Citation: CHI Yumei, LI Yao, ZHANG Yu, WANG Qinxia, CUI Xiaobing. Analysis of flavonoids and components of stems & leaves of Ranunculus Ternatus Thunb. using ultra performance liquid chromatography-quadrupole tandem time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(9): 838-844. doi: 10.3724/SP.J.1123.2013.01009 shu

Analysis of flavonoids and components of stems & leaves of Ranunculus Ternatus Thunb. using ultra performance liquid chromatography-quadrupole tandem time of flight mass spectrometry

CHI Yumei ^1,, ,
LI Yao ² ,
ZHANG Yu ^1,2 ,
WANG Qinxia ¹ ,
CUI Xiaobing ¹

1.
1. Nanjing University of Chinese Medicine, Nanjing 210023, China;

Corresponding author: CHI Yumei,
Received Date: 5 January 2013
Available Online: 23 April 2013
Fund Project: 国家自然科学基金项目(20475027). (20475027)

In order to explore the applications of liquid chromatography-mass spectrometry (LC-MS) technology in the rapid identification of components of Chinese herbal medicines and natural products, reference flavones were used as precursors, and the stems and leaves of medicinal plant Ranunculus ternatus Thunb. were used as the objects. Ultra performance liquid chromatography with a diode array detector-electrospray ionization quadrupole tandem time of flight mass spectrometry (UPLC/DAD-ESI/Q-TOF MS) was also used to analyse the characteristics of flavonoid homologues and flavonoid isomers. The results showed that ultraviolet (UV) absorption and MS/MS spectra of C-glycosyl were distinct from O-glycosyl. There was also a correlation between glycosidation positions and the retention times, MS/MS fragments and their relative abundances. When applied it to analyse the alcohol extract of stems and leaves of Ranunculus ternatus Thunb. and their acid hydrolysis solution, 22 flavonol glycosides and 3 flavonoid aglycones were identified. The method is simple, convenient and exercisable.
1. [1]
  [1] Zeng J, Zhang X L, Guo Z M, et al. J Chromatogr A, 2012, 1220: 50
2. [2]
  [2] Vukics V, Ringer T, Kery A, et al. J Chromatogr A, 2008, 1206(1): 11
3. [3]
  [3] Colombo R, Yariwake J H, McCullagh M. J Braz Chem Soc, 2008, 19(3): 483
4. [4]
  [4] Ferreres F, Gil-Izquierdo A, Andrade P B, et al. J Chromatogr A, 2007, 1161: 214
5. [5]
  [5] The Pharmacopoeia Committee of the People's Republic of China. Pharmacopoeia of People's Republic of China: Part 1. Beijing: China Medical Science Press (国家药典委员会. 中华人民共和国药典: 一部. 北京: 中国医药科技出版社), 2010
6. [6]
  [6] Zhang Y, Chi Y M, Tan X H, et al. The National Symposium on Natural Medicinal Material Resources Proceedings and Abstracts (张瑜, 池玉梅, 谈献和, 等. 全国第9届天然药物资源学术研讨会论文集), 2010: 534
7. [7]
  [7] Chi Y M, Yu S, Guo R, et al. Journal of Jiangsu Traditional Chinese Medicine (池玉梅, 于生, 郭戎, 等. 江苏中医药),2007, 39(12): 56
8. [8]
  [8] Xu R S. Natural Products Chemistry. Beijing: Science Press (徐任生. 天然产物化学. 北京: 科学出版社), 1997
9. [9]
  [9] Xiao C H. Chinese Medicine Chemistry. Shanghai: Shanghai Science and Technology Press (肖崇厚. 中药化学. 上海: 上海科学技术出版社), 1998
10. [10]
  [10] Chi Y M, Zhu H Y, Ju L, et al. Chinese Journal of Analytical Chemistry (池玉梅, 朱华云, 居羚, 等. 分析化学), 2009, 37(2): 227
11. [11]
  [11] Chi Y M, Pan S H. Chinese Journal of Food Hygiene (池玉梅, 潘苏华. 中国食品卫生杂志), 2001, 13(1): 21
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Geyang Song , Dong Xue , Gang Li . Recent Advances in Transition Metal-Catalyzed Synthesis of Anilines from Aryl Halides. University Chemistry, 2024, 39(2): 321-329. doi: 10.3866/PKU.DXHX202308030
3. [3]
  Aidang Lu , Yunting Liu , Yanjun Jiang . Comprehensive Organic Chemistry Experiment: Synthesis and Characterization of Triazolopyrimidine Compounds. University Chemistry, 2024, 39(8): 241-246. doi: 10.3866/PKU.DXHX202401029
4. [4]
  Qi Zhang , Ziyu Liu , Hongxia Tan , Jun Tong , Dazhen Xu . Research Progress on Direct Synthesis of β-Hydroxy Sulfones via Difunctionalization of Olefins. University Chemistry, 2025, 40(11): 199-209. doi: 10.12461/PKU.DXHX202412064
5. [5]
  Feng Han , Fuxian Wan , Ying Li , Congcong Zhang , Yuanhong Zhang , Chengxia Miao . Comprehensive Organic Chemistry Experiment: Phosphotungstic Acid-Catalyzed Direct Conversion of Triphenylmethanol for the Synthesis of Oxime Ethers. University Chemistry, 2025, 40(3): 342-348. doi: 10.12461/PKU.DXHX202405181
6. [6]
  Lili Jiang , Shaoyu Zheng , Xuejiao Liu , Xiaomin Xie . Copper-Catalyzed Oxidative Coupling Reactions for the Synthesis of Aryl Sulfones: A Fundamental and Exploratory Experiment for Undergraduate Teaching. University Chemistry, 2025, 40(7): 267-276. doi: 10.12461/PKU.DXHX202408004
7. [7]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
8. [8]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
9. [9]
  Qianlang Wang , Jijun Sun , Qian Chen , Quanqin Zhao , Baojuan Xi . The Appeal of Organophosphorus Compounds: Clearing Their Name. University Chemistry, 2025, 40(4): 299-306. doi: 10.12461/PKU.DXHX202405205
10. [10]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
11. [11]
  Chi Li , Jichao Wan , Qiyu Long , Hui Lv , Ying Xiong . N-Heterocyclic Carbene (NHC)-Catalyzed Amidation of Aldehydes with Nitroso Compounds. University Chemistry, 2024, 39(5): 388-395. doi: 10.3866/PKU.DXHX202312016
12. [12]
  Ying Xiong , Guangao Yu , Lin Wu , Qingwen Liu , Houjin Li , Shuanglian Cai , Zhanxiang Liu , Xingwen Sun , Yuan Zheng , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Determination of Physical Constants of Organic Compounds. University Chemistry, 2025, 40(5): 106-121. doi: 10.12461/PKU.DXHX202503079
13. [13]
  Yongjian Zhang , Fangling Gao , Hong Yan , Keyin Ye . Electrochemical Transformation of Organosulfur Compounds. University Chemistry, 2025, 40(5): 311-317. doi: 10.12461/PKU.DXHX202407035
14. [14]
  Nan Xiao , Fang Sun . 二芳基硫醚化合物的构建及应用. University Chemistry, 2025, 40(6): 360-363. doi: 10.12461/PKU.DXHX202407099
15. [15]
  Yerong Chen , Bingbin Yang , Xinglei He , Yuqi Lin , Keyin Ye . Enzyme-Directed Evolution Enables Bioconversion of Organosilicon Compounds. University Chemistry, 2025, 40(10): 121-129. doi: 10.12461/PKU.DXHX202411054
16. [16]
  Qiwen Chen , Baolei Wang . Research Progress on One-Electron σ-Bond of Organic Compounds. University Chemistry, 2025, 40(11): 191-198. doi: 10.12461/PKU.DXHX202412136
17. [17]
  Jiaming Xu , Yu Xiang , Weisheng Lin , Zhiwei Miao . Research Progress in the Synthesis of Cyclic Organic Compounds Using Bimetallic Relay Catalytic Strategies. University Chemistry, 2024, 39(3): 239-257. doi: 10.3866/PKU.DXHX202309093
18. [18]
  Xilin Zhao , Xingyu Tu , Zongxuan Li , Rui Dong , Bo Jiang , Zhiwei Miao . Research Progress in Enantioselective Synthesis of Axial Chiral Compounds. University Chemistry, 2024, 39(11): 158-173. doi: 10.12461/PKU.DXHX202403106
19. [19]
  Hanxue LIU , Shijie LI , Meng REN , Xuling XUE , Hongke LIU . Design and antitumor properties of dehydroabietic acid functionalized cyclometalated iridium(Ⅲ) complex. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1483-1494. doi: 10.11862/CJIC.20250031
20. [20]
  Xiaodong Chen , Yumin Zhang . An Improved Simulated Annealing Algorithm for Predicting the Molecular Formulas of Organic Compounds. University Chemistry, 2025, 40(9): 19-24. doi: 10.12461/PKU.DXHX202408095

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(640)
HTML views(102)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142