Citation: ZHANG Peng, SUN Changlong, ZHANG Ren, GAO Mingxia, ZHANG Xiangmin. A novel and facile microchip based on nitrocellulose membrane toward efficient capture of circulating tumor cells[J]. Chinese Journal of Chromatography, ;2013, 31(6): 518-521. doi: 10.3724/SP.J.1123.2013.01022 shu

A novel and facile microchip based on nitrocellulose membrane toward efficient capture of circulating tumor cells

ZHANG Peng ¹ ,
SUN Changlong ¹ ,
ZHANG Ren ¹ ,
GAO Mingxia ^1,2,, ,
ZHANG Xiangmin ^1,2,,

1.
1. Department of Chemistry, Fudan University, Shanghai 200433, China;

Corresponding author: GAO Mingxia, ZHANG Xiangmin,
Received Date: 10 January 2013
Available Online: 25 February 2013
Fund Project: 科技部基础研究计划项目(2012CB910604) (2012CB910604)国家自然科学基金项目(21275034,21027002,21175026). (21275034,21027002,21175026)

A novel and facile circulating tumor cell (CTC) microchip has been developed for the isolation and detection of cancer cells. The CTC microchip was prepared based on the nitrocellulose membrane substrate, which shows high affinity to proteins and hence can adsorb antibodies naturally. We employed non-small-cells of lung cancer NCI-H1650 as target cells and testified the high capture efficacy of the CTC microchip. Furthermore, we spiked 500 cancer cells to 1 mL healthy donor’s whole blood in order to simulate the detection of CTC in patient and detected 182 cancer cells ultimately, indicating the huge application potential in the future.
- nitrocellulose membrane (NCM),
- microchip,
- circulating tumor cells (CTCs),
- antibody
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