacetic acid solution-acetonitrile (1:1, v/v) and purified by a Supelco LC-SCX cartridge, the samples were loaded onto an Acquity UPLC BEH Hillic column (150 mm×2.1 mm, 1.7 μm)and separated with gradient elution. The electrospray was operated in the positive mode and the samples were monitored by the multiple reaction monitoring (MRM) mode. The limits of quantification (LOQs, S/N=10) of ribavirin and amantadine were 4.0 μg/kg. The calibration curves showed good linearity in the range of 10.0-100.0 μg/L, and the correlation coefficients (r²) were not lower than 0.99. When the spiked levels were 4.0, 8.0 and 20.0 μg/kg, the recoveries of ribavirin and amantadine in chicken ranged from 78% to 102.5%, with the relative standard deviations (RSDs) of 2.2%-7.6%. The results indicate that the method is simple, rapid, sensitive and suitable for the qualitative and quantitative analyses of ribavirin and amantadine in chicken samples.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- ribavirin,
- amantadine,
- chicken
1. [1]
  [1] Austin R K, Trefts P E, Hintz M. Antimicrob Agents Chemother, 1983, 24(5): 696
2. [2]
  [2] Roboz J, Suzuki R. J Chromatogr, 1978, 160: 169
3. [3]
  [3] Homma M, Jayewardene A L, Gambertoglio J, et al. Antimicrob Agents Chemother, 1999, 43(11): 2716
4. [4]
  [4] Cui C F, Chen C H, Lin H D, et al. Chinese Journal of Veterinary Drug (崔成富, 陈创华, 林海丹, 等. 中国兽药杂志), 2011, 45(9): 32
5. [5]
  [5] Page T, Connor J D. Int J Biochem, 1990, 22: 379
6. [6]
  [6] Yeh L T, Nguyen M, Lourenco D, et al. J Pharm Biomed Anal, 2005, 38: 34
7. [7]
  [7] Yeh L T, Nguyen M, Dadgostari S, et al. J Pharm Biomed Anal, 2007, 43: 1057
8. [8]
  [8] Zhu Y L, Shao D J, Jiang T M, et al. Chinese Journal of Veterinary Drug (朱永林, 邵德佳, 蒋天梅, 等. 中国兽药杂志), 2008, 42(7): 22
9. [9]
  [9] Qiang X Y, Zhu J B, Xu M, et al. Progress in Pharmaceutical Sciences (强晓妍, 朱监宝, 徐敏, 等. 药学进展), 2012, 36(9): 413
10. [10]
  [10] Zhu S P, Zhong H, He F, et al. Central South Pharmacy (朱胜平, 钟华, 何飞, 等. 中南药学), 2005, 3(4): 209
11. [11]
  [11] Lou Y J, Chen S, Tao Q F. Chin J Pharm Anal (楼永军, 陈爽, 陶巧凤. 药物分析杂志), 2011, 31(3): 493
12. [12]
  [12] Yun H, Zhang Z H, Luo S L, et al. Modern Instruments (云环, 张朝晖, 罗生亮, 等. 现代仪器), 2009, 15(85): 42
13. [13]
  [13] Liu Z C, Yang F, Yu K J, et al. Chinese Journal of Chromatography (刘正才, 杨方, 余孔捷, 等. 色谱), 2012, 30(12): 1253
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
4. [4]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
5. [5]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
6. [6]
  Qin Li , Ziyao Jia , Ye Chen , Mingze Ma , Lin Li , Tao Huang . A Journey into the Enigmatic World of Pickering Emulsion: A Chemical Science Popularization Experiment. University Chemistry, 2024, 39(9): 311-318. doi: 10.3866/PKU.DXHX202306035
7. [7]
  Tingting Yu , Si Chen , Lianglong Sun , Tongtong Shi , Kai Sun , Xin Wang . Comprehensive Experimental Design for the Photochemical Synthesis, Analysis, and Characterization of Difluoropyrroles. University Chemistry, 2024, 39(11): 196-203. doi: 10.3866/PKU.DXHX202401022
8. [8]
  Xiuyun Wang , Jiashuo Cheng , Yiming Wang , Haoyu Wu , Yan Su , Yuzhuo Gao , Xiaoyu Liu , Mingyu Zhao , Chunyan Wang , Miao Cui , Wenfeng Jiang . Improvement of Sodium Ferric Ethylenediaminetetraacetate (NaFeEDTA) Iron Supplement Preparation Experiment. University Chemistry, 2024, 39(2): 340-346. doi: 10.3866/PKU.DXHX202308067
9. [9]
  . CCS Chemistry | 国家自然科学基金委员会高飞雪&杨俊林：国家自然科学基金化学基础研究的资助策略、趋势与前沿. CCS Chemistry, 2025, 7(10.31635/ccschem.025.202405124): -.
10. [10]
  Yongpo Zhang , Xinfeng Li , Yafei Song , Mengyao Sun , Congcong Yin , Chunyan Gao , Jinzhong Zhao . Synthesis of Chlorine-Bridged Binuclear Cu(I) Complexes Based on Conjugation-Driven Cu(II) Oxidized Secondary Amines. University Chemistry, 2024, 39(5): 44-51. doi: 10.3866/PKU.DXHX202309092
11. [11]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
12. [12]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
13. [13]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
14. [14]
  Zhaomei LIU , Wenshi ZHONG , Jiaxin LI , Gengshen HU . Preparation of nitrogen-doped porous carbons with ultra-high surface areas for high-performance supercapacitors. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 677-685. doi: 10.11862/CJIC.20230404
15. [15]
  Zhen Yao , Bing Lin , Youping Tian , Tao Li , Wenhui Zhang , Xiongwei Liu , Wude Yang . Visible-Light-Mediated One-Pot Synthesis of Secondary Amines and Mechanistic Exploration. University Chemistry, 2024, 39(5): 201-208. doi: 10.3866/PKU.DXHX202311033
16. [16]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
17. [17]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
18. [18]
  Jiandong Liu , Zhijia Zhang , Mikhail Kamenskii , Filipp Volkov , Svetlana Eliseeva , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-. doi: 10.3866/PKU.WHXB202308048
19. [19]
  Yinuo Wang , Siran Wang , Yilong Zhao , Dazhen Xu . Selective Synthesis of Diarylmethyl Anilines and Triarylmethanes via Multicomponent Reactions: Introduce a Comprehensive Experiment of Organic Chemistry. University Chemistry, 2024, 39(8): 324-330. doi: 10.3866/PKU.DXHX202401063
20. [20]
  Yi Yang , Xin Zhou , Miaoli Gu , Bei Cheng , Zhen Wu , Jianjun Zhang . S型ZnO/CdIn₂S₄光催化剂制备H₂O₂偶联苄胺氧化的超快电子转移飞秒吸收光谱研究. Acta Physico-Chimica Sinica, 2025, 41(6): 100064-. doi: 10.1016/j.actphy.2025.100064

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(640)
HTML views(67)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142